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The map label for this gene is bioH

Identifier: 51598061

GI number: 51598061

Start: 4475770

End: 4476546

Strand: Reverse

Name: bioH

Synonym: YPTB3771

Alternate gene names: 51598061

Gene position: 4476546-4475770 (Counterclockwise)

Preceding gene: 51598064

Following gene: 51598060

Centisome position: 94.35

GC content: 52.25

Gene sequence:

>777_bases
ATGAAGCAGCTTTATTGGTACACCTGCGGTGAAGGCGATTGCGATCTTGTGTTGTTGCACGGATGGGGGCTGAATAGCGG
GGTATGGCATTGCATTATTGATCGACTTGCGCCGCATTTTCGTCTGCATTTGGTGGACCTGCCGGGTTATGGGCGCAGCC
AAGATTATGGTGCGATGTCACTGGCTGACATGGCCGAGAGAGTGGCACAGCAAGCGCCGAAACAGGCATTGTGGCTTGGG
TGGTCAATGGGGGGATTGGTCGCTAGCCAAATAGCCCTGAGCCAGCCAGAGTGTGTCAGGGGGCTGATCACCGTCTCCTC
TTCTCCCTGCTTTACCGCGCGTGATGAATGGCCGGGTATCAAGCCGGAAGTGCTGGCAGGTTTTCAACATCAGCTAAGTG
ATGATTTTCATCGGACAGTAGAACGCTTTTTGGCCTTGCAAACCTTAGGGACTGAAAGTTCGCGTCAGGATGCCCGTTTG
CTAAAATCTGTCGTGCTACAGCATCAAATGCCCGATGTTGAGGTATTGACCGGGGGGTTGGAGATCTTGCGCACGGCGGA
TCTACGTACAGCACTGTCTGGTTTTACGCTGCCTTTTATGCGCGTTTACGGTCATCTGGACAGTTTGGTCCCACGTAAAG
TGGCATCGCTATTAGACAGCGCCTGGCCGCAAACCCAGTCGGTGGTCATGCAAGGGGCTGCTCATGCCCCCTTTATTTCT
CACCCCAATGATTTCGCCAAATTAATCCTGAATTTTGCTGAAGAAAACAAAAAATAA

Upstream 100 bases:

>100_bases
ACGGTTGTTAGCATAGGTATCTTCCTTGATTTTAGTGCCCTTTTAGTGAGCTAAATCACCTGACAGGGCTGTTATTCTTA
TGGCAACAGGATATTACTGT

Downstream 100 bases:

>100_bases
TGGCGTTAGCTTCTTATTTTCTCTCTCTATTTTTACTGCCCAGCCTATATTGGTTGGGTAAAATGGTTGTGTAAAAGAAA
ATTATTTAAGTTATTAAATG

Product: biotin biosynthesis protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKQLYWYTCGEGDCDLVLLHGWGLNSGVWHCIIDRLAPHFRLHLVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLG
WSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGIKPEVLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARL
LKSVVLQHQMPDVEVLTGGLEILRTADLRTALSGFTLPFMRVYGHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFIS
HPNDFAKLILNFAEENKK

Sequences:

>Translated_258_residues
MKQLYWYTCGEGDCDLVLLHGWGLNSGVWHCIIDRLAPHFRLHLVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLG
WSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGIKPEVLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARL
LKSVVLQHQMPDVEVLTGGLEILRTADLRTALSGFTLPFMRVYGHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFIS
HPNDFAKLILNFAEENKK
>Mature_258_residues
MKQLYWYTCGEGDCDLVLLHGWGLNSGVWHCIIDRLAPHFRLHLVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLG
WSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGIKPEVLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARL
LKSVVLQHQMPDVEVLTGGLEILRTADLRTALSGFTLPFMRVYGHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFIS
HPNDFAKLILNFAEENKK

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=255, Percent_Identity=64.7058823529412, Blast_Score=356, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28664; Mature: 28664

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLYWYTCGEGDCDLVLLHGWGLNSGVWHCIIDRLAPHFRLHLVDLPGYGRSQDYGAMS
CCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHH
LADMAERVAQQAPKQALWLGWSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGI
HHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCCCCCCC
KPEVLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARLLKSVVLQHQMPDVEVLTGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
EILRTADLRTALSGFTLPFMRVYGHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFIS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
HPNDFAKLILNFAEENKK
CCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQLYWYTCGEGDCDLVLLHGWGLNSGVWHCIIDRLAPHFRLHLVDLPGYGRSQDYGAMS
CCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHH
LADMAERVAQQAPKQALWLGWSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGI
HHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCCCCCCC
KPEVLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARLLKSVVLQHQMPDVEVLTGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
EILRTADLRTALSGFTLPFMRVYGHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFIS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
HPNDFAKLILNFAEENKK
CCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA