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The map label for this gene is cwhA [H]

Identifier: 51892206

GI number: 51892206

Start: 1180819

End: 1182348

Strand: Reverse

Name: cwhA [H]

Synonym: STH1068

Alternate gene names: 51892206

Gene position: 1182348-1180819 (Counterclockwise)

Preceding gene: 51892209

Following gene: 51892203

Centisome position: 33.15

GC content: 68.24

Gene sequence:

>1530_bases
ATGCGCAAGGGTCGAGGGCTGAGCTTCTTCGTGCTCCTGCTGTTCCTGTTGACCGTGGTGGGGACCGGATGGGCGGCCCC
TGAGGAGTTCGAGCGGGGCCACCGGCTGGACGGCCCCATGGGGCAGGCCTTCCGGAAGGCTGCCGACGCCTACGGCGTGC
CGGTGGAGCTGCTGGTGGCCCTGGCCTACGCCGAGACCCGGCTGGACGACCACGACGGCGTGCCCAGCGTCGCCGGGGGG
TACGGCGTGATGCACCTGGTCTCCAATCCCCAGGTGACGACGCTGGACCTGGCCGCCAAGCTCACGGGGCTCGACCCGGC
CCGGCTCAGGACCGACACCGCGGCCAACATCCTGGGCGGTGCGGCGCTGCTGGCCCACTACGCCGCGGAGCAGGGGGCGG
ACGTCAAATCCCTGAAGCTGGGCGACTGGTTTGAGGCGGTGGCCCGGTACAGCCAGCTCGAAGGGGTGGCCGCCAGGGAC
TACGCGCTGCAGGTCTACGAGTTTCTGAACCGGGGCATCGACGCCGTGGTCGACGGCGAGGAGATCCGCATCGAGCCGCA
GGCGGTCACGCCCGACCTGGGCCGGTTCGCCGGCCTGATCACCGTCCAGTCCCAGGACTACGGCCCCGCCGCCTGGGTGC
CCGCCCACTCCTCCAACTACACCGCCTCCAACCGGCCCAGCAGCTACCCCATCCGGTACATCGTCATCCACGTGACCCAG
GGCTCCTATTCCGGGACCATCAGCTGGTTCCAGAACCCCAGTTCCAACGTGAGCGCCCACTACGTCATCCGCTCCAGCGA
CGGCGCCATCACCCAGATGGTGCGGGAGAAGGACATCGCCTGGCACGCCGGCAACTGGACCTACAACACCCAGTCCATCG
GCATCGAACACGAAGGCTGGGTGTCCAGCTGCACCTGGTTCACCGACGCCATGTACCGGGCCAGCGCGGCGCTCACCCGC
AACATCGCCCAGAAGTACGGCATCCCCATGACCCGTAGCTACATCATCGGCCACAACGAGGTGCCGGGGGCCACCCACAC
CGACCCGGGCAGCTGCTGGGACTGGAACTACTACATGAGCCTGGTCACCCAGTCCTCGGGCTCGTGGTCGGCCATTGTGG
ACAACACCACCAGCGGCCGGTTCCGGGCCAGCAGCAACTGGGGCACCTCCTCCTGGTCGAGCCAGCGGTACGGCAGCAAC
TACCGCTTCGCCGAGCCGAAGGCGGTCAGCGACGCGGCCTACTACAAGTTCAACATCCCGTCCACCGGCAACTACGACAT
CTACATGTGGTACCCGGCCAACAGCGGCTACAACGACAGGACCCCGGTGGTGATCTGGCAGGCCAACAGCGCGGGCACGG
GCACCACGCCGGTGACGGTGTACGTCAACCAGCGACAAAACGGCGGCAGGTGGGTGCACCTGGGCACGTTCCGGCTGCTG
GCCGGCGACCGGGAGATCATCGCCGTGAGCCGCTGGACCAGCGGGACGGGCTACGTGATCGCCGACGCCTTCCGGGTGGT
GCGGCGGTAG

