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The map label for this gene is gadB [C]

Identifier: 51892412

GI number: 51892412

Start: 1410857

End: 1412380

Strand: Reverse

Name: gadB [C]

Synonym: STH1274

Alternate gene names: 51892412

Gene position: 1412380-1410857 (Counterclockwise)

Preceding gene: 51892421

Following gene: 51892411

Centisome position: 39.61

GC content: 70.54

Gene sequence:

>1524_bases
GTGCTTTCGGCGTTTTCCCCGCCTTTGCCCTGCGACCCGGCGCGTTCCCACCCAACGCCGGAATTTCCCTCCTCATTGCA
GGACTATTGCGAAATTCGCGGAATTCAATCGCAACCGCCTGCGAGGAGGGACCCCACCATGGACTGGCTCGCCTCCCTGC
GGTCGCAGATCAAGCCTTACCGCGACCGCTTTCCTTCCCACGCCCGCCTGCCACGGGCCGGCCTGCCCCGCGCCGAGATC
CTCGCCGAGATCGCGGCCATGGGCGCGGCCGAGTCCCCGGCCTGGCGGGACGGCTATGCCTCCGGCGCCGTCTACCACGG
CGATGAGCACCACATCGCGTTCCTCAACGAGGTCTACGCCCTCCAGTCCCAGTCCAACCCGCTCCACCCGGACCTCTGGC
CGAGCACGGCCAAGTTCGAGGCCGAGGTCGTCGCCATGACCGCCCACATGCTGGGCGGGGACGCCGCCGGCGGGACCGTC
TGCGGCACCGTGACCTCCGGCGGCACGGAGTCGCTCCTCCTGGCCATGAAGACCTACCGGGACTGGGCCCGCGCGACCAA
GGGGATCACCGCCCCGGAGGCCGTGGTGCCGGTGAGCGCGCACGCCGCCTTCGACAAGGCCGCGCAGTACTTCGGCATCA
AGCTGGTCCGCACCCCGCTGGACGCCGATTACCGGGCAGACGTCGCGGCCATGCGGGAAGCCATTACCCCCAACACCGTG
GTCGTCGCCGGATCCGCGCCGGGCTACCCCCACGGCGTCGTCGACCCCATTCCCGAGATCGCCGCCCTGGCCGCGGAACA
CGGCATCGGCTGCCATGTGGACGCCTGCCTGGGCGGGTTCATCCTGCCGTGGGCGGAGCGGCTGGGCTACCCGGTGCCCC
CCTTCGATTTCCGGCTGGAGGGCGTCACCTCCGTCTCGGCCGACACCCACAAGTACGGCTACGGGGCCAAGGGAACGTCG
GTGATCCTCTACCGCAGACCTGATCTGCTGCACTACCAGTACTTCATCGCGGCCGACTGGCCCGGAGGCCTCTACTTCTC
CCCCACCTTCGCCGGCAGCCGGCCCGGCGCGCTCAGCGCGACCGCGTGGGCCGCCATGCTCTCCCTGGGGGAGGAGGGCT
ACCTGGATGCCACCCGCCGGATCCTGCAAGCCGCCGACCGGCTTAAGGCGGGCGTGCGCGCGATCCCCAGCCTGAAGATC
CTGGGCGACCCGCTGTGGGTGATCGCCGTGGCCTCGGACGAGCTCAACATCTACCAGGTGATGGAGGAGATGGCCGGGCG
GGGCTGGCGGCTGAACGGACTGCACCGGCCGCCGGCGTTTCACGTCGCCCTGACGCTTCGCCACACCGAGCCCGGGGTGG
TCGACCGGTTCCTCGCCGACCTGCAGGACGCGGTGGCTCAGGTCCGGGCCCACCCCGAAAAGGCGACCGGGATGGCCCCG
GTCTACGGCATGGCCGCCGCCGCGCCGCCGGAGCTGGTCCGGCAGGTGCTCACCGGCTTCATCGACCTGCTGTACGAGGT
GTGA

