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The map label for this gene is dacF [H]

Identifier: 51892953

GI number: 51892953

Start: 1958778

End: 1959989

Strand: Reverse

Name: dacF [H]

Synonym: STH1815

Alternate gene names: 51892953

Gene position: 1959989-1958778 (Counterclockwise)

Preceding gene: 51892956

Following gene: 51892952

Centisome position: 54.96

GC content: 68.23

Gene sequence:

>1212_bases
ATGGAGCGCTGGCCGCCGGCGCCTGACGTCGAGGACGGCGACCGGCTGCTGCAAGGCGGCGGGGCCTACGGCCTGGACGA
GCCGGTCGAGGCGGCTGCCGCGGCGGAAGAGGCCGGCAGCGGCGAACCGGTGAAGATCGAGTCGCCGTCCGCGATCCTGA
TGGAACCCCTGAGCGGACGGGTCATCTACCAGAAGAACGCCCACGAGCGGCGCAACCCGGCCAGCGTCACCAAGATCATG
ACCCTGATCGTCGCCTTCGACGCCATCCGGGATGGCAAGGTGAGCCTGAAGGACCCGGTCACCATCTCGGAGGAGGCCAA
GCGGCAGCCGGGAACCACCATCTTCGCCGACGTGGGCGAGACCTTCTCGCTGGAGGAGTTGCTGCTCTCGGTGGCGGTGG
GGTCGGCCAACGACGCCGCGGTGGCGGTGGCAGAGCACGTGGCCGGGTCTGTGGACGCGTTCGTGGCCCTCATGAACAAG
AAGGCGAAGGAGCTGGGGATGAACGACACCCAGTTCATGAACCCCACGGGGCTCTCGGCCGAGGGGCATTACAGCACGGC
CTATGACATCGCCCTCATGAGCCGCCACGCCGTGCTGCACTACCCCGAGCTGGTGAAGCTGACCGCGATCTACGGCGCGG
AGCTCAACGTGCCGTGGCGCAAGAACGGGCCGAAGTTCCAGCTGTGGAACAACAACAAGCTGCTCACGTGGTACCCGGGG
GCGGACGGCCTGAAGACGGGCTGGACCCAGGCGGCCGGATACTGCCTCGCGGCGACGGCGGTGCAGAACAACACCCGCAT
GATCGCCGTCGTCATGGGCGCGGAGAGCGCGAAACAGCGGAACATCGAGGTCGCCCGGCTGCTGGACTACGGCTTCGCCA
ACTATACGGCCGTGGAGATCGCCCCTGCGGACAAGAAGTTCGGCCCCGTGCGGGTGGCCCGCGGGGCGGTGGCTGCCATC
GAACCGGTGCCGCGGGCGGCGTTCGGGGTCAGCGTGCCCAAGGGCTCCCAGGACAAGGTGAAGTGGGAGGTGCGGCTGCC
GAAACAGGTCGCGGCGCCCCTGGAGAAGGGCCAGGCGGTGGGGGAGATCGTCGCCACGCTGGACGGGCAGGAGGTCGGCC
GCATGGAGCTGGTCGCCCCCGAGGCGGTCGGAAAGGCCAACCTGTGGCAGTCCGTGGTGCACACGGTACGGAACATCTTC
AGCTTTGATTGA

Upstream 100 bases:

>100_bases
CGAGGAGGCATGACCCATGTGGAGAACGCGCACGCGCTGCTGGGCGGCGCTGCTGTGCGCCGTCCTCTGGGTGGCGGCGC
TTTCGCCGGCCGCCCGGGGA

Downstream 100 bases:

>100_bases
GCGAATGGTCTCGGGCCCGCCGGCCGTCAGGTGCCGGCGGGCCTGTTCGCGCGTATTTGGTCGAACAATGTCGACCGAAA
GATGGGGAAGAAGGCAGGAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MERWPPAPDVEDGDRLLQGGGAYGLDEPVEAAAAAEEAGSGEPVKIESPSAILMEPLSGRVIYQKNAHERRNPASVTKIM
TLIVAFDAIRDGKVSLKDPVTISEEAKRQPGTTIFADVGETFSLEELLLSVAVGSANDAAVAVAEHVAGSVDAFVALMNK
KAKELGMNDTQFMNPTGLSAEGHYSTAYDIALMSRHAVLHYPELVKLTAIYGAELNVPWRKNGPKFQLWNNNKLLTWYPG
ADGLKTGWTQAAGYCLAATAVQNNTRMIAVVMGAESAKQRNIEVARLLDYGFANYTAVEIAPADKKFGPVRVARGAVAAI
EPVPRAAFGVSVPKGSQDKVKWEVRLPKQVAAPLEKGQAVGEIVATLDGQEVGRMELVAPEAVGKANLWQSVVHTVRNIF
SFD

Sequences:

>Translated_403_residues
MERWPPAPDVEDGDRLLQGGGAYGLDEPVEAAAAAEEAGSGEPVKIESPSAILMEPLSGRVIYQKNAHERRNPASVTKIM
TLIVAFDAIRDGKVSLKDPVTISEEAKRQPGTTIFADVGETFSLEELLLSVAVGSANDAAVAVAEHVAGSVDAFVALMNK
KAKELGMNDTQFMNPTGLSAEGHYSTAYDIALMSRHAVLHYPELVKLTAIYGAELNVPWRKNGPKFQLWNNNKLLTWYPG
ADGLKTGWTQAAGYCLAATAVQNNTRMIAVVMGAESAKQRNIEVARLLDYGFANYTAVEIAPADKKFGPVRVARGAVAAI
EPVPRAAFGVSVPKGSQDKVKWEVRLPKQVAAPLEKGQAVGEIVATLDGQEVGRMELVAPEAVGKANLWQSVVHTVRNIF
SFD
>Mature_403_residues
MERWPPAPDVEDGDRLLQGGGAYGLDEPVEAAAAAEEAGSGEPVKIESPSAILMEPLSGRVIYQKNAHERRNPASVTKIM
TLIVAFDAIRDGKVSLKDPVTISEEAKRQPGTTIFADVGETFSLEELLLSVAVGSANDAAVAVAEHVAGSVDAFVALMNK
KAKELGMNDTQFMNPTGLSAEGHYSTAYDIALMSRHAVLHYPELVKLTAIYGAELNVPWRKNGPKFQLWNNNKLLTWYPG
ADGLKTGWTQAAGYCLAATAVQNNTRMIAVVMGAESAKQRNIEVARLLDYGFANYTAVEIAPADKKFGPVRVARGAVAAI
EPVPRAAFGVSVPKGSQDKVKWEVRLPKQVAAPLEKGQAVGEIVATLDGQEVGRMELVAPEAVGKANLWQSVVHTVRNIF
SFD

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=362, Percent_Identity=36.1878453038674, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787062, Length=359, Percent_Identity=34.2618384401114, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786851, Length=374, Percent_Identity=33.4224598930481, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082054, Length=250, Percent_Identity=32.4, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43217; Mature: 43217

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERWPPAPDVEDGDRLLQGGGAYGLDEPVEAAAAAEEAGSGEPVKIESPSAILMEPLSGR
CCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCE
VIYQKNAHERRNPASVTKIMTLIVAFDAIRDGKVSLKDPVTISEEAKRQPGTTIFADVGE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEECHHHHCCCCCEEEECCCC
TFSLEELLLSVAVGSANDAAVAVAEHVAGSVDAFVALMNKKAKELGMNDTQFMNPTGLSA
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EGHYSTAYDIALMSRHAVLHYPELVKLTAIYGAELNVPWRKNGPKFQLWNNNKLLTWYPG
CCCCCHHHHHHHHHCCHHCCCCHHEEEEEEECCEECCCCCCCCCEEEEECCCEEEEECCC
ADGLKTGWTQAAGYCLAATAVQNNTRMIAVVMGAESAKQRNIEVARLLDYGFANYTAVEI
CCCCCCCHHHHCCEEEEEEEECCCCEEEEEEECCCCHHHCCCHHHHHHHCCCCCEEEEEE
APADKKFGPVRVARGAVAAIEPVPRAAFGVSVPKGSQDKVKWEVRLPKQVAAPLEKGQAV
ECCCCCCCCCHHHCCCEEECCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHCHHH
GEIVATLDGQEVGRMELVAPEAVGKANLWQSVVHTVRNIFSFD
HHHHHHCCCCCCCCEEEECCHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERWPPAPDVEDGDRLLQGGGAYGLDEPVEAAAAAEEAGSGEPVKIESPSAILMEPLSGR
CCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCE
VIYQKNAHERRNPASVTKIMTLIVAFDAIRDGKVSLKDPVTISEEAKRQPGTTIFADVGE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEECHHHHCCCCCEEEECCCC
TFSLEELLLSVAVGSANDAAVAVAEHVAGSVDAFVALMNKKAKELGMNDTQFMNPTGLSA
CCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EGHYSTAYDIALMSRHAVLHYPELVKLTAIYGAELNVPWRKNGPKFQLWNNNKLLTWYPG
CCCCCHHHHHHHHHCCHHCCCCHHEEEEEEECCEECCCCCCCCCEEEEECCCEEEEECCC
ADGLKTGWTQAAGYCLAATAVQNNTRMIAVVMGAESAKQRNIEVARLLDYGFANYTAVEI
CCCCCCCHHHHCCEEEEEEEECCCCEEEEEEECCCCHHHCCCHHHHHHHCCCCCEEEEEE
APADKKFGPVRVARGAVAAIEPVPRAAFGVSVPKGSQDKVKWEVRLPKQVAAPLEKGQAV
ECCCCCCCCCHHHCCCEEECCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHCHHH
GEIVATLDGQEVGRMELVAPEAVGKANLWQSVVHTVRNIFSFD
HHHHHHCCCCCCCCEEEECCHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]