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The map label for this gene is ptsN

Identifier: 51597820

GI number: 51597820

Start: 4195336

End: 4195818

Strand: Direct

Name: ptsN

Synonym: YPTB3528

Alternate gene names: 51597820

Gene position: 4195336-4195818 (Clockwise)

Preceding gene: 51597819

Following gene: 51597821

Centisome position: 88.42

GC content: 46.38

Gene sequence:

>483_bases
ATGACCAACGATCCAGCATTGCAATTAAGCTCGGTACTCAATATCGAGAGCACCAAAAGCTCCGTACACTGCTCAAGTAA
AAAACGGGCTTTGGAAATTATCAGCGAGTTAGCTGCCAAGCAGCTTAATCTACCTTCACAGGTCGTTTTCGATGCGGTAT
TGACCCGTGAACGCATGGGCAGTACCGGGATTGGCAGCGGTATCGCAATACCTCATGGCAAGTTGGAAGAGGATACACTG
CGCGCGGTGGGTGTATTTATTCGTCTGGAACAGCCTATTGCCTTTGATGCGATTGATAACCAACCCGTTGACCTGTTGTT
TGCCTTGTTGGTTCCAGCAGACCAATGTAAAACTCACTTACATACTTTATCTTTAGTCGCCAAACGATTAGCTGATAAAA
CAGTGTGTCGCCGCCTGCGGTCAGCACAAAGTGATGATGAGCTTTATCAGATTATTACTGAATTACCGCCAGAAATAGCC
TAG

Upstream 100 bases:

>100_bases
TTACTCAGCAAAGGTTCTACACTCGCTACATAGCCAGATACCGGTGGATATTTATTCCCACCGGTATCTGGCGTAAAACA
GCGCTTAAGTGAAAGATGAG

Downstream 100 bases:

>100_bases
TTTGGGGCCAATACGCCCAATAGACTTCAATGTGTAGGAAGGCGGTAAAAATAGCGTAAGACAGTAAAAATGTAGGAAGA
CAGGAAAATAGCGTAAGGCA

Product: PTS system transporter subunit IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMGSTGIGSGIAIPHGKLEEDTL
RAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELPPEIA

Sequences:

>Translated_160_residues
MTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMGSTGIGSGIAIPHGKLEEDTL
RAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELPPEIA
>Mature_159_residues
TNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMGSTGIGSGIAIPHGKLEEDTLR
AVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELPPEIA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=152, Percent_Identity=86.1842105263158, Blast_Score=270, Evalue=3e-74,
Organism=Escherichia coli, GI1786951, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17497; Mature: 17366

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMG
CCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
STGIGSGIAIPHGKLEEDTLRAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHL
CCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHCCHHHHHHHH
HTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELPPEIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMG
CCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
STGIGSGIAIPHGKLEEDTLRAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHL
CCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHCCHHHHHHHH
HTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELPPEIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2695747 [H]