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The map label for this gene is cutF

Identifier: 51597294

GI number: 51597294

Start: 3517624

End: 3518307

Strand: Reverse

Name: cutF

Synonym: YPTB2979

Alternate gene names: 51597294

Gene position: 3518307-3517624 (Counterclockwise)

Preceding gene: 51597295

Following gene: 51597287

Centisome position: 74.15

GC content: 43.27

Gene sequence:

>684_bases
GTGAGTAAATTAACGATAACGCTACTTTTGGCAGTAGGTGCGCTGTCATTATTAGGCTGTAATAATCGCTCTCAACCTGC
TGAACAGGTTTTGCAACCAATGCAGCAGAGCTATCAGGGCGTATTACCTTGTGCGGATTGCAGTGGCTTAGACACTTCTT
TGTTCCTCGATAGTGATGGGACTTTTATCTTGAAAGAAGTTTATCTCGGCACTAAAGATGGCGACCAAACTTTTGCAGAA
TATGGTAAATGGGCACGTACTGCTGATAAGTTAGTGTTAACCAACGGTCAGGGAGAAAAACGTTACTTCCACCCTGTCGA
TAAAAGCTTGGTGATGCTCGATCAGCAAGGTTTACCCATTAAATCGACACTGAATTATCAGTTGGAACCCAGCGACCAGC
CGTTATCCAAAACGCCAATGCCACTAAGTGGTATGTATAAATACTTTGCTGATGCCGCCATATTTACTGATTGTGTAACG
GGTAAGGCGTTCCCGGTAGAGAATAATATCGAACTGGAAACCGGGTATTTAAACGCTCGCAGAAACCCTGGAGAACCTGT
GTTTTTGACCCTCAATGGGTATTTTGATTCAAGGCCATCAATGGAAGAAGGGCGAACGAATAAAACGCTGATTCCGGAGG
GGGATATTCAGTTTAATGCCAATAAAAGTTGTGAGAAAAAATAG

Upstream 100 bases:

>100_bases
AATACATCAAGAGTGAAGTCGATATTCATATGAACGTTGATACATTGCGGTTGATATGACTTCATTCCATATATATCGGA
CACAGTATGGGAGAATCACT

Downstream 100 bases:

>100_bases
CCCTTGTCCATAGCTAAAATAAGAAGCCGCACGGCAGTTCAAGTTCTCCGGCGGCTTCATCAGTAAAATACCCCGCTTAT
GGGGACTCTTTATTTATGGC

Product: lipoprotein involved with copper homeostasis and adhesion

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSKLTITLLLAVGALSLLGCNNRSQPAEQVLQPMQQSYQGVLPCADCSGLDTSLFLDSDGTFILKEVYLGTKDGDQTFAE
YGKWARTADKLVLTNGQGEKRYFHPVDKSLVMLDQQGLPIKSTLNYQLEPSDQPLSKTPMPLSGMYKYFADAAIFTDCVT
GKAFPVENNIELETGYLNARRNPGEPVFLTLNGYFDSRPSMEEGRTNKTLIPEGDIQFNANKSCEKK

Sequences:

>Translated_227_residues
MSKLTITLLLAVGALSLLGCNNRSQPAEQVLQPMQQSYQGVLPCADCSGLDTSLFLDSDGTFILKEVYLGTKDGDQTFAE
YGKWARTADKLVLTNGQGEKRYFHPVDKSLVMLDQQGLPIKSTLNYQLEPSDQPLSKTPMPLSGMYKYFADAAIFTDCVT
GKAFPVENNIELETGYLNARRNPGEPVFLTLNGYFDSRPSMEEGRTNKTLIPEGDIQFNANKSCEKK
>Mature_226_residues
SKLTITLLLAVGALSLLGCNNRSQPAEQVLQPMQQSYQGVLPCADCSGLDTSLFLDSDGTFILKEVYLGTKDGDQTFAEY
GKWARTADKLVLTNGQGEKRYFHPVDKSLVMLDQQGLPIKSTLNYQLEPSDQPLSKTPMPLSGMYKYFADAAIFTDCVTG
KAFPVENNIELETGYLNARRNPGEPVFLTLNGYFDSRPSMEEGRTNKTLIPEGDIQFNANKSCEKK

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=230, Percent_Identity=47.8260869565217, Blast_Score=224, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 25112; Mature: 24981

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLTITLLLAVGALSLLGCNNRSQPAEQVLQPMQQSYQGVLPCADCSGLDTSLFLDSDG
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
TFILKEVYLGTKDGDQTFAEYGKWARTADKLVLTNGQGEKRYFHPVDKSLVMLDQQGLPI
CEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEECCCCCEEEEECCCCCCE
KSTLNYQLEPSDQPLSKTPMPLSGMYKYFADAAIFTDCVTGKAFPVENNIELETGYLNAR
ECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEECC
RNPGEPVFLTLNGYFDSRPSMEEGRTNKTLIPEGDIQFNANKSCEKK
CCCCCEEEEEEECCCCCCCCHHHCCCCCEECCCCCEEECCCCCCCCC
>Mature Secondary Structure 
SKLTITLLLAVGALSLLGCNNRSQPAEQVLQPMQQSYQGVLPCADCSGLDTSLFLDSDG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
TFILKEVYLGTKDGDQTFAEYGKWARTADKLVLTNGQGEKRYFHPVDKSLVMLDQQGLPI
CEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEECCCCCEEEEECCCCCCE
KSTLNYQLEPSDQPLSKTPMPLSGMYKYFADAAIFTDCVTGKAFPVENNIELETGYLNAR
ECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEECC
RNPGEPVFLTLNGYFDSRPSMEEGRTNKTLIPEGDIQFNANKSCEKK
CCCCCEEEEEEECCCCCCCCHHHCCCCCEECCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]