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The map label for this gene is sbcD [H]

Identifier: 51893314

GI number: 51893314

Start: 2329241

End: 2330476

Strand: Reverse

Name: sbcD [H]

Synonym: STH2176

Alternate gene names: 51893314

Gene position: 2330476-2329241 (Counterclockwise)

Preceding gene: 51893315

Following gene: 51893313

Centisome position: 65.35

GC content: 73.06

Gene sequence:

>1236_bases
GTGCGCATCCTCCATACCGCCGACTGGCACCTCGGCCGCACCTTGGAAGGCCGCAGCCGGCAGGAAGAGCACGAAGCGTT
CGTGGACGAGCTCTGCGCCATGGTGCGCGAGGAGCGGATCGACCTGGTGCTCATCGCCGGCGACGTGTTTGACACGGGCA
ACCCCTCGGCGGCGGCCGAGGAGCTTTACTGCGACGCCCTGGCCCGGTTGGGCGAGAACGGCCGGCGGGCCGTGGTGGTC
ATCGCCGGCAACCACGACTCCCCCGACCGGCTGACCGCCGTCCAGGCGCTGGCCCAGCGGCACGGGGCCACGATGTTCGG
CTATCCCTACGACGACCCGGGCGTCTACACCCCCGGGCCGGACCGGGTGCAGCGGGTGGCGGCCGGCCCCGGCTGGGCCG
AGCTGACCGTCCCCGGCGTGGACCACTCCGCCGTGGTCCTGGCCCTCCCGTACCCTTCGGAGTCCCGGCTCCGGAAGCTC
ATCGCCGACACCCTGGGGGAGGAGGAGCTGCAGCGCTCCTACTCCGACCAGGTGAAGGGCTGGCTGGCGGCGGCGGCAGG
CCGTTTCCGGCCGGATGCGGTGCGGCTGGTCACGAGCCACCTGTACATGGCCGGCGGCGTCGAGTCCGACGTGGTCGAGC
GGCCGATCCAGATGGGCGGCGCGTACACGGTGCACCCCTGCGCCGTGCCGGAGACCGCCCAGTACGTGGCCCTCGGCCAC
CTGCACCGTCCCCAGGAGATCCACGGGACGGCGGCGGTCACCCGCTACTCGGGCTCCCCGATCTCCTTCTCCTTCAACGA
GACCGGTTACGTCAAGGGCGTGACCGTCGTCGAGGTGGAGCCCCGGGATCCCGCGCCCCGGGTGCGGCACGTGCCCATCT
CCGCCGGGCGGCCCCTGGTGCGCTGGGTCGCCGAGGACGGCCTGGCCCAGGTGGAGCGCTGGGTGGCGGAGGGGAAGGAT
CCGACGGCCTGGATCTCGCTGGAGGTCCACACGGAGCATCCGCTTACCCTGGAGCAGGTGCACCGGCTGCGGGCGATGCG
GCCGGAGTTCGTGCACATCCGGGCCGTCCTCCCGGCCGAGGCCGAGGCCGCCGCGACCGCGGAGCCGCAGCGGCTGGGGG
TGGACGAGCAGTTCGTCCGGTTCTACTCCCAGGTGCGGGGCGGCCCGCCCGCCCCTGAGCTGGTCCGGCTCTTCCTGGAG
CTGGTCAACGCCCGGGACGAGGAGGTGGACGCATGA

Upstream 100 bases:

>100_bases
GCCGGTTTCCATCCTCAAGTCGCCCGCGAGGAGGAAGGAGAACACCCGTTCCGCCTCGAACCTCTCTGCCAAGCTTACCA
GAAGATGAGGAGGAGAACGC

Downstream 100 bases:

>100_bases
GGCCCATCCGGCTGGCGTTTGCGGGGCTGCAGAGCTACCGGGAGCCCCAGGAGATCGACTTCCGCGAGCTGATGGGCGCC
GGCCTCTTCGGCATCTTCGG

Product: DNA repair exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MRILHTADWHLGRTLEGRSRQEEHEAFVDELCAMVREERIDLVLIAGDVFDTGNPSAAAEELYCDALARLGENGRRAVVV
IAGNHDSPDRLTAVQALAQRHGATMFGYPYDDPGVYTPGPDRVQRVAAGPGWAELTVPGVDHSAVVLALPYPSESRLRKL
IADTLGEEELQRSYSDQVKGWLAAAAGRFRPDAVRLVTSHLYMAGGVESDVVERPIQMGGAYTVHPCAVPETAQYVALGH
LHRPQEIHGTAAVTRYSGSPISFSFNETGYVKGVTVVEVEPRDPAPRVRHVPISAGRPLVRWVAEDGLAQVERWVAEGKD
PTAWISLEVHTEHPLTLEQVHRLRAMRPEFVHIRAVLPAEAEAAATAEPQRLGVDEQFVRFYSQVRGGPPAPELVRLFLE
LVNARDEEVDA

Sequences:

>Translated_411_residues
MRILHTADWHLGRTLEGRSRQEEHEAFVDELCAMVREERIDLVLIAGDVFDTGNPSAAAEELYCDALARLGENGRRAVVV
IAGNHDSPDRLTAVQALAQRHGATMFGYPYDDPGVYTPGPDRVQRVAAGPGWAELTVPGVDHSAVVLALPYPSESRLRKL
IADTLGEEELQRSYSDQVKGWLAAAAGRFRPDAVRLVTSHLYMAGGVESDVVERPIQMGGAYTVHPCAVPETAQYVALGH
LHRPQEIHGTAAVTRYSGSPISFSFNETGYVKGVTVVEVEPRDPAPRVRHVPISAGRPLVRWVAEDGLAQVERWVAEGKD
PTAWISLEVHTEHPLTLEQVHRLRAMRPEFVHIRAVLPAEAEAAATAEPQRLGVDEQFVRFYSQVRGGPPAPELVRLFLE
LVNARDEEVDA
>Mature_411_residues
MRILHTADWHLGRTLEGRSRQEEHEAFVDELCAMVREERIDLVLIAGDVFDTGNPSAAAEELYCDALARLGENGRRAVVV
IAGNHDSPDRLTAVQALAQRHGATMFGYPYDDPGVYTPGPDRVQRVAAGPGWAELTVPGVDHSAVVLALPYPSESRLRKL
IADTLGEEELQRSYSDQVKGWLAAAAGRFRPDAVRLVTSHLYMAGGVESDVVERPIQMGGAYTVHPCAVPETAQYVALGH
LHRPQEIHGTAAVTRYSGSPISFSFNETGYVKGVTVVEVEPRDPAPRVRHVPISAGRPLVRWVAEDGLAQVERWVAEGKD
PTAWISLEVHTEHPLTLEQVHRLRAMRPEFVHIRAVLPAEAEAAATAEPQRLGVDEQFVRFYSQVRGGPPAPELVRLFLE
LVNARDEEVDA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=350, Percent_Identity=31.4285714285714, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45184; Mature: 45184

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTADWHLGRTLEGRSRQEEHEAFVDELCAMVREERIDLVLIAGDVFDTGNPSAAAE
CCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
ELYCDALARLGENGRRAVVVIAGNHDSPDRLTAVQALAQRHGATMFGYPYDDPGVYTPGP
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCH
DRVQRVAAGPGWAELTVPGVDHSAVVLALPYPSESRLRKLIADTLGEEELQRSYSDQVKG
HHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
WLAAAAGRFRPDAVRLVTSHLYMAGGVESDVVERPIQMGGAYTVHPCAVPETAQYVALGH
HHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHC
LHRPQEIHGTAAVTRYSGSPISFSFNETGYVKGVTVVEVEPRDPAPRVRHVPISAGRPLV
CCCCHHHCCCEEEEEECCCCEEEECCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCHHH
RWVAEDGLAQVERWVAEGKDPTAWISLEVHTEHPLTLEQVHRLRAMRPEFVHIRAVLPAE
HHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCC
AEAAATAEPQRLGVDEQFVRFYSQVRGGPPAPELVRLFLELVNARDEEVDA
CCHHCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRILHTADWHLGRTLEGRSRQEEHEAFVDELCAMVREERIDLVLIAGDVFDTGNPSAAAE
CCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
ELYCDALARLGENGRRAVVVIAGNHDSPDRLTAVQALAQRHGATMFGYPYDDPGVYTPGP
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCH
DRVQRVAAGPGWAELTVPGVDHSAVVLALPYPSESRLRKLIADTLGEEELQRSYSDQVKG
HHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
WLAAAAGRFRPDAVRLVTSHLYMAGGVESDVVERPIQMGGAYTVHPCAVPETAQYVALGH
HHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHC
LHRPQEIHGTAAVTRYSGSPISFSFNETGYVKGVTVVEVEPRDPAPRVRHVPISAGRPLV
CCCCHHHCCCEEEEEECCCCEEEECCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCHHH
RWVAEDGLAQVERWVAEGKDPTAWISLEVHTEHPLTLEQVHRLRAMRPEFVHIRAVLPAE
HHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCC
AEAAATAEPQRLGVDEQFVRFYSQVRGGPPAPELVRLFLELVNARDEEVDA
CCHHCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]