Click here to switch to the map view.

The map label for this gene is rluB [H]

Identifier: 51892921

GI number: 51892921

Start: 1927371

End: 1928135

Strand: Reverse

Name: rluB [H]

Synonym: STH1783

Alternate gene names: 51892921

Gene position: 1928135-1927371 (Counterclockwise)

Preceding gene: 51892923

Following gene: 51892920

Centisome position: 54.07

GC content: 70.85

Gene sequence:

>765_bases
GTGGCCGAGCGGCTGCAGAAGGTGATGGCCCGGGCCGGCGTGGCTTCGCGCCGCCACTGTGAGGCCCTGATCCAGGCCGG
TCGGGTGACGGTCAACGGCCAGGTCGTCACGCAGCTGGGCACGAAGGTGGTCCCCGGCAAGGACGTGATCGAGGTGGACG
GCCGCCCGCTCGGCACGCCCGAGACCAAGGTGTACGTGATGCTGAACAAGCCGAAGGGCTACGTGTCGACGCTCTACGAC
CCCCAGGGGCGGCCCAAGGTGATCGACCTGCTGGGCGACGAGGTGGCCCAGCGGGTCTATCCGGTCGGCCGGCTCGACTT
TGACACGGAGGGGCTGCTGCTCCTCACCAACGATGGCGACCTGGCCCACGGCCTGATGCACCCGTCCCGCATGGTGAAGA
AGACTTACATCGTCAAGGTGCGCGGCGTGCCCGGTCCGGCGAAGCTGCGGGCCCTGCAGGAGGGAATCGAGCTGGAGGAC
GGCCCGACGGCTCCGGCCGAGGTAAAGATGCTGGACGTGCGCCCGCCCAACAGCGCGACCCTGTCGGTGCGCATCCACGA
GGGGCGCAACCGGCAGGTGCGGCGCATGTTCGCGGCGCTGGGGCACGAGGTGATCCACCTGCGCCGGACGACCCTGGGGC
CGCTGAAGCTCGGCGACCTTTCCGTGGGCGAGTGGCGTTACCTGACCGACCAGGAGGTAGCGGACCTGTACCGGGCGGCC
GGGCTCAGGCTGGGCGGCAGGCCCCGCCGCGCGGGCCGCAGGTAG

Upstream 100 bases:

>100_bases
CGGCCTGACAGGACGGCGCAGCCCGCGCAAGTCGAAGCACGAGGCCGGCGGGGGCCGCAAGGCCGCCGGAGGCCGCCGGC
TCACCGCCGCCGAGGCCGAG

Downstream 100 bases:

>100_bases
GGGACGGACGCGAGGACAGAAGAGGAAAAGGCAGGGCTCTCTCCGAAATGTGGTAAGGGGAGCGCCTTCCTGTGTCTGAA
GGTCAGGGCGGCGTTCTCCC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MAERLQKVMARAGVASRRHCEALIQAGRVTVNGQVVTQLGTKVVPGKDVIEVDGRPLGTPETKVYVMLNKPKGYVSTLYD
PQGRPKVIDLLGDEVAQRVYPVGRLDFDTEGLLLLTNDGDLAHGLMHPSRMVKKTYIVKVRGVPGPAKLRALQEGIELED
GPTAPAEVKMLDVRPPNSATLSVRIHEGRNRQVRRMFAALGHEVIHLRRTTLGPLKLGDLSVGEWRYLTDQEVADLYRAA
GLRLGGRPRRAGRR

Sequences:

>Translated_254_residues
MAERLQKVMARAGVASRRHCEALIQAGRVTVNGQVVTQLGTKVVPGKDVIEVDGRPLGTPETKVYVMLNKPKGYVSTLYD
PQGRPKVIDLLGDEVAQRVYPVGRLDFDTEGLLLLTNDGDLAHGLMHPSRMVKKTYIVKVRGVPGPAKLRALQEGIELED
GPTAPAEVKMLDVRPPNSATLSVRIHEGRNRQVRRMFAALGHEVIHLRRTTLGPLKLGDLSVGEWRYLTDQEVADLYRAA
GLRLGGRPRRAGRR
>Mature_253_residues
AERLQKVMARAGVASRRHCEALIQAGRVTVNGQVVTQLGTKVVPGKDVIEVDGRPLGTPETKVYVMLNKPKGYVSTLYDP
QGRPKVIDLLGDEVAQRVYPVGRLDFDTEGLLLLTNDGDLAHGLMHPSRMVKKTYIVKVRGVPGPAKLRALQEGIELEDG
PTAPAEVKMLDVRPPNSATLSVRIHEGRNRQVRRMFAALGHEVIHLRRTTLGPLKLGDLSVGEWRYLTDQEVADLYRAAG
LRLGGRPRRAGRR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=223, Percent_Identity=44.3946188340807, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=38.5593220338983, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=35.0210970464135, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=38.5474860335196, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28013; Mature: 27882

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLQKVMARAGVASRRHCEALIQAGRVTVNGQVVTQLGTKVVPGKDVIEVDGRPLGTP
CHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCEEEEECCCEECCCCCEEEECCCCCCCC
ETKVYVMLNKPKGYVSTLYDPQGRPKVIDLLGDEVAQRVYPVGRLDFDTEGLLLLTNDGD
CCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
LAHGLMHPSRMVKKTYIVKVRGVPGPAKLRALQEGIELEDGPTAPAEVKMLDVRPPNSAT
HHHHHCCHHHHHCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCE
LSVRIHEGRNRQVRRMFAALGHEVIHLRRTTLGPLKLGDLSVGEWRYLTDQEVADLYRAA
EEEEEECCCCHHHHHHHHHHHHHHEEHHHCCCCCEEECCCCCCCEEECCHHHHHHHHHHH
GLRLGGRPRRAGRR
CCCCCCCCCCCCCC
>Mature Secondary Structure 
AERLQKVMARAGVASRRHCEALIQAGRVTVNGQVVTQLGTKVVPGKDVIEVDGRPLGTP
HHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCEEEEECCCEECCCCCEEEECCCCCCCC
ETKVYVMLNKPKGYVSTLYDPQGRPKVIDLLGDEVAQRVYPVGRLDFDTEGLLLLTNDGD
CCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
LAHGLMHPSRMVKKTYIVKVRGVPGPAKLRALQEGIELEDGPTAPAEVKMLDVRPPNSAT
HHHHHCCHHHHHCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCE
LSVRIHEGRNRQVRRMFAALGHEVIHLRRTTLGPLKLGDLSVGEWRYLTDQEVADLYRAA
EEEEEECCCCHHHHHHHHHHHHHHEEHHHCCCCCEEECCCCCCCEEECCHHHHHHHHHHH
GLRLGGRPRRAGRR
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]