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The map label for this gene is potD [C]

Identifier: 51894354

GI number: 51894354

Start: 3433620

End: 3434696

Strand: Reverse

Name: potD [C]

Synonym: STH3219

Alternate gene names: 51894354

Gene position: 3434696-3433620 (Counterclockwise)

Preceding gene: 51894355

Following gene: 51894353

Centisome position: 96.31

GC content: 65.18

Gene sequence:

>1077_bases
ATGTGGAAGCGGACTCTGGCTCTCACCCTGGCCGCGAGCCTCCTGGTTCTCGCCGGCTGCGGCTCGAAGCAGACTCCGGC
CCCGTCGACCACGCCTTCGACTTCGAACCCGCCCGCGTCATCCGAGGCCCCGAAGTCGAACGAACCCAAGAAGCTGGTGA
TCTACGCCGGCATGATGGAGGACCATGTGAAGGGTGCCGTGGCCGAGTTTGAGGCCCAGACCGGCATCAAGGTGGAATGG
GTGCGCATGTCCTCCGGCGAGACGCTGACCCGGATCCGGGCCGAGAAGGAGAACCCGCAGGCCAGCGTCTGGTACGGCGG
TCCGGCGGACGCCTACGTGGCGGCCAAGGAGGAGGGGCTCCTGGCGCAGTACGTCTCGCCCAACGCCGCTAAGATTCCCG
ATCAGTTCAAGGATCCCGAGGGCTACTGGACCGGCATTTACGTCGGCCTCCTGGGCTTCGGCTACAACACGAAGATGATG
GAAGAGAAGGGGCTCACCCCGCCTGAGTCGTGGGAGGACCTGCTGAAGCCCGAGTTCAAGGGCCAGATCGCCCTGGCCAA
CCCCGGTTCGTCGGGCACCTCCTACACCCTGCTGGCCACCATCGTCCAGCTGATGGGTGAGGAGAAGGGCCTGGAGTACA
TGACCAAGCTGCACGAGCAGGTCGCCCAGTATCCCAAGTCGGGTACCGCCCCGGGCCAGATGGCCGGCCGCGGCGAGGTG
CTCATCGGGATCTCGTTCGCTCACGACATCGTGAAGTACGCCAAGGAGGGCATGCCCCTCGACGTCACCTTCCCGAAGGA
GGGCACCGGCTACGAGATTGGCGGCATCGCCCTGATCAACGGCGCTCCCGACGAGGAGGCGGCCAAGATCTTCATCGACT
GGGCCCTGACCAAGGAGGCGCAGGAGCTCGGCCAGAAGTACGGCTCCTACCAGTCGCTGACCAACGTGGAGGCGACCAAT
CCGCCCGAGGCGTTCTCGCTGGACGAGGTCACGATCATCGACTACGACCTGCAGTGGGCGGGCGCCAACCGGACCCAGCT
CGTCGAGAAGTGGTCCAAGGCCGTCAATATGCAGTAG

Upstream 100 bases:

>100_bases
GTACGCGAGGCTACGGATACACGGAAAGGTCGTTCGAATGTACGATTACACTGTCTCCTACTTTTGGAGGATCTCTCTCA
CGCATACGGAGGGAAGCATG

Downstream 100 bases:

>100_bases
CACAGTACGGAAGGGCCGGGCTCTCCAGCCCGGCCCTTGACGTATCGAGGAGGGTGTAACACGTGGCCTCTATCGCCGCC
CGCCGCAAGCGCAGCGCCTC

Product: iron ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MWKRTLALTLAASLLVLAGCGSKQTPAPSTTPSTSNPPASSEAPKSNEPKKLVIYAGMMEDHVKGAVAEFEAQTGIKVEW
VRMSSGETLTRIRAEKENPQASVWYGGPADAYVAAKEEGLLAQYVSPNAAKIPDQFKDPEGYWTGIYVGLLGFGYNTKMM
EEKGLTPPESWEDLLKPEFKGQIALANPGSSGTSYTLLATIVQLMGEEKGLEYMTKLHEQVAQYPKSGTAPGQMAGRGEV
LIGISFAHDIVKYAKEGMPLDVTFPKEGTGYEIGGIALINGAPDEEAAKIFIDWALTKEAQELGQKYGSYQSLTNVEATN
PPEAFSLDEVTIIDYDLQWAGANRTQLVEKWSKAVNMQ