Upstream 100 bases:

>100_bases
TGTACCCGCCGGAAGCGGGCCCGAACCCATTCCCGGTCCTCGCCCGTGTGGTGGGCGAGGACCCGGCACCTCAGCCACCC
CTGTTCGTCCTCCGGTCTGT

Downstream 100 bases:

>100_bases
CCGCCATGGCCCAGCAGTACGTCCTGGCGCTGGATCTGGGCACGTCGGCCCTGAAGGCGGCCCTGGTCTCCTTCACCGGC
GCCGTCGCCGGCCTGGAGAC

Product: putative sphingosine-1-phosphate lyase

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLSAFSPPLPCDPARSHPTPEFPSSLQDYCEIRGIQSQPPARRDPTMDWLASLRSQIKPYRDRFPSHARLPRAGLPRAEI
LAEIAAMGAAESPAWRDGYASGAVYHGDEHHIAFLNEVYALQSQSNPLHPDLWPSTAKFEAEVVAMTAHMLGGDAAGGTV
CGTVTSGGTESLLLAMKTYRDWARATKGITAPEAVVPVSAHAAFDKAAQYFGIKLVRTPLDADYRADVAAMREAITPNTV
VVAGSAPGYPHGVVDPIPEIAALAAEHGIGCHVDACLGGFILPWAERLGYPVPPFDFRLEGVTSVSADTHKYGYGAKGTS
VILYRRPDLLHYQYFIAADWPGGLYFSPTFAGSRPGALSATAWAAMLSLGEEGYLDATRRILQAADRLKAGVRAIPSLKI
LGDPLWVIAVASDELNIYQVMEEMAGRGWRLNGLHRPPAFHVALTLRHTEPGVVDRFLADLQDAVAQVRAHPEKATGMAP
VYGMAAAAPPELVRQVLTGFIDLLYEV

Sequences:

>Translated_507_residues
MLSAFSPPLPCDPARSHPTPEFPSSLQDYCEIRGIQSQPPARRDPTMDWLASLRSQIKPYRDRFPSHARLPRAGLPRAEI
LAEIAAMGAAESPAWRDGYASGAVYHGDEHHIAFLNEVYALQSQSNPLHPDLWPSTAKFEAEVVAMTAHMLGGDAAGGTV
CGTVTSGGTESLLLAMKTYRDWARATKGITAPEAVVPVSAHAAFDKAAQYFGIKLVRTPLDADYRADVAAMREAITPNTV
VVAGSAPGYPHGVVDPIPEIAALAAEHGIGCHVDACLGGFILPWAERLGYPVPPFDFRLEGVTSVSADTHKYGYGAKGTS
VILYRRPDLLHYQYFIAADWPGGLYFSPTFAGSRPGALSATAWAAMLSLGEEGYLDATRRILQAADRLKAGVRAIPSLKI
LGDPLWVIAVASDELNIYQVMEEMAGRGWRLNGLHRPPAFHVALTLRHTEPGVVDRFLADLQDAVAQVRAHPEKATGMAP
VYGMAAAAPPELVRQVLTGFIDLLYEV
>Mature_507_residues
MLSAFSPPLPCDPARSHPTPEFPSSLQDYCEIRGIQSQPPARRDPTMDWLASLRSQIKPYRDRFPSHARLPRAGLPRAEI
LAEIAAMGAAESPAWRDGYASGAVYHGDEHHIAFLNEVYALQSQSNPLHPDLWPSTAKFEAEVVAMTAHMLGGDAAGGTV
CGTVTSGGTESLLLAMKTYRDWARATKGITAPEAVVPVSAHAAFDKAAQYFGIKLVRTPLDADYRADVAAMREAITPNTV
VVAGSAPGYPHGVVDPIPEIAALAAEHGIGCHVDACLGGFILPWAERLGYPVPPFDFRLEGVTSVSADTHKYGYGAKGTS
VILYRRPDLLHYQYFIAADWPGGLYFSPTFAGSRPGALSATAWAAMLSLGEEGYLDATRRILQAADRLKAGVRAIPSLKI
LGDPLWVIAVASDELNIYQVMEEMAGRGWRLNGLHRPPAFHVALTLRHTEPGVVDRFLADLQDAVAQVRAHPEKATGMAP
VYGMAAAAPPELVRQVLTGFIDLLYEV

