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The map label for this gene is truA [H]

Identifier: 149797294

GI number: 149797294

Start: 3256515

End: 3257318

Strand: Reverse

Name: truA [H]

Synonym: STH3042

Alternate gene names: 149797294

Gene position: 3257318-3256515 (Counterclockwise)

Preceding gene: 51894178

Following gene: 51894177

Centisome position: 91.34

GC content: 72.76

Gene sequence:

>804_bases
ATGCGGACCATCAAGCTGATCCTCGCCTACGACGGCACGGCCTATGCCGGCTTTCAGCGGCAGCCCAACGGCATCACCAT
CCAGCAGCGGCTGGAGGAGGCGCTGCGAGCCGTCACCGGCGAGCCCGACCTGCGCATCGGCGCGGCCGCCGGCCGCACGG
ACGCGGGCGTCCACGCCCGGGGCCAGGTGGTGCACCTGCGCACATACGCCACCATTCCCATCGACCGGTGGCCCGCGGCC
CTGAACGGCCATCTCCCGGACGACATCGCCGTCATCGGCGCAGAGGAGGCGCCCCCCGACTTCCACGCCCGGTACTGGGC
CCGGGAGAAGCGGTACCGGTATACGTTCTGCCTTTCCCCGTACCGGCTGCCGATGGCCCGTCTGTACAGCTACCACTGGC
ACCGGCCGGTGGACGTGGACGCGATGCGGCGGGCGGCGGCGCTGCTGGTGGGGCGGCACGACTTCGCCGCCTTCCGGGCG
ACGGGGGGATCGGCCCGCACGACCGTGCGGACGATCACCCGGCTGACGCTCACCGAGGAGCCGCCGTTCCTCCACCTGGA
CGTCACGGCCGATGGCTTCCTCTATAACATGGTGCGGATCATCGCCGGCACGCTGCTGGAGGTGGGCGCAGGCCGGCGCA
GCCTCGACCGGGTGGCCCAGGCCCTGCGGCACGGCCGGCGGGAGGATGCGGGCCGGACCCTGCCGCCCCACGGGCTCTGC
CTGGAAGAGGTGGTCTACGGCGACGGCCCGAGCATTCCGCCCTGGGCCGCGGAGGCCGCGCCGGACGCGGATTTGGAGCC
TTGA

Upstream 100 bases:

>100_bases
GTGACGCCGTCGCCCTTGTGGTCCTGATCGGCTTCGTCGCGCTGATGGTGGTCATGCGGATCGGGGGCGTCGGGTAGCGG
CGCCTGGGGGGAGGTGCGCG

Downstream 100 bases:

>100_bases
CAGTGGTTCGTTCGCCAATGTATACTATGGAGCGTTGACCTCGCCACCTCTGGCGAAAAGAGGTTCTGACCAGGAGCGAC
CTGGACCTCGCGCCTTTTCC

Product: tRNA-pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRTIKLILAYDGTAYAGFQRQPNGITIQQRLEEALRAVTGEPDLRIGAAAGRTDAGVHARGQVVHLRTYATIPIDRWPAA
LNGHLPDDIAVIGAEEAPPDFHARYWAREKRYRYTFCLSPYRLPMARLYSYHWHRPVDVDAMRRAAALLVGRHDFAAFRA
TGGSARTTVRTITRLTLTEEPPFLHLDVTADGFLYNMVRIIAGTLLEVGAGRRSLDRVAQALRHGRREDAGRTLPPHGLC
LEEVVYGDGPSIPPWAAEAAPDADLEP

Sequences:

>Translated_267_residues
MRTIKLILAYDGTAYAGFQRQPNGITIQQRLEEALRAVTGEPDLRIGAAAGRTDAGVHARGQVVHLRTYATIPIDRWPAA
LNGHLPDDIAVIGAEEAPPDFHARYWAREKRYRYTFCLSPYRLPMARLYSYHWHRPVDVDAMRRAAALLVGRHDFAAFRA
TGGSARTTVRTITRLTLTEEPPFLHLDVTADGFLYNMVRIIAGTLLEVGAGRRSLDRVAQALRHGRREDAGRTLPPHGLC
LEEVVYGDGPSIPPWAAEAAPDADLEP
>Mature_267_residues
MRTIKLILAYDGTAYAGFQRQPNGITIQQRLEEALRAVTGEPDLRIGAAAGRTDAGVHARGQVVHLRTYATIPIDRWPAA
LNGHLPDDIAVIGAEEAPPDFHARYWAREKRYRYTFCLSPYRLPMARLYSYHWHRPVDVDAMRRAAALLVGRHDFAAFRA
TGGSARTTVRTITRLTLTEEPPFLHLDVTADGFLYNMVRIIAGTLLEVGAGRRSLDRVAQALRHGRREDAGRTLPPHGLC
LEEVVYGDGPSIPPWAAEAAPDADLEP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=31.5985130111524, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI199559785, Length=246, Percent_Identity=28.8617886178862, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=39.2, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29621; Mature: 29621

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIKLILAYDGTAYAGFQRQPNGITIQQRLEEALRAVTGEPDLRIGAAAGRTDAGVHAR
CCEEEEEEEECCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
GQVVHLRTYATIPIDRWPAALNGHLPDDIAVIGAEEAPPDFHARYWAREKRYRYTFCLSP
CCEEEEEEEEECCHHCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEECC
YRLPMARLYSYHWHRPVDVDAMRRAAALLVGRHDFAAFRATGGSARTTVRTITRLTLTEE
HHCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHEEEEECC
PPFLHLDVTADGFLYNMVRIIAGTLLEVGAGRRSLDRVAQALRHGRREDAGRTLPPHGLC
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
LEEVVYGDGPSIPPWAAEAAPDADLEP
HHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTIKLILAYDGTAYAGFQRQPNGITIQQRLEEALRAVTGEPDLRIGAAAGRTDAGVHAR
CCEEEEEEEECCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
GQVVHLRTYATIPIDRWPAALNGHLPDDIAVIGAEEAPPDFHARYWAREKRYRYTFCLSP
CCEEEEEEEEECCHHCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEECC
YRLPMARLYSYHWHRPVDVDAMRRAAALLVGRHDFAAFRATGGSARTTVRTITRLTLTEE
HHCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHEEEEECC
PPFLHLDVTADGFLYNMVRIIAGTLLEVGAGRRSLDRVAQALRHGRREDAGRTLPPHGLC
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
LEEVVYGDGPSIPPWAAEAAPDADLEP
HHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA