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The map label for this gene is kinE [H]

Identifier: 51893226

GI number: 51893226

Start: 2227339

End: 2229504

Strand: Reverse

Name: kinE [H]

Synonym: STH2088

Alternate gene names: 51893226

Gene position: 2229504-2227339 (Counterclockwise)

Preceding gene: 51893227

Following gene: 51893225

Centisome position: 62.52

GC content: 72.35

Gene sequence:

>2166_bases
ATGACCCGCTGGTTCGGCCACCTGCGGTTGAAGGTGCTCGCGTTCGGCATCGCCATGTCGGTCCTTCCCGTCTCGATCTA
CGGCTGGTACGGACTGGCTGCGGCCCGCACCGCGCAGGTGGAGGTGGTGCAGGCGCAGAACCAGGCCGCTGCGCGCACGG
TCTCCGAGGAGCTGGCCCGGTTCGTCGAGCGCATCACCGGGCAGCTGCAGCTGCTCGCCCGCCTGGAGGGCGCCGGGCTG
GTTTCGGCCCCCGCGGCCGAGCAGGAGCGGGTGCTCTACACCCTGCTCCGGGACACGCCTTACCTGGAGGAGGTGTCGCT
GGTCGACGCCTCAGGCGCGGAGGTGTCCCGGGCATCCCGGCGGGAGGTCGTGGCCGGTCAGCCGGCCGCTCGATACAGCG
GTGAGCCCTACTGGGCCGATCTCCGGCGCGGCGAATCCGTCGTGGGGCCGGTCACCTTCGACCCGCACGGCCGCCCGCTC
TTCACCGTGGGCGTGCCGCTCCCCGACGGCGCCGGCGCCCTGATCGCCCGGCCCACGCTGCGGGGGCTGGTGGACAACCT
GGCGGCCGTCAGGGCCGGTGGAGCCGTGCGGACGCACGTCATCGACGAAACCGGGCGCCTGATCGGCGACTCGGACTTCA
GCCTCGTGCTGGCCGGTTACCGGTGGGCGGGTGTGCCCGCACCTGACCAGCCGTACGGCTCCCTCACCGGCGAGGAGGCG
TTCGGCTACCTCGCCGAGGTGCCTGGACTGGGGTGGCGGGTGCTTGCGGAGACCACGGTGGATGACGCCATGGTCCCTGT
CCGAAGGCTGGCCTGGGAGTTCGCAGGCGGGGCCCTGCTGATGGTCTCCGTCGTGGTCGGCCTCTCCATCGTGTTCGGCC
TGCAGCTCACGGTGCCTTTGGAGCGGCTGGAGGCGGGGGCCGTGCGGGTGGGGTCCGGCGACCTGTCGGTGCGCATCCCG
GAGGGCGGCCGCGATGAACTGGGTCGCCTGGTCCGGGCCTTCAACCAGATGACGGAGAGACTCCGGGTTCAGGGCGAGAG
CCTGCGGGCCGAGCGGGACCTCCTGGACACCGCGGTTTCCGCCATGGGGGCGGGTCTGGCCCTGATCGACGCCCGCGGCC
GCATTCTCTGGGCAAACCGGCATCTGCTGGAGTGGTTCCCCGACGCCCGTGTGGGCGGCCCCTGCATGGCCGTCCTGGGT
CGCGCCTGCACCGGGGGCAGATGTGCGCCTGACGGCGAGTACCGGGTGCGCATCGGCGGCCGGGAGCGGGTCCTGCGGTA
TCAGAACCACCGGATCGCCGGCGCGGGCCCCGACGGACCGGCACGGCTCGAGGTGATCGAGGACGTGACCGAGCGGAGGG
CCATGGAGCAGATGGTCCGGCAGTCCGAGAAACTCGCCGCAGTGGGGCAGCTTGCGGCGGGCGTGGCCCACGAGATCAAC
AACCCGCTTGCGGTCATCTCGGCCTACGCGGAGGACCTGAGCGACCGCCTCCGCGACGAGGGGGCCGCGGCGCTGGAGCG
CGAGGGCGAGCTGGCGGCCTATCTGGCCCAGTTGCAGTTCCAGGTGCAGCGCTGCAAGGCGATTACCGGAAACCTGCTGG
ACTTCGCCCGGCAGGGCTCGGCCGACCCTGAGCCGGTCGACGTGGCGGGCGTTGCCCGGGCGACGGCCGCCTTGGTCGCG
CCCCGCGCCCGGCGGGCGGGGGTCGCGGTGGCGGTCGATGTTCCCGTTGCGCTGCCGGCGGTTCGGGCCAGCCGCGATCA
GCTGCAGCAGGTGTTTCTGAACCTGGTTACCAACGCCCTGGACGCCATCGAGGAAGGCGGGGGCGGGCGCGTGTGGGTCA
CGGCGGAGGCGGTCGCGGGCGCCCCGGCTTCAGAAGGGCCGGGCGTCTCGGACATCGGTGTCGCCGCGGGCGCAGGAGAG
GTCACCCGACCGGAGAGCGTGGTGCGGGTCGGCGTGCATGACGACGGGCCTGGAATGGACGAGGACACTCTTGCGAGGGC
GCTGGATCCGTTCTTCACCACCAAGCCGCCGGGACGCGGGACGGGGCTTGGGCTGTCCACCTGCTACGGCATCATCACGG
GCCTGGGGGGGCGGTTCTGGTTGGAGAGCCGCCGCGGACAGGGTACCCGGGTCTGGTTCGAACTGCCGGTCTGGGAGGGG
GAGTGA