Upstream 100 bases:

>100_bases
ACTCGACCGATATCGACAATTTTCCTGTCAGAGTATTGATTGTTCTGACTTATTTGTGTAAAAAGTCCCTACCATCATAC
GATGGAAGGGGAGGTTCACC

Downstream 100 bases:

>100_bases
AACGATCGAGCCGGGGGTATCCCCCGGCTCGCGCCGCGTCCTCCTTCACTTTCCAAAGCGGGCGATGATCGCCTCGTGGG
CCCGCATCTCCAGGTCGTCG

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MRKGRGLSFFVLLLFLLTVVGTGWAAPEEFERGHRLDGPMGQAFRKAADAYGVPVELLVALAYAETRLDDHDGVPSVAGG
YGVMHLVSNPQVTTLDLAAKLTGLDPARLRTDTAANILGGAALLAHYAAEQGADVKSLKLGDWFEAVARYSQLEGVAARD
YALQVYEFLNRGIDAVVDGEEIRIEPQAVTPDLGRFAGLITVQSQDYGPAAWVPAHSSNYTASNRPSSYPIRYIVIHVTQ
GSYSGTISWFQNPSSNVSAHYVIRSSDGAITQMVREKDIAWHAGNWTYNTQSIGIEHEGWVSSCTWFTDAMYRASAALTR
NIAQKYGIPMTRSYIIGHNEVPGATHTDPGSCWDWNYYMSLVTQSSGSWSAIVDNTTSGRFRASSNWGTSSWSSQRYGSN
YRFAEPKAVSDAAYYKFNIPSTGNYDIYMWYPANSGYNDRTPVVIWQANSAGTGTTPVTVYVNQRQNGGRWVHLGTFRLL
AGDREIIAVSRWTSGTGYVIADAFRVVRR

Sequences:

>Translated_509_residues
MRKGRGLSFFVLLLFLLTVVGTGWAAPEEFERGHRLDGPMGQAFRKAADAYGVPVELLVALAYAETRLDDHDGVPSVAGG
YGVMHLVSNPQVTTLDLAAKLTGLDPARLRTDTAANILGGAALLAHYAAEQGADVKSLKLGDWFEAVARYSQLEGVAARD
YALQVYEFLNRGIDAVVDGEEIRIEPQAVTPDLGRFAGLITVQSQDYGPAAWVPAHSSNYTASNRPSSYPIRYIVIHVTQ
GSYSGTISWFQNPSSNVSAHYVIRSSDGAITQMVREKDIAWHAGNWTYNTQSIGIEHEGWVSSCTWFTDAMYRASAALTR
NIAQKYGIPMTRSYIIGHNEVPGATHTDPGSCWDWNYYMSLVTQSSGSWSAIVDNTTSGRFRASSNWGTSSWSSQRYGSN
YRFAEPKAVSDAAYYKFNIPSTGNYDIYMWYPANSGYNDRTPVVIWQANSAGTGTTPVTVYVNQRQNGGRWVHLGTFRLL
AGDREIIAVSRWTSGTGYVIADAFRVVRR
>Mature_509_residues
MRKGRGLSFFVLLLFLLTVVGTGWAAPEEFERGHRLDGPMGQAFRKAADAYGVPVELLVALAYAETRLDDHDGVPSVAGG
YGVMHLVSNPQVTTLDLAAKLTGLDPARLRTDTAANILGGAALLAHYAAEQGADVKSLKLGDWFEAVARYSQLEGVAARD
YALQVYEFLNRGIDAVVDGEEIRIEPQAVTPDLGRFAGLITVQSQDYGPAAWVPAHSSNYTASNRPSSYPIRYIVIHVTQ
GSYSGTISWFQNPSSNVSAHYVIRSSDGAITQMVREKDIAWHAGNWTYNTQSIGIEHEGWVSSCTWFTDAMYRASAALTR
NIAQKYGIPMTRSYIIGHNEVPGATHTDPGSCWDWNYYMSLVTQSSGSWSAIVDNTTSGRFRASSNWGTSSWSSQRYGSN
YRFAEPKAVSDAAYYKFNIPSTGNYDIYMWYPANSGYNDRTPVVIWQANSAGTGTTPVTVYVNQRQNGGRWVHLGTFRLL
AGDREIIAVSRWTSGTGYVIADAFRVVRR

