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The map label for this gene is spoIVB [H]

Identifier: 51892971

GI number: 51892971

Start: 1979309

End: 1980718

Strand: Reverse

Name: spoIVB [H]

Synonym: STH1833

Alternate gene names: 51892971

Gene position: 1980718-1979309 (Counterclockwise)

Preceding gene: 51892972

Following gene: 51892970

Centisome position: 55.54

GC content: 69.01

Gene sequence:

>1410_bases
ATGTCAACCCGGGAGAAGTGGCGGAGGGCCGCCGGCCTTCTGCTGGTCGTCCTGATCCTTTCCGTCTGTGCCACACCCGA
GTTCCGCACCTTTGTTTCCCTGCCCGATCACCTGCGCCTTCCCCAGGGCCAGGCCCACGAACTCTCGCTGGGCATCCCCT
TCAGCACGGCCGTGCCCACCTCGGGCCAGGGGCTCGCGGTGCAGCGGTCCGGGTCCCCGCTGCAGCTGGCCGCCGAGCAG
ACCGGCCGGTACACCTTGGATGTGAAGCTGTTCGGGCTGATCCCGATCCGGCGGATGACGGTGGACGTCGTCCCCGAGAT
CCGGGTGGTGCCCGGAGGCCATTCGATCGGCGTGCGGCTGCAGAGCGACGGGGTGCAGGTGGTGGGGTTCGCGGCCGTCG
AGGATGAAGAGGGGCGCGTCCACCACCCGGGGCGGGCGGCCGGCCTGCAGGTCGGCGACACCATCCTCGCCATTGACGGG
CGGCCCGTGCGCGGCGAGGACCACGCAGTTCACCTGTTTCAGGAAGCCGGCCGGCGGGGCCGGGAGATCACCCTCACCGT
CCGCCGGGGCGACGAGCGGCTGGAGCGGACCGTGAAGCCCGTGCGGGAAAAGGAAACGGGCCGCTGGCGGGTCGGGCTCT
ACATCCGCGACGGCGCCTCGGGCGTCGGAACGCTCACCTTCTACCACCCGCCCACCCGCAAGTTCGGCGCCCTCGGCCAC
GTGATCGCGGAGGGCGAGAGCCGGCGGCCGTTCCAGTTCGCCCACGGGCAGATCACCGCGGCCGACGTCGTGAAGATCCA
GAAGGGGAGGCGGGCCGCGCCGGGCGAGAAGATCACCAGCCACCCCGACTCCGGCGGCCGGCTGGGCGTCATCGAGAAGA
ACACGCCGTTTGGCATCTTCGGCCGGCTGAACAGCCCCCTCAAGAACCCGCTTTACGAGGAACCACTGCCGGTGGCCATG
GCCAGCCAGGTCAAGGAGGGGCCGGCCGAGATGCTCACCGTCGTCGACGGGCAGAAGATCGAGCGGTTCCAAGTCGAGAT
CGTCCGCCTGATCCGCCAGCCCACCGCTGAAGGGAAGAACATGATCGTGCAGGTCACGGATCCCCGGCTGCTGGAAGCGA
CCGGCGGCATCGTGCAGGGGATGTCGGGCAGTCCCATCATCCAGGACGGCCGGGTGGTCGGGGCCGTGACCCACGTCTTC
GTCAACGACCCGACGCGCGGATACGGCGTGCTGATCGAGTGGATGCTGCAGGAGGCCGGCCTCCTGCCCGCCGAGCGCAA
TACGGAACCGAACCAGTCGGGCCTCCGGGAGGACACGCTCCTCCCGGAGGTTTTGTCTGGGCGGGCGGTCCGCGGCGGTG
GCAGGCGAGCTGGTAACGTTTGTGTAACATCTATGTGTGTATACTTATAA

Upstream 100 bases:

>100_bases
GCGCCGCCGTTTTCTTAGAGACACAGAGACAGAGAAGTGGGAGACGCAGTGGAGAAGGGGTCCGCCCTGGGGAAGAGAAG
CTTCAGGGAGGGACGCCCCG

Downstream 100 bases:

>100_bases
ACTATCGGTTCCCGGTTGCGCCTTTTGTGGGTGAGCGGCCCCGGGAACGTACGGTCAGGTTGGGGGTTGAATGAGAATGT
CGGCCCACAAAGTTCGGCTC

Product: stage IV sporulation protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSTREKWRRAAGLLLVVLILSVCATPEFRTFVSLPDHLRLPQGQAHELSLGIPFSTAVPTSGQGLAVQRSGSPLQLAAEQ
TGRYTLDVKLFGLIPIRRMTVDVVPEIRVVPGGHSIGVRLQSDGVQVVGFAAVEDEEGRVHHPGRAAGLQVGDTILAIDG
RPVRGEDHAVHLFQEAGRRGREITLTVRRGDERLERTVKPVREKETGRWRVGLYIRDGASGVGTLTFYHPPTRKFGALGH
VIAEGESRRPFQFAHGQITAADVVKIQKGRRAAPGEKITSHPDSGGRLGVIEKNTPFGIFGRLNSPLKNPLYEEPLPVAM
ASQVKEGPAEMLTVVDGQKIERFQVEIVRLIRQPTAEGKNMIVQVTDPRLLEATGGIVQGMSGSPIIQDGRVVGAVTHVF
VNDPTRGYGVLIEWMLQEAGLLPAERNTEPNQSGLREDTLLPEVLSGRAVRGGGRRAGNVCVTSMCVYL

Sequences:

>Translated_469_residues
MSTREKWRRAAGLLLVVLILSVCATPEFRTFVSLPDHLRLPQGQAHELSLGIPFSTAVPTSGQGLAVQRSGSPLQLAAEQ
TGRYTLDVKLFGLIPIRRMTVDVVPEIRVVPGGHSIGVRLQSDGVQVVGFAAVEDEEGRVHHPGRAAGLQVGDTILAIDG
RPVRGEDHAVHLFQEAGRRGREITLTVRRGDERLERTVKPVREKETGRWRVGLYIRDGASGVGTLTFYHPPTRKFGALGH
VIAEGESRRPFQFAHGQITAADVVKIQKGRRAAPGEKITSHPDSGGRLGVIEKNTPFGIFGRLNSPLKNPLYEEPLPVAM
ASQVKEGPAEMLTVVDGQKIERFQVEIVRLIRQPTAEGKNMIVQVTDPRLLEATGGIVQGMSGSPIIQDGRVVGAVTHVF
VNDPTRGYGVLIEWMLQEAGLLPAERNTEPNQSGLREDTLLPEVLSGRAVRGGGRRAGNVCVTSMCVYL
>Mature_468_residues
STREKWRRAAGLLLVVLILSVCATPEFRTFVSLPDHLRLPQGQAHELSLGIPFSTAVPTSGQGLAVQRSGSPLQLAAEQT
GRYTLDVKLFGLIPIRRMTVDVVPEIRVVPGGHSIGVRLQSDGVQVVGFAAVEDEEGRVHHPGRAAGLQVGDTILAIDGR
PVRGEDHAVHLFQEAGRRGREITLTVRRGDERLERTVKPVREKETGRWRVGLYIRDGASGVGTLTFYHPPTRKFGALGHV
IAEGESRRPFQFAHGQITAADVVKIQKGRRAAPGEKITSHPDSGGRLGVIEKNTPFGIFGRLNSPLKNPLYEEPLPVAMA
SQVKEGPAEMLTVVDGQKIERFQVEIVRLIRQPTAEGKNMIVQVTDPRLLEATGGIVQGMSGSPIIQDGRVVGAVTHVFV
NDPTRGYGVLIEWMLQEAGLLPAERNTEPNQSGLREDTLLPEVLSGRAVRGGGRRAGNVCVTSMCVYL

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 50917; Mature: 50786

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTREKWRRAAGLLLVVLILSVCATPEFRTFVSLPDHLRLPQGQAHELSLGIPFSTAVPT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHCCCCCCCCCEEEECCCCCCCCCC
SGQGLAVQRSGSPLQLAAEQTGRYTLDVKLFGLIPIRRMTVDVVPEIRVVPGGHSIGVRL
CCCCEEEEECCCCEEEEECCCCCEEEEEEEEEEEEEEHEEEEECCCEEEECCCCEEEEEE
QSDGVQVVGFAAVEDEEGRVHHPGRAAGLQVGDTILAIDGRPVRGEDHAVHLFQEAGRRG
CCCCEEEEEEEEEECCCCCEECCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHCCCCC
REITLTVRRGDERLERTVKPVREKETGRWRVGLYIRDGASGVGTLTFYHPPTRKFGALGH
CEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCHHHHCHHH
VIAEGESRRPFQFAHGQITAADVVKIQKGRRAAPGEKITSHPDSGGRLGVIEKNTPFGIF
HEECCCCCCCEEECCCEEEHHHHHEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEE
GRLNSPLKNPLYEEPLPVAMASQVKEGPAEMLTVVDGQKIERFQVEIVRLIRQPTAEGKN
ECCCCCCCCCCCCCCCCHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCE
MIVQVTDPRLLEATGGIVQGMSGSPIIQDGRVVGAVTHVFVNDPTRGYGVLIEWMLQEAG
EEEEECCCCEEECCCCCEECCCCCCEEECCEEEEEEEEEEECCCCCCHHHHHHHHHHHCC
LLPAERNTEPNQSGLREDTLLPEVLSGRAVRGGGRRAGNVCVTSMCVYL
CCCCCCCCCCCCCCCCHHHCCHHHHCCCEECCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
STREKWRRAAGLLLVVLILSVCATPEFRTFVSLPDHLRLPQGQAHELSLGIPFSTAVPT
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHCCCCCCCCCEEEECCCCCCCCCC
SGQGLAVQRSGSPLQLAAEQTGRYTLDVKLFGLIPIRRMTVDVVPEIRVVPGGHSIGVRL
CCCCEEEEECCCCEEEEECCCCCEEEEEEEEEEEEEEHEEEEECCCEEEECCCCEEEEEE
QSDGVQVVGFAAVEDEEGRVHHPGRAAGLQVGDTILAIDGRPVRGEDHAVHLFQEAGRRG
CCCCEEEEEEEEEECCCCCEECCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHCCCCC
REITLTVRRGDERLERTVKPVREKETGRWRVGLYIRDGASGVGTLTFYHPPTRKFGALGH
CEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCHHHHCHHH
VIAEGESRRPFQFAHGQITAADVVKIQKGRRAAPGEKITSHPDSGGRLGVIEKNTPFGIF
HEECCCCCCCEEECCCEEEHHHHHEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEE
GRLNSPLKNPLYEEPLPVAMASQVKEGPAEMLTVVDGQKIERFQVEIVRLIRQPTAEGKN
ECCCCCCCCCCCCCCCCHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCE
MIVQVTDPRLLEATGGIVQGMSGSPIIQDGRVVGAVTHVFVNDPTRGYGVLIEWMLQEAG
EEEEECCCCEEECCCCCEECCCCCCEEECCEEEEEEEEEEECCCCCCHHHHHHHHHHHCC
LLPAERNTEPNQSGLREDTLLPEVLSGRAVRGGGRRAGNVCVTSMCVYL
CCCCCCCCCCCCCCCCHHHCCHHHHCCCEECCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]