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=477, Percent_Identity=45.2830188679245, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1787769, Length=398, Percent_Identity=25.1256281407035, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789934, Length=392, Percent_Identity=25.2551020408163, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543922, Length=477, Percent_Identity=40.6708595387841, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17557272, Length=445, Percent_Identity=39.5505617977528, Blast_Score=314, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI25148342, Length=380, Percent_Identity=40.2631578947368, Blast_Score=285, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6320500, Length=517, Percent_Identity=37.5241779497099, Blast_Score=332, Evalue=9e-92,
Organism=Drosophila melanogaster, GI21355963, Length=457, Percent_Identity=42.4507658643326, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654344, Length=457, Percent_Identity=42.4507658643326, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 54646; Mature: 54646

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAFSPPLPCDPARSHPTPEFPSSLQDYCEIRGIQSQPPARRDPTMDWLASLRSQIKPY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHH
RDRFPSHARLPRAGLPRAEILAEIAAMGAAESPAWRDGYASGAVYHGDEHHIAFLNEVYA
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHH
LQSQSNPLHPDLWPSTAKFEAEVVAMTAHMLGGDAAGGTVCGTVTSGGTESLLLAMKTYR
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHH
DWARATKGITAPEAVVPVSAHAAFDKAAQYFGIKLVRTPLDADYRADVAAMREAITPNTV
HHHHHHCCCCCCCCEECCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCE
VVAGSAPGYPHGVVDPIPEIAALAAEHGIGCHVDACLGGFILPWAERLGYPVPPFDFRLE
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCCCCCCEEEC
GVTSVSADTHKYGYGAKGTSVILYRRPDLLHYQYFIAADWPGGLYFSPTFAGSRPGALSA
CCEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEECCCCCCEECCCCCCCCCCCCHH
TAWAAMLSLGEEGYLDATRRILQAADRLKAGVRAIPSLKILGDPLWVIAVASDELNIYQV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCHHHHH
MEEMAGRGWRLNGLHRPPAFHVALTLRHTEPGVVDRFLADLQDAVAQVRAHPEKATGMAP
HHHHHCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCC
VYGMAAAAPPELVRQVLTGFIDLLYEV
HHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSAFSPPLPCDPARSHPTPEFPSSLQDYCEIRGIQSQPPARRDPTMDWLASLRSQIKPY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHH
RDRFPSHARLPRAGLPRAEILAEIAAMGAAESPAWRDGYASGAVYHGDEHHIAFLNEVYA
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHH
LQSQSNPLHPDLWPSTAKFEAEVVAMTAHMLGGDAAGGTVCGTVTSGGTESLLLAMKTYR
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHH
DWARATKGITAPEAVVPVSAHAAFDKAAQYFGIKLVRTPLDADYRADVAAMREAITPNTV
HHHHHHCCCCCCCCEECCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCE
VVAGSAPGYPHGVVDPIPEIAALAAEHGIGCHVDACLGGFILPWAERLGYPVPPFDFRLE
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCCCCCCEEEC
GVTSVSADTHKYGYGAKGTSVILYRRPDLLHYQYFIAADWPGGLYFSPTFAGSRPGALSA
CCEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEECCCCCCEECCCCCCCCCCCCHH
TAWAAMLSLGEEGYLDATRRILQAADRLKAGVRAIPSLKILGDPLWVIAVASDELNIYQV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCHHHHH
MEEMAGRGWRLNGLHRPPAFHVALTLRHTEPGVVDRFLADLQDAVAQVRAHPEKATGMAP
HHHHHCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCC
VYGMAAAAPPELVRQVLTGFIDLLYEV
HHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA