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The map label for this gene is glpF [H]

Identifier: 51598501

GI number: 51598501

Start: 244731

End: 245495

Strand: Direct

Name: glpF [H]

Synonym: BG0243

Alternate gene names: 51598501

Gene position: 244731-245495 (Clockwise)

Preceding gene: 51598498

Following gene: 51598502

Centisome position: 27.06

GC content: 33.86

Gene sequence:

>765_bases
ATGAATTATACGAAATTTCAAGAATTCATATCGGAATTTTTGGGAACATTTATCCTATTAGCTCTAGGAACTGGATCTGT
TGCAATGACAGTATTATTTCCATCAAGTCCTGAAATAACGGGTGAAATAATAAAAGGGGGATATACAAATATAGTATTTG
GATGGGGACTAGGTGTAACGTTTGGTATTTATACATCAGCAAGAATTAGTGGGGCACACCTAAACCCAGCTGTTAGCATA
GGATTGGCAAGTGTTGGAAAATTTCCCGTTTCAAAACTTTTGCATTACATTGTAGCTCAAATATTAGGAGCATTTACAGG
AGCATTAATGACACTTGTTGTATTTTATTCTAAATGGATGGAAATAGATCCTGGTCTAGAAAATACTCAAGGTATAATGG
CAACTTTCCCTGCTGTTCCTGGATTTTTGCCTGGATTTATTGATCAAGTTTTTGGAACCTTTTTGCTAATGTTTTTAATT
TCTGTTGTTGGAGAATTTGTAAAAAAACATGACGATAGTCCATTTATTCCATTTATTATAGGAGCAGTGGTTTTAGCAAT
AGGAATAAGTTTTGGAGGAATGAACGGCTATGCTATTAATCCTGCAAGAGACCTGGGACCAAGAATTTTACTCTTATTTG
CTGGATTTAAAAATCACGGCTTTAATAACCTAAGTATATTTATTGTGCCAATAATTGGTCCAATAATTGGAGCAATTTTA
GGCGCTACAATTTACGAATTTACACTAAAAAACAATAAAGGCTAA

Upstream 100 bases:

>100_bases
CAAAATTTGATTAAAAAATTTAATATATTCAGATTCAAAAATTGAAAATTAAAACTTTTTAAGAAAAAAGAAAATTATTG
TAACAATAAGGAGATAAAAT

Downstream 100 bases:

>100_bases
AAGATATTCAGCTTTATTAAAACTAAAAGGGAAAAATTATGAAATATATTTTATCTATTGATCAAGGTACTACTAGCTCA
AGAGCAATAGTATTTGATAA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFPSSPEITGEIIKGGYTNIVFGWGLGVTFGIYTSARISGAHLNPAVSI
GLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYSKWMEIDPGLENTQGIMATFPAVPGFLPGFIDQVFGTFLLMFLI
SVVGEFVKKHDDSPFIPFIIGAVVLAIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIFIVPIIGPIIGAIL
GATIYEFTLKNNKG

Sequences:

>Translated_254_residues
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFPSSPEITGEIIKGGYTNIVFGWGLGVTFGIYTSARISGAHLNPAVSI
GLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYSKWMEIDPGLENTQGIMATFPAVPGFLPGFIDQVFGTFLLMFLI
SVVGEFVKKHDDSPFIPFIIGAVVLAIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIFIVPIIGPIIGAIL
GATIYEFTLKNNKG
>Mature_254_residues
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFPSSPEITGEIIKGGYTNIVFGWGLGVTFGIYTSARISGAHLNPAVSI
GLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYSKWMEIDPGLENTQGIMATFPAVPGFLPGFIDQVFGTFLLMFLI
SVVGEFVKKHDDSPFIPFIIGAVVLAIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIFIVPIIGPIIGAIL
GATIYEFTLKNNKG

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4502187, Length=251, Percent_Identity=33.4661354581673, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI4826645, Length=257, Percent_Identity=35.408560311284, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI22538420, Length=249, Percent_Identity=34.136546184739, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI157266307, Length=256, Percent_Identity=35.546875, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI310133356, Length=212, Percent_Identity=30.188679245283, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI310114181, Length=212, Percent_Identity=30.188679245283, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4755125, Length=202, Percent_Identity=28.7128712871287, Blast_Score=64, Evalue=9e-11,
Organism=Homo sapiens, GI4502181, Length=202, Percent_Identity=29.2079207920792, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=34.5238095238095, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71992966, Length=255, Percent_Identity=32.5490196078431, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71994009, Length=251, Percent_Identity=36.6533864541833, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17531431, Length=250, Percent_Identity=35.6, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17531429, Length=250, Percent_Identity=35.6, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI32564052, Length=255, Percent_Identity=33.7254901960784, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17533613, Length=255, Percent_Identity=33.7254901960784, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17544068, Length=210, Percent_Identity=38.0952380952381, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71992961, Length=245, Percent_Identity=29.7959183673469, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71993722, Length=257, Percent_Identity=29.1828793774319, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558372, Length=250, Percent_Identity=25.2, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=228, Percent_Identity=35.0877192982456, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322985, Length=291, Percent_Identity=28.1786941580756, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24652747, Length=259, Percent_Identity=25.4826254826255, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551084, Length=259, Percent_Identity=25.4826254826255, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27184; Mature: 27184

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFPSSPEITGEIIKGGYTNIVFGWGLGVT
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCHHH
FGIYTSARISGAHLNPAVSIGLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYSKWM
HHHHHHCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDPGLENTQGIMATFPAVPGFLPGFIDQVFGTFLLMFLISVVGEFVKKHDDSPFIPFII
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GAVVLAIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIFIVPIIGPIIGAIL
HHHHHHHHHHCCCCCCEEECCHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHH
GATIYEFTLKNNKG
HHHHHHEEECCCCC
>Mature Secondary Structure
MNYTKFQEFISEFLGTFILLALGTGSVAMTVLFPSSPEITGEIIKGGYTNIVFGWGLGVT
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCHHH
FGIYTSARISGAHLNPAVSIGLASVGKFPVSKLLHYIVAQILGAFTGALMTLVVFYSKWM
HHHHHHCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDPGLENTQGIMATFPAVPGFLPGFIDQVFGTFLLMFLISVVGEFVKKHDDSPFIPFII
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GAVVLAIGISFGGMNGYAINPARDLGPRILLLFAGFKNHGFNNLSIFIVPIIGPIIGAIL
HHHHHHHHHHCCCCCCEEECCHHHCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHH
GATIYEFTLKNNKG
HHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]