Sequences:

>Translated_358_residues
MWKRTLALTLAASLLVLAGCGSKQTPAPSTTPSTSNPPASSEAPKSNEPKKLVIYAGMMEDHVKGAVAEFEAQTGIKVEW
VRMSSGETLTRIRAEKENPQASVWYGGPADAYVAAKEEGLLAQYVSPNAAKIPDQFKDPEGYWTGIYVGLLGFGYNTKMM
EEKGLTPPESWEDLLKPEFKGQIALANPGSSGTSYTLLATIVQLMGEEKGLEYMTKLHEQVAQYPKSGTAPGQMAGRGEV
LIGISFAHDIVKYAKEGMPLDVTFPKEGTGYEIGGIALINGAPDEEAAKIFIDWALTKEAQELGQKYGSYQSLTNVEATN
PPEAFSLDEVTIIDYDLQWAGANRTQLVEKWSKAVNMQ
>Mature_358_residues
MWKRTLALTLAASLLVLAGCGSKQTPAPSTTPSTSNPPASSEAPKSNEPKKLVIYAGMMEDHVKGAVAEFEAQTGIKVEW
VRMSSGETLTRIRAEKENPQASVWYGGPADAYVAAKEEGLLAQYVSPNAAKIPDQFKDPEGYWTGIYVGLLGFGYNTKMM
EEKGLTPPESWEDLLKPEFKGQIALANPGSSGTSYTLLATIVQLMGEEKGLEYMTKLHEQVAQYPKSGTAPGQMAGRGEV
LIGISFAHDIVKYAKEGMPLDVTFPKEGTGYEIGGIALINGAPDEEAAKIFIDWALTKEAQELGQKYGSYQSLTNVEATN
PPEAFSLDEVTIIDYDLQWAGANRTQLVEKWSKAVNMQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38797; Mature: 38797

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKRTLALTLAASLLVLAGCGSKQTPAPSTTPSTSNPPASSEAPKSNEPKKLVIYAGMME
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHH
DHVKGAVAEFEAQTGIKVEWVRMSSGETLTRIRAEKENPQASVWYGGPADAYVAAKEEGL
HHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHEHHCCCCCEEEEECCCCCCEEEECCCCC
LAQYVSPNAAKIPDQFKDPEGYWTGIYVGLLGFGYNTKMMEEKGLTPPESWEDLLKPEFK
EEECCCCCCCCCCHHCCCCCCCCHHHEEEHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCC
GQIALANPGSSGTSYTLLATIVQLMGEEKGLEYMTKLHEQVAQYPKSGTAPGQMAGRGEV
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LIGISFAHDIVKYAKEGMPLDVTFPKEGTGYEIGGIALINGAPDEEAAKIFIDWALTKEA
EEEEHHHHHHHHHHHCCCCEEEECCCCCCCEEECCEEEECCCCCCCCEEEEEEEHHHHHH
QELGQKYGSYQSLTNVEATNPPEAFSLDEVTIIDYDLQWAGANRTQLVEKWSKAVNMQ
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWKRTLALTLAASLLVLAGCGSKQTPAPSTTPSTSNPPASSEAPKSNEPKKLVIYAGMME
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHH
DHVKGAVAEFEAQTGIKVEWVRMSSGETLTRIRAEKENPQASVWYGGPADAYVAAKEEGL
HHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHEHHCCCCCEEEEECCCCCCEEEECCCCC
LAQYVSPNAAKIPDQFKDPEGYWTGIYVGLLGFGYNTKMMEEKGLTPPESWEDLLKPEFK
EEECCCCCCCCCCHHCCCCCCCCHHHEEEHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCC
GQIALANPGSSGTSYTLLATIVQLMGEEKGLEYMTKLHEQVAQYPKSGTAPGQMAGRGEV
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LIGISFAHDIVKYAKEGMPLDVTFPKEGTGYEIGGIALINGAPDEEAAKIFIDWALTKEA
EEEEHHHHHHHHHHHCCCCEEEECCCCCCCEEECCEEEECCCCCCCCEEEEEEEHHHHHH
QELGQKYGSYQSLTNVEATNPPEAFSLDEVTIIDYDLQWAGANRTQLVEKWSKAVNMQ
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]