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The map label for this gene is leuB [H]

Identifier: 51893084

GI number: 51893084

Start: 2087035

End: 2088108

Strand: Reverse

Name: leuB [H]

Synonym: STH1946

Alternate gene names: 51893084

Gene position: 2088108-2087035 (Counterclockwise)

Preceding gene: 51893085

Following gene: 51893083

Centisome position: 58.55

GC content: 70.2

Gene sequence:

>1074_bases
ATGGCATTCGTTCCGACCATCGTGGTACTGGAAGGGGACCAGACCGGGCAGGAGCTGCTGGAAGAGGCCGTTCGGCTTCT
CAGCCCTGACGTGATCGGCTTGCCGCTGCATCTGGTGCGTTATGACCTGAGCCTGGAGAACCGCCGGGCGACCAGTAACC
GGGTCGTCTACGAGGCGGCCGCGGCGATGCGGGAGCACGGGTACGGACTGAAGGCGGCGACCATCACCCCGGAGGGCCGG
GGAGACGTGGGGTCGCCCAACGCCATCCTGCGGCGGGAGATCGACGGCACGGTCATCCTGCGGACCGGCCGGCCCCTGCC
GGGGGTCGAGACCATCGGCGGAATCACCGCGCCCATCGCGGTGGTGCGCATGGCGACCGAGGATGCGTACGAGGCGAAGG
AGTGGCGCGAGGGCGAGGGCGATGAGGAGCGGGCGTTCCGCACCACCTACATCTCCGCCCGCAACTGCCGTGCCACCGCC
GAGTTCGCCTTCCGCCTCGCGCGGCAGATGGGGGCGCTGGTCTTCGGCGGCCCCAAGTGGACGGTGTCGCCGACCTACGA
GGGACTGCTGAAGGAGGCGATGGACGAGGCGGCCAGGCGGAATCCGGACGTGCCCTACGACCCGCAGCTCATCGACGCAG
CGTACGCCCTGCTGATCGCGCGGGCGACCCGGCCGCTGGTCATCCCGTGCCTCAACCGGGACGGGGACATCCTGTCTGAC
CTGGTGCTGGCGCTCTACGGCTCCATCGCGGGATCGGAGTCGCTGCTCATCGCCTTCGACGAACAGTTCAACCCGCGGGT
GGTCATGGCCGAGGCGCCGCACGGCACCGCGCCCAGCCTGCAGGGCAAGAACCTGGCCAACCCGCTGGCGATGCAGCTTG
CGGCGGGCGCTCTGCTGAAGCAGATGCCGGATCCGGAGTACCAGCGTGCGGGCGCGGCGATCCAGGAAGCCTGCCTGCAG
GCTGTGGCGCAGGGCGTGCGGACCGCCGACCTGGGCGGAACCGCCCGGACCACCGAGTTCACCGACGAGGTGATCCGCCG
GGTGAAGGAGAAGCTGGCGGTCCCGGCCCGCTGA

Upstream 100 bases:

>100_bases
GCATAAGAGAAATAGAACATCGTTCTGTACCGTGGAACGATTGGGGCTTAACAGGGGGCGTCTCACCGATTAGGATTGTG
ACGGAAGGGGGCTGAAGCAC

Downstream 100 bases:

>100_bases
GCCCGCGCACCCCGCCGCGCCGGCCGGAATAGGCTGGTGAGGGCGAGGGGGCAGCGGCCATGTTCGTCACCCACGGCGAC
ACGATCGTATTCCGCTCGGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MAFVPTIVVLEGDQTGQELLEEAVRLLSPDVIGLPLHLVRYDLSLENRRATSNRVVYEAAAAMREHGYGLKAATITPEGR
GDVGSPNAILRREIDGTVILRTGRPLPGVETIGGITAPIAVVRMATEDAYEAKEWREGEGDEERAFRTTYISARNCRATA
EFAFRLARQMGALVFGGPKWTVSPTYEGLLKEAMDEAARRNPDVPYDPQLIDAAYALLIARATRPLVIPCLNRDGDILSD
LVLALYGSIAGSESLLIAFDEQFNPRVVMAEAPHGTAPSLQGKNLANPLAMQLAAGALLKQMPDPEYQRAGAAIQEACLQ
AVAQGVRTADLGGTARTTEFTDEVIRRVKEKLAVPAR

Sequences:

