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The map label for this gene is mjaVM [H]

Identifier: 51893663

GI number: 51893663

Start: 2710926

End: 2711840

Strand: Reverse

Name: mjaVM [H]

Synonym: STH2525

Alternate gene names: 51893663

Gene position: 2711840-2710926 (Counterclockwise)

Preceding gene: 51893664

Following gene: 51893662

Centisome position: 76.04

GC content: 65.46

Gene sequence:

>915_bases
TTGCAATGCGCGCCAATGATTAGGAGGAGTTTCGCGCCGCGGCGCGAAATAATACCAGGCAGAACCATTGCAGACGTTCG
TATTCGATCGCCCCGACCCGCCGGTCGGAGGATGACGGGAGGCCGGTGCATGTTCGCCAATCGGATCTACAACGCTGATG
CCCGCAACATGAGCTTCATCCCCGACGGGGTGGTGGACCTGGTCGTCACCTCACCGCCTTATAACGTCGGCAAGAACTAC
GCCACCCACGACGACTGCCTCTCGATGGAGGAGTACCTGGACCTGCTGGAGCAGGTGTGGCGGGAGTGCTACCGCGTCCT
GGCGCCGGGCGGCCGCATCGCGATCAACGTGGCCGGGGTCGACCGGAAGCCCTACCTGCCCCTGCACGCGTACATCACGC
TTCAGATGATCCGGCTGGGCTTCCAGATGCGGGGCGAGATCATCTGGAACAAGGGCGCGTCCGTGGGCGTCTCCACCGCC
TGGGGGTCCTGGTGCAGTCCGTCCAACCCGACCCTGCGTGACCTGCACGAGTACATCCTGGTCTTCTCCAAGGAAGACTG
GCGCATGGGGCACCGGGGCGAGACCGACCTGACGCCCGAGGAGTTCGTGACGTACACGAAATCGATCTGGGAGTTTCCGA
CGGTATCGGCCAAGAAGGTCGGCCACCCCGCCCCCTTCCCGCTGGAGTTGCCCAGCCGGCTGATCAAACTCTACACTTAT
AAAGGCGACCTGGTGCTGGATCCCTTCAACGGCAGCGGCACCACCTGCCAGGCCGCGGCGTTGCTCGGGCGCCGCTGGAT
CGGCGTTGACATCGACCCCGGCTACTGCGCCCTGGCGGAGAAGAACATGCGCACCCTGGTGGACGGCGCAGGCGGAGCCG
GGCTCGGCGCCGCCTCGGGCGTGGCCGGGGCCTGA

Upstream 100 bases:

>100_bases
GATCTTGTTGTAAGGGCTGCACGGGGCATCCGACCGGGGGAGCGATCCCCCGGTCTTTTGCTGCCCTGCGGGCTTCGGGC
GACGGGCGTCTGCATATCCT

Downstream 100 bases:

>100_bases
CGTCGGGAGGGGTGGGGATGATGGCAGGCCAACCGATCCCGTTTCTGCGGCCGGCCTTGCATGCGCTGGTGCGCGGGCCG
TGGCGGACGCTCTGGATCGT

Product: type II restriction-modification system DNA methylase

Products: NA

Alternate protein names: M.MjaV; N-4 cytosine-specific methyltransferase MjaV [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQCAPMIRRSFAPRREIIPGRTIADVRIRSPRPAGRRMTGGRCMFANRIYNADARNMSFIPDGVVDLVVTSPPYNVGKNY
ATHDDCLSMEEYLDLLEQVWRECYRVLAPGGRIAINVAGVDRKPYLPLHAYITLQMIRLGFQMRGEIIWNKGASVGVSTA
WGSWCSPSNPTLRDLHEYILVFSKEDWRMGHRGETDLTPEEFVTYTKSIWEFPTVSAKKVGHPAPFPLELPSRLIKLYTY
KGDLVLDPFNGSGTTCQAAALLGRRWIGVDIDPGYCALAEKNMRTLVDGAGGAGLGAASGVAGA

Sequences:

>Translated_304_residues
MQCAPMIRRSFAPRREIIPGRTIADVRIRSPRPAGRRMTGGRCMFANRIYNADARNMSFIPDGVVDLVVTSPPYNVGKNY
ATHDDCLSMEEYLDLLEQVWRECYRVLAPGGRIAINVAGVDRKPYLPLHAYITLQMIRLGFQMRGEIIWNKGASVGVSTA
WGSWCSPSNPTLRDLHEYILVFSKEDWRMGHRGETDLTPEEFVTYTKSIWEFPTVSAKKVGHPAPFPLELPSRLIKLYTY
KGDLVLDPFNGSGTTCQAAALLGRRWIGVDIDPGYCALAEKNMRTLVDGAGGAGLGAASGVAGA
>Mature_304_residues
MQCAPMIRRSFAPRREIIPGRTIADVRIRSPRPAGRRMTGGRCMFANRIYNADARNMSFIPDGVVDLVVTSPPYNVGKNY
ATHDDCLSMEEYLDLLEQVWRECYRVLAPGGRIAINVAGVDRKPYLPLHAYITLQMIRLGFQMRGEIIWNKGASVGVSTA
WGSWCSPSNPTLRDLHEYILVFSKEDWRMGHRGETDLTPEEFVTYTKSIWEFPTVSAKKVGHPAPFPLELPSRLIKLYTY
KGDLVLDPFNGSGTTCQAAALLGRRWIGVDIDPGYCALAEKNMRTLVDGAGGAGLGAASGVAGA

Specific function: This methylase recognizes the double-stranded sequence GTAC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaV endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=249, Percent_Identity=30.1204819277108, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 33627; Mature: 33627

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCAPMIRRSFAPRREIIPGRTIADVRIRSPRPAGRRMTGGRCMFANRIYNADARNMSFI
CCCHHHHHHHCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCC
PDGVVDLVVTSPPYNVGKNYATHDDCLSMEEYLDLLEQVWRECYRVLAPGGRIAINVAGV
CCCEEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DRKPYLPLHAYITLQMIRLGFQMRGEIIWNKGASVGVSTAWGSWCSPSNPTLRDLHEYIL
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHE
VFSKEDWRMGHRGETDLTPEEFVTYTKSIWEFPTVSAKKVGHPAPFPLELPSRLIKLYTY
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHEEE
KGDLVLDPFNGSGTTCQAAALLGRRWIGVDIDPGYCALAEKNMRTLVDGAGGAGLGAASG
CCCEEEECCCCCCCHHHHHHHHCCCEEEEECCCCEEEEHHCCHHHHHCCCCCCCCCCCCC
VAGA
CCCC
>Mature Secondary Structure
MQCAPMIRRSFAPRREIIPGRTIADVRIRSPRPAGRRMTGGRCMFANRIYNADARNMSFI
CCCHHHHHHHCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCC
PDGVVDLVVTSPPYNVGKNYATHDDCLSMEEYLDLLEQVWRECYRVLAPGGRIAINVAGV
CCCEEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DRKPYLPLHAYITLQMIRLGFQMRGEIIWNKGASVGVSTAWGSWCSPSNPTLRDLHEYIL
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHE
VFSKEDWRMGHRGETDLTPEEFVTYTKSIWEFPTVSAKKVGHPAPFPLELPSRLIKLYTY
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHEEE
KGDLVLDPFNGSGTTCQAAALLGRRWIGVDIDPGYCALAEKNMRTLVDGAGGAGLGAASG
CCCEEEECCCCCCCHHHHHHHHCCCEEEEECCCCEEEEHHCCHHHHHCCCCCCCCCCCCC
VAGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]