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The map label for this gene is outO [H]

Identifier: 51597369

GI number: 51597369

Start: 3606313

End: 3607155

Strand: Reverse

Name: outO [H]

Synonym: YPTB3055

Alternate gene names: 51597369

Gene position: 3607155-3606313 (Counterclockwise)

Preceding gene: 51597370

Following gene: 51597367

Centisome position: 76.03

GC content: 35.71

Gene sequence:

>843_bases
ATGATGGATATACAGGTTTTTTTTGTGAGCTATTTAATTTTTGGGGCCATGGTAGGCAGTTTTCTTAATGTTTTAATCTA
TAGATTGCCTATTATGCTGACTAATTTGTCATCACGATCTGAGTCACACGGTGAAGAAATTAAGATGCGTTCTCATTTGA
GAAACATTAATTTATTTCAACCCGGCTCTTTTTGCCATCATTGCAATGAGTCAATACCAATAAAATATAATATACCGATA
CTCGGTTGGATTTTTCTGCGTGGTGCCAGTCGTTGTTGTAACAAGAAGATAAGCACTCGCTATCTATTTATTGAAGTATT
AGCTGTCATTCAAACACTCTTGGTTCTTATGATTTTTAAAGAGGACTTACTTATCTGCACTAGCTTGGTGCTAATTTGGT
CACTTACGGCATTGGCATTTATAGATTTCGATACCTACCTCCTTCCTGATTGTATGACAATACCTTTGCTGTGGCTTGGT
CTTCTTATCAATATAGATACGGTTTTTGCACCACTAACCTCTGCAGTACTGGGTGCTGTCAGTGGATACCTTTTTCTTTG
GCTATCATATTGGTTGTTTAAAATAGTAAGAGGTGTTGATGGAATGGGCTATGGTGATTTTAAATTAATGGCCGCATTAG
GTGCTTGGTTTGGTGTGAGTGCAGTACCTTTTTTAATATTGTTCTCTTCCTTTTTTGGTCTTGTCGCATATGCTATTTTT
TATTTTTTTGATAAAAAAGATAATGGAAAAGAGATTAATTACATCGCATTTGGCCCTTATATTTCATTGGCGGGTGTTCT
ATACCTGTTTCTTGGGAGTCATGTGACTAATTTATTTTCTTAA

Upstream 100 bases:

>100_bases
TCTAGTGCTGATATATACCTTCACCAAGATGATGATGATGGGTATGTTGCAGGAAAAATTATATTCTTTAAATGAACCAC
TCGGTCTGATAGAGGTAAAC

Downstream 100 bases:

>100_bases
TAAATGGGGTTAATAGCTCCTCTAACTCCAGACACTTAGTATTTTTAGCAGTCAAATCTGATTTGATATCTTGATATAGA
GATTCACTTTTCTTTATGAT

Product: prepilin peptidase

Products: NA

Alternate protein names: Pectic enzymes secretion protein outO; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLTNLSSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPI
LGWIFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPLLWLG
LLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAWFGVSAVPFLILFSSFFGLVAYAIF
YFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS

Sequences:

>Translated_280_residues
MMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLTNLSSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPI
LGWIFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPLLWLG
LLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAWFGVSAVPFLILFSSFFGLVAYAIF
YFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS
>Mature_280_residues
MMDIQVFFVSYLIFGAMVGSFLNVLIYRLPIMLTNLSSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPI
LGWIFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPLLWLG
LLINIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAWFGVSAVPFLILFSSFFGLVAYAIF
YFFDKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=261, Percent_Identity=36.7816091954023, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=45.4545454545455, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31692; Mature: 31692

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8326859; 8065262 [H]