>Translated_357_residues
MAFVPTIVVLEGDQTGQELLEEAVRLLSPDVIGLPLHLVRYDLSLENRRATSNRVVYEAAAAMREHGYGLKAATITPEGR
GDVGSPNAILRREIDGTVILRTGRPLPGVETIGGITAPIAVVRMATEDAYEAKEWREGEGDEERAFRTTYISARNCRATA
EFAFRLARQMGALVFGGPKWTVSPTYEGLLKEAMDEAARRNPDVPYDPQLIDAAYALLIARATRPLVIPCLNRDGDILSD
LVLALYGSIAGSESLLIAFDEQFNPRVVMAEAPHGTAPSLQGKNLANPLAMQLAAGALLKQMPDPEYQRAGAAIQEACLQ
AVAQGVRTADLGGTARTTEFTDEVIRRVKEKLAVPAR
>Mature_356_residues
AFVPTIVVLEGDQTGQELLEEAVRLLSPDVIGLPLHLVRYDLSLENRRATSNRVVYEAAAAMREHGYGLKAATITPEGRG
DVGSPNAILRREIDGTVILRTGRPLPGVETIGGITAPIAVVRMATEDAYEAKEWREGEGDEERAFRTTYISARNCRATAE
FAFRLARQMGALVFGGPKWTVSPTYEGLLKEAMDEAARRNPDVPYDPQLIDAAYALLIARATRPLVIPCLNRDGDILSDL
VLALYGSIAGSESLLIAFDEQFNPRVVMAEAPHGTAPSLQGKNLANPLAMQLAAGALLKQMPDPEYQRAGAAIQEACLQA
VAQGVRTADLGGTARTTEFTDEVIRRVKEKLAVPAR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=354, Percent_Identity=27.683615819209, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI28178825, Length=413, Percent_Identity=26.634382566586, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI28178832, Length=380, Percent_Identity=24.4736842105263, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4758582, Length=267, Percent_Identity=28.0898876404494, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI28178821, Length=349, Percent_Identity=25.214899713467, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI28178816, Length=343, Percent_Identity=25.3644314868805, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788101, Length=373, Percent_Identity=27.3458445040214, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081683, Length=374, Percent_Identity=30.2139037433155, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=290, Percent_Identity=30.6896551724138, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17540874, Length=414, Percent_Identity=26.5700483091787, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=25.2840909090909, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=24.5014245014245, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324709, Length=350, Percent_Identity=27.4285714285714, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320137, Length=413, Percent_Identity=27.1186440677966, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=27.2980501392758, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324291, Length=351, Percent_Identity=25.0712250712251, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319830, Length=371, Percent_Identity=29.3800539083558, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643268, Length=292, Percent_Identity=27.0547945205479, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24643270, Length=292, Percent_Identity=27.0547945205479, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=27.0194986072423, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281362242, Length=356, Percent_Identity=27.5280898876405, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648872, Length=356, Percent_Identity=27.5280898876405, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161078639, Length=295, Percent_Identity=23.728813559322, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161078633, Length=295, Percent_Identity=23.728813559322, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650122, Length=295, Percent_Identity=23.728813559322, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161078637, Length=295, Percent_Identity=23.728813559322, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI161078635, Length=295, Percent_Identity=23.728813559322, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38632; Mature: 38500

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFVPTIVVLEGDQTGQELLEEAVRLLSPDVIGLPLHLVRYDLSLENRRATSNRVVYEAA
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
AAMREHGYGLKAATITPEGRGDVGSPNAILRREIDGTVILRTGRPLPGVETIGGITAPIA
HHHHHCCCCCEEEEECCCCCCCCCCCCHHHEEECCCEEEEECCCCCCCHHHHCCHHHHHH
VVRMATEDAYEAKEWREGEGDEERAFRTTYISARNCRATAEFAFRLARQMGALVFGGPKW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCEEECCCCE
TVSPTYEGLLKEAMDEAARRNPDVPYDPQLIDAAYALLIARATRPLVIPCLNRDGDILSD
EECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
LVLALYGSIAGSESLLIAFDEQFNPRVVMAEAPHGTAPSLQGKNLANPLAMQLAAGALLK
HHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QMPDPEYQRAGAAIQEACLQAVAQGVRTADLGGTARTTEFTDEVIRRVKEKLAVPAR
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AFVPTIVVLEGDQTGQELLEEAVRLLSPDVIGLPLHLVRYDLSLENRRATSNRVVYEAA
CCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHH
AAMREHGYGLKAATITPEGRGDVGSPNAILRREIDGTVILRTGRPLPGVETIGGITAPIA
HHHHHCCCCCEEEEECCCCCCCCCCCCHHHEEECCCEEEEECCCCCCCHHHHCCHHHHHH
VVRMATEDAYEAKEWREGEGDEERAFRTTYISARNCRATAEFAFRLARQMGALVFGGPKW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCEEECCCCE
TVSPTYEGLLKEAMDEAARRNPDVPYDPQLIDAAYALLIARATRPLVIPCLNRDGDILSD
EECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
LVLALYGSIAGSESLLIAFDEQFNPRVVMAEAPHGTAPSLQGKNLANPLAMQLAAGALLK
HHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QMPDPEYQRAGAAIQEACLQAVAQGVRTADLGGTARTTEFTDEVIRRVKEKLAVPAR
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]