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The map label for this gene is proS

Identifier: 51597293

GI number: 51597293

Start: 3515772

End: 3517490

Strand: Direct

Name: proS

Synonym: YPTB2978

Alternate gene names: 51597293

Gene position: 3515772-3517490 (Clockwise)

Preceding gene: 51597292

Following gene: 51597297

Centisome position: 74.1

GC content: 49.97

Gene sequence:

>1719_bases
ATGCGTACTAGCCAATATCTGCTCTCCACTCAGAAAGAGACACCTGCCGATGCTGAAGTTATCAGCCATCAACTCATGCT
CCGTGCCGGGATGATCCGCAAACTGGCCTCAGGTCTTTACACCTGGCTACCGACCGGGGTTCGGGTGTTGAAAAAAGTTG
AAAACATCGTGCGCGAAGAAATGAATAACGCAGGTGCTATCGAAGTATCAATGCCAGTGGTACAACCCGCGGACTTATGG
CAAGAGAGTGGCCGCTGGGAGCAGTACGGCCCTGAGTTGTTGCGTTTTGTTGACCGCGGCGAGCGCCCTTTTGTTCTTGG
CCCAACCCATGAAGAAGTGATTACTGATCTGATCCGCGGCGAGATTAACTCTTACAAACAACTGCCGCTGAATTTCTTCC
AAATTCAGACCAAGTTTCGTGATGAATTGCGCCCGCGCTTTGGTGTCATGCGCGCCCGTGAGTTTTTGATGAAAGATGCG
TATTCCTTCCATACTACGCAAGAGTCTTTACAGGAAACTTACGACGCGATGTATACCGCGTACAGTAAGATTTTCAGCCG
GATGGATTTGAACTTCCGTGCGGTACTGGCGGATACCGGCTCTATCGGCGGCAGTGCATCCCACGAATTCCAAGTGCTGG
CAGAAAGTGGCGAAGATGATATCGTTTTCTCCACTGGCTCTGATTACGCAGCCAATATTGAATTCGCTGAAGCATTAGCG
CCAACTGAACCACGCGCACCGGCTACAGAAGAGCTGCGAATCGTTGACACCCCGAACGCAAAAACCATCGCCGAGTTGGT
AGAGCAATTCAAACTCCCCATTGAGAAAACCGTGAAAACCTTATTGGTTCATGCTCACGAAGAGAGTGGTCATAAGTTGG
TTGCGCTCCTGGTGCGCGGCGATCATGATTTAAATGAGATCAAAGCAGAGAAGCTGCCACAAGTTGCCAAGCCACTGACC
TTTGCTAGCGAAGAGGAAATTCGTGCAGCTATCGGCGCTGGCCCAGGTTCATTAGGCCCGGTTAATTTATCATTGCCTGT
TATTGCTGACCGCAGTGTGGCGGTGATGAGCGATTTCGGTGCGGGTGCGAATATTGATGGGAAGCATTACTTTGGTATTA
ACTGGGAGCGTGATTTGGCGCTGCCGCTCGTTGCTGATTTGCGTAACGTAGTAGAAGGCGATATCAGCCCTGATGGCAAA
GGCACTCTACAGATCAAACGCGGTATTGAAGTGGGCCATATTTTCCAGTTAGGGACCAAATATTCTGAAGCCATGAAAGC
CACCGTTCAGGGCGAAGATGGCCGTAATCAGGTGATGACCATGGGTTGCTACGGCATAGGGGTATCCCGTGTGGTGGCGG
CGGCTATTGAGCAGAACCATGATGACCGCGGTATCATCTGGCCAGATGCTATTGCGCCATTCCAGGTTGCTATTCTACCT
ATGAATATGCACAAATCTTTCCGAGTGAAAGAACTGGCAGAAGAGCTCTACACCACCCTGCGTTCTCACGGTATTGACGT
TATTCTCGACGACCGTAAAGAGCGCCCAGGTGTGATGTTTGCCGATATGGAATTGATTGGCGTGCCACACAATATCGTCA
TTGGTGACCGTAATCTCGATAGCGAAGAAGTGGAATACAAAAACCGACGCGTCGGTGAAAAACAAATGATTAAGACCAGC
GAAATTGTTGAGTTCTTGCTGAGCCAAATAAAACGCTGA