Upstream 100 bases:

>100_bases
TCACGCTGAAGCCCGAGGGAATGGCGTTCGCCCACGTGCGGGGCGACGAGGCTGGCCCCGGCCCGGGCGATGTGCAGGTA
CCCGCCGGGGAGGTGGGGCC

Downstream 100 bases:

>100_bases
CGCGTGCGCACCTACGCCCTGCTGATCGCCGACGATGAGGCCTCGTTTCGCGAACTCCTGCAGCGGCGACTGGGCCGCAA
GGGTTACCTGGTGAAGGCCG

Product: two-component hybrid sensor and regulator

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTRWFGHLRLKVLAFGIAMSVLPVSIYGWYGLAAARTAQVEVVQAQNQAAARTVSEELARFVERITGQLQLLARLEGAGL
VSAPAAEQERVLYTLLRDTPYLEEVSLVDASGAEVSRASRREVVAGQPAARYSGEPYWADLRRGESVVGPVTFDPHGRPL
FTVGVPLPDGAGALIARPTLRGLVDNLAAVRAGGAVRTHVIDETGRLIGDSDFSLVLAGYRWAGVPAPDQPYGSLTGEEA
FGYLAEVPGLGWRVLAETTVDDAMVPVRRLAWEFAGGALLMVSVVVGLSIVFGLQLTVPLERLEAGAVRVGSGDLSVRIP
EGGRDELGRLVRAFNQMTERLRVQGESLRAERDLLDTAVSAMGAGLALIDARGRILWANRHLLEWFPDARVGGPCMAVLG
RACTGGRCAPDGEYRVRIGGRERVLRYQNHRIAGAGPDGPARLEVIEDVTERRAMEQMVRQSEKLAAVGQLAAGVAHEIN
NPLAVISAYAEDLSDRLRDEGAAALEREGELAAYLAQLQFQVQRCKAITGNLLDFARQGSADPEPVDVAGVARATAALVA
PRARRAGVAVAVDVPVALPAVRASRDQLQQVFLNLVTNALDAIEEGGGGRVWVTAEAVAGAPASEGPGVSDIGVAAGAGE
VTRPESVVRVGVHDDGPGMDEDTLARALDPFFTTKPPGRGTGLGLSTCYGIITGLGGRFWLESRRGQGTRVWFELPVWEG
E

Sequences:

>Translated_721_residues
MTRWFGHLRLKVLAFGIAMSVLPVSIYGWYGLAAARTAQVEVVQAQNQAAARTVSEELARFVERITGQLQLLARLEGAGL
VSAPAAEQERVLYTLLRDTPYLEEVSLVDASGAEVSRASRREVVAGQPAARYSGEPYWADLRRGESVVGPVTFDPHGRPL
FTVGVPLPDGAGALIARPTLRGLVDNLAAVRAGGAVRTHVIDETGRLIGDSDFSLVLAGYRWAGVPAPDQPYGSLTGEEA
FGYLAEVPGLGWRVLAETTVDDAMVPVRRLAWEFAGGALLMVSVVVGLSIVFGLQLTVPLERLEAGAVRVGSGDLSVRIP
EGGRDELGRLVRAFNQMTERLRVQGESLRAERDLLDTAVSAMGAGLALIDARGRILWANRHLLEWFPDARVGGPCMAVLG
RACTGGRCAPDGEYRVRIGGRERVLRYQNHRIAGAGPDGPARLEVIEDVTERRAMEQMVRQSEKLAAVGQLAAGVAHEIN
NPLAVISAYAEDLSDRLRDEGAAALEREGELAAYLAQLQFQVQRCKAITGNLLDFARQGSADPEPVDVAGVARATAALVA
PRARRAGVAVAVDVPVALPAVRASRDQLQQVFLNLVTNALDAIEEGGGGRVWVTAEAVAGAPASEGPGVSDIGVAAGAGE
VTRPESVVRVGVHDDGPGMDEDTLARALDPFFTTKPPGRGTGLGLSTCYGIITGLGGRFWLESRRGQGTRVWFELPVWEG
E
>Mature_720_residues
TRWFGHLRLKVLAFGIAMSVLPVSIYGWYGLAAARTAQVEVVQAQNQAAARTVSEELARFVERITGQLQLLARLEGAGLV
SAPAAEQERVLYTLLRDTPYLEEVSLVDASGAEVSRASRREVVAGQPAARYSGEPYWADLRRGESVVGPVTFDPHGRPLF
TVGVPLPDGAGALIARPTLRGLVDNLAAVRAGGAVRTHVIDETGRLIGDSDFSLVLAGYRWAGVPAPDQPYGSLTGEEAF
GYLAEVPGLGWRVLAETTVDDAMVPVRRLAWEFAGGALLMVSVVVGLSIVFGLQLTVPLERLEAGAVRVGSGDLSVRIPE
GGRDELGRLVRAFNQMTERLRVQGESLRAERDLLDTAVSAMGAGLALIDARGRILWANRHLLEWFPDARVGGPCMAVLGR
ACTGGRCAPDGEYRVRIGGRERVLRYQNHRIAGAGPDGPARLEVIEDVTERRAMEQMVRQSEKLAAVGQLAAGVAHEINN
PLAVISAYAEDLSDRLRDEGAAALEREGELAAYLAQLQFQVQRCKAITGNLLDFARQGSADPEPVDVAGVARATAALVAP
RARRAGVAVAVDVPVALPAVRASRDQLQQVFLNLVTNALDAIEEGGGGRVWVTAEAVAGAPASEGPGVSDIGVAAGAGEV
TRPESVVRVGVHDDGPGMDEDTLARALDPFFTTKPPGRGTGLGLSTCYGIITGLGGRFWLESRRGQGTRVWFELPVWEGE

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=412, Percent_Identity=24.5145631067961, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1790436, Length=266, Percent_Identity=31.203007518797, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790300, Length=374, Percent_Identity=26.2032085561497, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76697; Mature: 76566

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRWFGHLRLKVLAFGIAMSVLPVSIYGWYGLAAARTAQVEVVQAQNQAAARTVSEELAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEHHHHHHHHHHHHHHHH
FVERITGQLQLLARLEGAGLVSAPAAEQERVLYTLLRDTPYLEEVSLVDASGAEVSRASR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHH
REVVAGQPAARYSGEPYWADLRRGESVVGPVTFDPHGRPLFTVGVPLPDGAGALIARPTL
HHHHCCCCCHHCCCCCCHHHHHCCCCEECCEEECCCCCEEEEEECCCCCCCCCEEECHHH
RGLVDNLAAVRAGGAVRTHVIDETGRLIGDSDFSLVLAGYRWAGVPAPDQPYGSLTGEEA
HHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCEEEEECCEECCCCCCCCCCCCCCCHHH
FGYLAEVPGLGWRVLAETTVDDAMVPVRRLAWEFAGGALLMVSVVVGLSIVFGLQLTVPL
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCH
ERLEAGAVRVGSGDLSVRIPEGGRDELGRLVRAFNQMTERLRVQGESLRAERDLLDTAVS
HHHCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AMGAGLALIDARGRILWANRHLLEWFPDARVGGPCMAVLGRACTGGRCAPDGEYRVRIGG
HHCCCEEEEECCCCEEEECCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECC
RERVLRYQNHRIAGAGPDGPARLEVIEDVTERRAMEQMVRQSEKLAAVGQLAAGVAHEIN
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPLAVISAYAEDLSDRLRDEGAAALEREGELAAYLAQLQFQVQRCKAITGNLLDFARQGS
CCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADPEPVDVAGVARATAALVAPRARRAGVAVAVDVPVALPAVRASRDQLQQVFLNLVTNAL
CCCCCCCHHHHHHHHHHHHCCCHHHCCEEEEEECCHHCHHHHCCHHHHHHHHHHHHHHHH
DAIEEGGGGRVWVTAEAVAGAPASEGPGVSDIGVAAGAGEVTRPESVVRVGVHDDGPGMD
HHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
EDTLARALDPFFTTKPPGRGTGLGLSTCYGIITGLGGRFWLESRRGQGTRVWFELPVWEG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCC
E
C
>Mature Secondary Structure 
TRWFGHLRLKVLAFGIAMSVLPVSIYGWYGLAAARTAQVEVVQAQNQAAARTVSEELAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEHHHHHHHHHHHHHHHH
FVERITGQLQLLARLEGAGLVSAPAAEQERVLYTLLRDTPYLEEVSLVDASGAEVSRASR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHH
REVVAGQPAARYSGEPYWADLRRGESVVGPVTFDPHGRPLFTVGVPLPDGAGALIARPTL
HHHHCCCCCHHCCCCCCHHHHHCCCCEECCEEECCCCCEEEEEECCCCCCCCCEEECHHH
RGLVDNLAAVRAGGAVRTHVIDETGRLIGDSDFSLVLAGYRWAGVPAPDQPYGSLTGEEA
HHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCEEEEECCEECCCCCCCCCCCCCCCHHH
FGYLAEVPGLGWRVLAETTVDDAMVPVRRLAWEFAGGALLMVSVVVGLSIVFGLQLTVPL
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCH
ERLEAGAVRVGSGDLSVRIPEGGRDELGRLVRAFNQMTERLRVQGESLRAERDLLDTAVS
HHHCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AMGAGLALIDARGRILWANRHLLEWFPDARVGGPCMAVLGRACTGGRCAPDGEYRVRIGG
HHCCCEEEEECCCCEEEECCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECC
RERVLRYQNHRIAGAGPDGPARLEVIEDVTERRAMEQMVRQSEKLAAVGQLAAGVAHEIN
HHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPLAVISAYAEDLSDRLRDEGAAALEREGELAAYLAQLQFQVQRCKAITGNLLDFARQGS
CCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADPEPVDVAGVARATAALVAPRARRAGVAVAVDVPVALPAVRASRDQLQQVFLNLVTNAL
CCCCCCCHHHHHHHHHHHHCCCHHHCCEEEEEECCHHCHHHHCCHHHHHHHHHHHHHHHH
DAIEEGGGGRVWVTAEAVAGAPASEGPGVSDIGVAAGAGEVTRPESVVRVGVHDDGPGMD
HHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
EDTLARALDPFFTTKPPGRGTGLGLSTCYGIITGLGGRFWLESRRGQGTRVWFELPVWEG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]