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The map label for this gene is glcF [H]

Identifier: 51892130

GI number: 51892130

Start: 1110309

End: 1111595

Strand: Reverse

Name: glcF [H]

Synonym: STH992

Alternate gene names: 51892130

Gene position: 1111595-1110309 (Counterclockwise)

Preceding gene: 51892131

Following gene: 51892128

Centisome position: 31.17

GC content: 70.78

Gene sequence:

>1287_bases
GTGAAGGTGCAGATCTCCGAGGATATGATCCTCAACTGCATGCGCTGCGGGTTCTGCCTGCCCGCCTGTCCCACCTACCG
GTACAAGCAGGTGGAGTCGGCGAGCCCGCGGGGCCGCATCGCGCTCATGCGCGCGGTGATGGAGGGCAAGCTGGACGTCC
TGGACATCGCCGGCCCCCTGGACACCTGCCTGGGCTGCCGAGCCTGCGAGCCCGCCTGCCCCGCGGGCGTCACCTACGGA
GCCATCCTGGAGCAGGGGCGGGCCCAGCTCAACCAGGTGAGGCCCCTGCCCGGCGTCGTGCGGTGGGCCTACCGCTGGCT
GCTGGGCACGCCGGGTGGCATCCGGTTCTCCGCCTGGGCGCTGTGGCTTTACCAGAAGCTCCGCCTGAACGCGCTGGCGC
ACCGGCTGAACCTGGTGGAGAAGATCGGCGGTAAGGGGCTGGCCGATATGGAGCGGGCCATCCCGCCCGTCGCCTCGCCG
GCCAGGCGCGCCGCCCGCAAGCCGGCATACCCCGCCGCCGGGAACCGCCGGTACCGGGTGGCCTTCTTCATGGGATGCCT
CTCCGACATCGTGTTCTTCGAGACCAATCAGAACGCGATCGAGGTCCTCACGGGGCTCGGGTGCGAGGTGGTGCTCCCCG
CGGGTCAGGGATGCTGCGGCGCCGTGCACAGCCACGCCGGCGAGCACGAGCTGGCCGTTGCGCAGGCCAAGCGGAACATC
GCCGCCTTCGAGACCAGCGGCTGCGACTTCATCGTGAGCACGGCCGGCGGCTGCGGCGCGGCCCTGCGGGAGTACGACAA
GCTGCTGGCCGACGACCCGGAGTGGGCCGACCGGGCCCGGGCGTTCTCGTCCCGCTGCCGGGACTTCTCCGAACTGGTGG
AGCAGCTCGGTCCCATCCCGATGGGGGACATGACCGGCACCTACACCTATCAGGATTCGTGCCACCTGCGCAACGTTCAG
AAGGTGGCGGCGCCGCCCCGCAACCTGCTCAAGTCCATGCCCGGCGCCCGTTTCGTCGAGCTGCCGGAGGCCGACCGCTG
CTGTGGCGCCGCGGGCACCTACATGATCACCCAGGCCCTGCTCTCCGACCGGATCCTGGACGACAAGGCGCAGAAGGTCG
CCCAGACCGGCGCCGCCACCCTGGTGGTGGCCAACACCCCCTGCCACCTGGAGATGCTGGAAGGCATCCAGCGGGCGGGC
CTGGCCGACCGGGTGCAGGTACGCCACATCGCCGACGTGGTCGCCGAGGCGATGCGCAAGGCCCCGGCTGCCGGGGGAAG
TCACTGA

Upstream 100 bases:

>100_bases
AGCTCATGAAGAACATCAAGAAAGCCTTCGATCCCCACGGCATCCTGAACCCGGGCAAGCTCTTCGCGGGCGAGACCCGG
AAGCGGGTGGTGGTCCGCCA

Downstream 100 bases:

>100_bases
GCGCAACGCGAAACGAAGAGGGCGGTTCCGCCTTGCCTGGCGGAACCGCCCTCCTTTCGCCTGCATGACAGACAGCCGCT
GCCGAAGCGCCGCGCACCGG

Product: glycolate oxidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKVQISEDMILNCMRCGFCLPACPTYRYKQVESASPRGRIALMRAVMEGKLDVLDIAGPLDTCLGCRACEPACPAGVTYG
AILEQGRAQLNQVRPLPGVVRWAYRWLLGTPGGIRFSAWALWLYQKLRLNALAHRLNLVEKIGGKGLADMERAIPPVASP
ARRAARKPAYPAAGNRRYRVAFFMGCLSDIVFFETNQNAIEVLTGLGCEVVLPAGQGCCGAVHSHAGEHELAVAQAKRNI
AAFETSGCDFIVSTAGGCGAALREYDKLLADDPEWADRARAFSSRCRDFSELVEQLGPIPMGDMTGTYTYQDSCHLRNVQ
KVAAPPRNLLKSMPGARFVELPEADRCCGAAGTYMITQALLSDRILDDKAQKVAQTGAATLVVANTPCHLEMLEGIQRAG
LADRVQVRHIADVVAEAMRKAPAAGGSH

Sequences:

>Translated_428_residues
MKVQISEDMILNCMRCGFCLPACPTYRYKQVESASPRGRIALMRAVMEGKLDVLDIAGPLDTCLGCRACEPACPAGVTYG
AILEQGRAQLNQVRPLPGVVRWAYRWLLGTPGGIRFSAWALWLYQKLRLNALAHRLNLVEKIGGKGLADMERAIPPVASP
ARRAARKPAYPAAGNRRYRVAFFMGCLSDIVFFETNQNAIEVLTGLGCEVVLPAGQGCCGAVHSHAGEHELAVAQAKRNI
AAFETSGCDFIVSTAGGCGAALREYDKLLADDPEWADRARAFSSRCRDFSELVEQLGPIPMGDMTGTYTYQDSCHLRNVQ
KVAAPPRNLLKSMPGARFVELPEADRCCGAAGTYMITQALLSDRILDDKAQKVAQTGAATLVVANTPCHLEMLEGIQRAG
LADRVQVRHIADVVAEAMRKAPAAGGSH
>Mature_428_residues
MKVQISEDMILNCMRCGFCLPACPTYRYKQVESASPRGRIALMRAVMEGKLDVLDIAGPLDTCLGCRACEPACPAGVTYG
AILEQGRAQLNQVRPLPGVVRWAYRWLLGTPGGIRFSAWALWLYQKLRLNALAHRLNLVEKIGGKGLADMERAIPPVASP
ARRAARKPAYPAAGNRRYRVAFFMGCLSDIVFFETNQNAIEVLTGLGCEVVLPAGQGCCGAVHSHAGEHELAVAQAKRNI
AAFETSGCDFIVSTAGGCGAALREYDKLLADDPEWADRARAFSSRCRDFSELVEQLGPIPMGDMTGTYTYQDSCHLRNVQ
KVAAPPRNLLKSMPGARFVELPEADRCCGAAGTYMITQALLSDRILDDKAQKVAQTGAATLVVANTPCHLEMLEGIQRAG
LADRVQVRHIADVVAEAMRKAPAAGGSH

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=440, Percent_Identity=31.1363636363636, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1786497, Length=238, Percent_Identity=33.6134453781513, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788576, Length=352, Percent_Identity=25.2840909090909, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46246; Mature: 46246

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQISEDMILNCMRCGFCLPACPTYRYKQVESASPRGRIALMRAVMEGKLDVLDIAGPL
CEEECCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCCHH
DTCLGCRACEPACPAGVTYGAILEQGRAQLNQVRPLPGVVRWAYRWLLGTPGGIRFSAWA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHH
LWLYQKLRLNALAHRLNLVEKIGGKGLADMERAIPPVASPARRAARKPAYPAAGNRRYRV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHH
AFFMGCLSDIVFFETNQNAIEVLTGLGCEVVLPAGQGCCGAVHSHAGEHELAVAQAKRNI
HHHHHHHHHHEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHCH
AAFETSGCDFIVSTAGGCGAALREYDKLLADDPEWADRARAFSSRCRDFSELVEQLGPIP
HEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
MGDMTGTYTYQDSCHLRNVQKVAAPPRNLLKSMPGARFVELPEADRCCGAAGTYMITQAL
CCCCCCCEEECCCHHHHHHHHHCCCHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHH
LSDRILDDKAQKVAQTGAATLVVANTPCHLEMLEGIQRAGLADRVQVRHIADVVAEAMRK
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
APAAGGSH
CCCCCCCC
>Mature Secondary Structure
MKVQISEDMILNCMRCGFCLPACPTYRYKQVESASPRGRIALMRAVMEGKLDVLDIAGPL
CEEECCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCCHH
DTCLGCRACEPACPAGVTYGAILEQGRAQLNQVRPLPGVVRWAYRWLLGTPGGIRFSAWA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHH
LWLYQKLRLNALAHRLNLVEKIGGKGLADMERAIPPVASPARRAARKPAYPAAGNRRYRV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHH
AFFMGCLSDIVFFETNQNAIEVLTGLGCEVVLPAGQGCCGAVHSHAGEHELAVAQAKRNI
HHHHHHHHHHEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHCH
AAFETSGCDFIVSTAGGCGAALREYDKLLADDPEWADRARAFSSRCRDFSELVEQLGPIP
HEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
MGDMTGTYTYQDSCHLRNVQKVAAPPRNLLKSMPGARFVELPEADRCCGAAGTYMITQAL
CCCCCCCEEECCCHHHHHHHHHCCCHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHH
LSDRILDDKAQKVAQTGAATLVVANTPCHLEMLEGIQRAGLADRVQVRHIADVVAEAMRK
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
APAAGGSH
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]