Specific function: Antibacterial activity against Gram-positive bacteria M.luteus, S.aureus, E.faecalis and P.acidilactici and Gram- negative bacterium E.coli [H]

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786300, Length=136, Percent_Identity=36.0294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 55953; Mature: 55953

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGRGLSFFVLLLFLLTVVGTGWAAPEEFERGHRLDGPMGQAFRKAADAYGVPVELLVA
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
LAYAETRLDDHDGVPSVAGGYGVMHLVSNPQVTTLDLAAKLTGLDPARLRTDTAANILGG
HHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHCCCCHHHHCCCHHHHHHHH
AALLAHYAAEQGADVKSLKLGDWFEAVARYSQLEGVAARDYALQVYEFLNRGIDAVVDGE
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCC
EIRIEPQAVTPDLGRFAGLITVQSQDYGPAAWVPAHSSNYTASNRPSSYPIRYIVIHVTQ
EEEEECCCCCCCHHHHEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEEEC
GSYSGTISWFQNPSSNVSAHYVIRSSDGAITQMVREKDIAWHAGNWTYNTQSIGIEHEGW
CCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHCCCEEECCCEEECCCEECCCCCCC
VSSCTWFTDAMYRASAALTRNIAQKYGIPMTRSYIIGHNEVPGATHTDPGSCWDWNYYMS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEECHHEEEE
LVTQSSGSWSAIVDNTTSGRFRASSNWGTSSWSSQRYGSNYRFAEPKAVSDAAYYKFNIP
EEEECCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
STGNYDIYMWYPANSGYNDRTPVVIWQANSAGTGTTPVTVYVNQRQNGGRWVHLGTFRLL
CCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEEEEEEE
AGDREIIAVSRWTSGTGYVIADAFRVVRR
ECCCEEEEEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MRKGRGLSFFVLLLFLLTVVGTGWAAPEEFERGHRLDGPMGQAFRKAADAYGVPVELLVA
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
LAYAETRLDDHDGVPSVAGGYGVMHLVSNPQVTTLDLAAKLTGLDPARLRTDTAANILGG
HHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHCCCCHHHHCCCHHHHHHHH
AALLAHYAAEQGADVKSLKLGDWFEAVARYSQLEGVAARDYALQVYEFLNRGIDAVVDGE
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCC
EIRIEPQAVTPDLGRFAGLITVQSQDYGPAAWVPAHSSNYTASNRPSSYPIRYIVIHVTQ
EEEEECCCCCCCHHHHEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEEEC
GSYSGTISWFQNPSSNVSAHYVIRSSDGAITQMVREKDIAWHAGNWTYNTQSIGIEHEGW
CCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHCCCEEECCCEEECCCEECCCCCCC
VSSCTWFTDAMYRASAALTRNIAQKYGIPMTRSYIIGHNEVPGATHTDPGSCWDWNYYMS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEECHHEEEE
LVTQSSGSWSAIVDNTTSGRFRASSNWGTSSWSSQRYGSNYRFAEPKAVSDAAYYKFNIP
EEEECCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
STGNYDIYMWYPANSGYNDRTPVVIWQANSAGTGTTPVTVYVNQRQNGGRWVHLGTFRLL
CCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEEEEEEE
AGDREIIAVSRWTSGTGYVIADAFRVVRR
ECCCEEEEEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10833271 [H]