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The map label for this gene is pilR [H]

Identifier: 51893225

GI number: 51893225

Start: 2225941

End: 2227335

Strand: Reverse

Name: pilR [H]

Synonym: STH2087

Alternate gene names: 51893225

Gene position: 2227335-2225941 (Counterclockwise)

Preceding gene: 51893226

Following gene: 51893223

Centisome position: 62.46

GC content: 68.82

Gene sequence:

>1395_bases
GTGCGCACCTACGCCCTGCTGATCGCCGACGATGAGGCCTCGTTTCGCGAACTCCTGCAGCGGCGACTGGGCCGCAAGGG
TTACCTGGTGAAGGCCGTGGGGTCGGGAACGGAGGCGCTGGAAGCCCTGGCGGAGGAGGAGTTCGACGCCGCCATCTTCG
ACATGAAGATGCCGGGGCTGGATGGCATCGCAACGCTCCGGCGGGCGCGGGAGATCCAGCCTTCCCTTCCCGTGCTGATC
CTCACCGGACACGGTTCCATCGAGACGGCGGTGGAGGCGATCCGGGCCGGCGCTTACGACTACCTGACCAAGCCGTGCAA
CCTGGCCGAGCTGGAGGCCGTCCTGGAGAAGGCGCTGGAGCGGCGTGTTCTGGAGGTGGAGAACCAGGGGCTGCGGGAGG
CGCTGCGGCGCCTGGGCGACGAGGTCGAGATCGTGGGCCGCAGCGAGCCGATCCGGCGGCTGTTGGAGCTGACCCGGCGG
GTGGCCCAGGGGTCCATGCCGGTGCTCATCGAGGGCGAGAGCGGCACGGGCAAGGAGCTGATCGCAAGGGCGGTCCACCT
GTGGAGTCCCCGGGCCTCGGGTCCGTTTATTCCCGTAAACGCCGGGGCGCTGCCGGCCCCGCTCCTGGAGAGCGAGCTGT
TCGGCCACGCGCGCGGCGCCTTCACCGGTGCGGTGGCGGCCAAACCGGGTTTGGTGGAGCTCGCCGAGGGTGGGACGCTG
TTCCTCGACGAGATCGGGGAGATGCCCCTGGAGGTGCAGGCCAAGCTGCTCCGTTTTCTGGAGAGCCATGAGGTGCGCCG
GGTAGGCGAAACGCGCATCCGGCGGGTGGACGTGCGCATCGTCGCCGCCACCAACCGGTGCCTGACCGATGAGGTGAACG
CGGGGCGGTTCCGGGAGGACCTGTACTACCGCCTGAACGTGGTCACCCTCCACGTGCCTCCCCTGCGGGAGCGGCGCGAG
GATATCCCGCTGCTGGTCGAACACTTCCTGGCCCGGCTGGGGTCGGCGAAGGAGATGACGCCGGACGCCATGGAGATCCT
CATGCAGCAGGAGTTCCGTGGGAATGTGCGAGAATTGTTCAACGTGGTGCAGCGCGGTGTGATCCTGAGTCCCGACCGGT
TTATCGCGCCGGAAGACCTGGGGCTTGCCGTGCGTCGCGCACGTTCCGGGCAGGCCGGCGCCGGCTTGGAAGCCGGGCCA
ACCGGGCGGCTGGATGGTTCCGCCAGCGAGACCGGTGAACCTTTCCCCACGCTGGACGAAGTGGAACGGGCGCACATCGC
CCGGGCGCTGGAGCGCACGGGCTGGAACCGGGCGCAGGCCGCACAGTTGCTCGGGGTCAGCGTACGCACGCTGTATCGGA
AGATCGAGAGCTACGGACTCAGGTCCGGGATGTAG

Upstream 100 bases:

>100_bases
CATCATCACGGGCCTGGGGGGGCGGTTCTGGTTGGAGAGCCGCCGCGGACAGGGTACCCGGGTCTGGTTCGAACTGCCGG
TCTGGGAGGGGGAGTGACGC

Downstream 100 bases:

>100_bases
TGGAGGGATTGAAGGTGCCGTGGGAGCGGCGCCTGGCTTATGTACTGCTCATCGTTGTGCTGGTCAGTCTGGGCATGATG
GTGGAGCGGGCGCCGCTGGA

Product: sigma-54-dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRTYALLIADDEASFRELLQRRLGRKGYLVKAVGSGTEALEALAEEEFDAAIFDMKMPGLDGIATLRRAREIQPSLPVLI
LTGHGSIETAVEAIRAGAYDYLTKPCNLAELEAVLEKALERRVLEVENQGLREALRRLGDEVEIVGRSEPIRRLLELTRR
VAQGSMPVLIEGESGTGKELIARAVHLWSPRASGPFIPVNAGALPAPLLESELFGHARGAFTGAVAAKPGLVELAEGGTL
FLDEIGEMPLEVQAKLLRFLESHEVRRVGETRIRRVDVRIVAATNRCLTDEVNAGRFREDLYYRLNVVTLHVPPLRERRE
DIPLLVEHFLARLGSAKEMTPDAMEILMQQEFRGNVRELFNVVQRGVILSPDRFIAPEDLGLAVRRARSGQAGAGLEAGP
TGRLDGSASETGEPFPTLDEVERAHIARALERTGWNRAQAAQLLGVSVRTLYRKIESYGLRSGM

Sequences:

>Translated_464_residues
MRTYALLIADDEASFRELLQRRLGRKGYLVKAVGSGTEALEALAEEEFDAAIFDMKMPGLDGIATLRRAREIQPSLPVLI
LTGHGSIETAVEAIRAGAYDYLTKPCNLAELEAVLEKALERRVLEVENQGLREALRRLGDEVEIVGRSEPIRRLLELTRR
VAQGSMPVLIEGESGTGKELIARAVHLWSPRASGPFIPVNAGALPAPLLESELFGHARGAFTGAVAAKPGLVELAEGGTL
FLDEIGEMPLEVQAKLLRFLESHEVRRVGETRIRRVDVRIVAATNRCLTDEVNAGRFREDLYYRLNVVTLHVPPLRERRE
DIPLLVEHFLARLGSAKEMTPDAMEILMQQEFRGNVRELFNVVQRGVILSPDRFIAPEDLGLAVRRARSGQAGAGLEAGP
TGRLDGSASETGEPFPTLDEVERAHIARALERTGWNRAQAAQLLGVSVRTLYRKIESYGLRSGM
>Mature_464_residues
MRTYALLIADDEASFRELLQRRLGRKGYLVKAVGSGTEALEALAEEEFDAAIFDMKMPGLDGIATLRRAREIQPSLPVLI
LTGHGSIETAVEAIRAGAYDYLTKPCNLAELEAVLEKALERRVLEVENQGLREALRRLGDEVEIVGRSEPIRRLLELTRR
VAQGSMPVLIEGESGTGKELIARAVHLWSPRASGPFIPVNAGALPAPLLESELFGHARGAFTGAVAAKPGLVELAEGGTL
FLDEIGEMPLEVQAKLLRFLESHEVRRVGETRIRRVDVRIVAATNRCLTDEVNAGRFREDLYYRLNVVTLHVPPLRERRE
DIPLLVEHFLARLGSAKEMTPDAMEILMQQEFRGNVRELFNVVQRGVILSPDRFIAPEDLGLAVRRARSGQAGAGLEAGP
TGRLDGSASETGEPFPTLDEVERAHIARALERTGWNRAQAAQLLGVSVRTLYRKIESYGLRSGM

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=44.5175438596491, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=42.1739130434783, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=39.1578947368421, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1788905, Length=376, Percent_Identity=47.3404255319149, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI1789233, Length=337, Percent_Identity=43.620178041543, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082117, Length=351, Percent_Identity=42.4501424501425, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1789087, Length=258, Percent_Identity=50.7751937984496, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI87082152, Length=327, Percent_Identity=40.9785932721713, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1787583, Length=323, Percent_Identity=38.0804953560372, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87081872, Length=333, Percent_Identity=39.3393393393393, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786524, Length=355, Percent_Identity=38.5915492957746, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=33.2278481012658, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=38.5593220338983, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=31.4516129032258, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789402, Length=124, Percent_Identity=35.4838709677419, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787375, Length=126, Percent_Identity=37.3015873015873, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=33.6206896551724, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693157, Length=116, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082012, Length=111, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51154; Mature: 51154

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTYALLIADDEASFRELLQRRLGRKGYLVKAVGSGTEALEALAEEEFDAAIFDMKMPGL
CCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCHHHEEECCCCC
DGIATLRRAREIQPSLPVLILTGHGSIETAVEAIRAGAYDYLTKPCNLAELEAVLEKALE
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
RRVLEVENQGLREALRRLGDEVEIVGRSEPIRRLLELTRRVAQGSMPVLIEGESGTGKEL
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
IARAVHLWSPRASGPFIPVNAGALPAPLLESELFGHARGAFTGAVAAKPGLVELAEGGTL
HHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEE
FLDEIGEMPLEVQAKLLRFLESHEVRRVGETRIRRVDVRIVAATNRCLTDEVNAGRFRED
EHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHCCCCCCCHHHHH
LYYRLNVVTLHVPPLRERREDIPLLVEHFLARLGSAKEMTPDAMEILMQQEFRGNVRELF
HEEEEEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHH
NVVQRGVILSPDRFIAPEDLGLAVRRARSGQAGAGLEAGPTGRLDGSASETGEPFPTLDE
HHHHCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VERAHIARALERTGWNRAQAAQLLGVSVRTLYRKIESYGLRSGM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRTYALLIADDEASFRELLQRRLGRKGYLVKAVGSGTEALEALAEEEFDAAIFDMKMPGL
CCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCHHHEEECCCCC
DGIATLRRAREIQPSLPVLILTGHGSIETAVEAIRAGAYDYLTKPCNLAELEAVLEKALE
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
RRVLEVENQGLREALRRLGDEVEIVGRSEPIRRLLELTRRVAQGSMPVLIEGESGTGKEL
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
IARAVHLWSPRASGPFIPVNAGALPAPLLESELFGHARGAFTGAVAAKPGLVELAEGGTL
HHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEE
FLDEIGEMPLEVQAKLLRFLESHEVRRVGETRIRRVDVRIVAATNRCLTDEVNAGRFRED
EHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHCCCCCCCHHHHH
LYYRLNVVTLHVPPLRERREDIPLLVEHFLARLGSAKEMTPDAMEILMQQEFRGNVRELF
HEEEEEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHH
NVVQRGVILSPDRFIAPEDLGLAVRRARSGQAGAGLEAGPTGRLDGSASETGEPFPTLDE
HHHHCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VERAHIARALERTGWNRAQAAQLLGVSVRTLYRKIESYGLRSGM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]