Upstream 100 bases:

>100_bases
TCACTCTGCTCTCTTTTGACAACCCGTGCTCACTGGTAAACTAGGCCACTTTTTACTTTTTGGCTGCCCCTAATTGCAGT
GCTAATGGAACAAAAACATC

Downstream 100 bases:

>100_bases
ATGGCTATATACCCGTCATAAATAAAGATACCCGCCATAAATAAAGAGTCCCCATAAGCGGGGTATTTTACTGATGAAGC
CGCCGGAGAACTTGAACTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRGEINSYKQLPLNFFQIQTKFRDELRPRFGVMRAREFLMKDA
YSFHTTQESLQETYDAMYTAYSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRGDHDLNEIKAEKLPQVAKPLT
FASEEEIRAAIGAGPGSLGPVNLSLPVIADRSVAVMSDFGAGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGK
GTLQIKRGIEVGHIFQLGTKYSEAMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLDSEEVEYKNRRVGEKQMIKTS
EIVEFLLSQIKR

Sequences:

>Translated_572_residues
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRGEINSYKQLPLNFFQIQTKFRDELRPRFGVMRAREFLMKDA
YSFHTTQESLQETYDAMYTAYSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRGDHDLNEIKAEKLPQVAKPLT
FASEEEIRAAIGAGPGSLGPVNLSLPVIADRSVAVMSDFGAGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGK
GTLQIKRGIEVGHIFQLGTKYSEAMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLDSEEVEYKNRRVGEKQMIKTS
EIVEFLLSQIKR
>Mature_572_residues
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRGEINSYKQLPLNFFQIQTKFRDELRPRFGVMRAREFLMKDA
YSFHTTQESLQETYDAMYTAYSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRGDHDLNEIKAEKLPQVAKPLT
FASEEEIRAAIGAGPGSLGPVNLSLPVIADRSVAVMSDFGAGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGK
GTLQIKRGIEVGHIFQLGTKYSEAMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLDSEEVEYKNRRVGEKQMIKTS
EIVEFLLSQIKR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=219, Percent_Identity=44.7488584474886, Blast_Score=208, Evalue=9e-54,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=84.5884413309983, Blast_Score=1018, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=212, Percent_Identity=41.5094339622642, Blast_Score=175, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=38.2022471910112, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=202, Percent_Identity=41.5841584158416, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=38.1818181818182, Blast_Score=179, Evalue=6e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63862; Mature: 63862

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRG
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EINSYKQLPLNFFQIQTKFRDELRPRFGVMRAREFLMKDAYSFHTTQESLQETYDAMYTA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
HHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCHHHHHCC
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEC
DHDLNEIKAEKLPQVAKPLTFASEEEIRAAIGAGPGSLGPVNLSLPVIADRSVAVMSDFG
CCCHHHHHHHHCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCEEEEECCC
AGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGKGTLQIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
YSEAMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLD
ECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCCEEEEECCCCC
SEEVEYKNRRVGEKQMIKTSEIVEFLLSQIKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLLSTQKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRG
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EINSYKQLPLNFFQIQTKFRDELRPRFGVMRAREFLMKDAYSFHTTQESLQETYDAMYTA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKIFSRMDLNFRAVLADTGSIGGSASHEFQVLAESGEDDIVFSTGSDYAANIEFAEALA
HHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCHHHHHCC
PTEPRAPATEELRIVDTPNAKTIAELVEQFKLPIEKTVKTLLVHAHEESGHKLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEC
DHDLNEIKAEKLPQVAKPLTFASEEEIRAAIGAGPGSLGPVNLSLPVIADRSVAVMSDFG
CCCHHHHHHHHCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCEEEEECCC
AGANIDGKHYFGINWERDLALPLVADLRNVVEGDISPDGKGTLQIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
YSEAMKATVQGEDGRNQVMTMGCYGIGVSRVVAAAIEQNHDDRGIIWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVKELAEELYTTLRSHGIDVILDDRKERPGVMFADMELIGVPHNIVIGDRNLD
ECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCCEEEEECCCCC
SEEVEYKNRRVGEKQMIKTSEIVEFLLSQIKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA