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The map label for this gene is pucI [H]

Identifier: 51597278

GI number: 51597278

Start: 3496321

End: 3497832

Strand: Direct

Name: pucI [H]

Synonym: YPTB2963

Alternate gene names: 51597278

Gene position: 3496321-3497832 (Clockwise)

Preceding gene: 51597276

Following gene: 51597280

Centisome position: 73.69

GC content: 48.68

Gene sequence:

>1512_bases
ATGTTGCCTTTGGAAGGAACGCGCTTTATGCCAAATAATGAAAAAATCTCGGCTAGCAGCATTAACCCCCACTACAGTCC
CAAACTCACTAATGAAGATCTGGCCCCAACTCGTAAGCAGAACTGGGGCTGGTACAATATCTTTTCATTTTGGATGACGG
ATGTGCACAGCATGGGGGGTTATGTGGTCGCCGCCAGCTTCTTTGCTCTCGGGCTATCCAGTTGGCAAGTGCTGTTATCG
CTATTAGTCGGTATTTGCATTGTACAAATTTGCGCCAATCTGGTGGCCAAACCAAGCCAGATAGCCGGTGTGCCCTATGC
CGTTATCTGTCGACAATCTTTTGGGGTATTTGGAGCAAATATCCCAGCCGTTATCCGTGGGCTGATCGCCGTTGCCTGGT
ATGGCATTCAAACCTATCTGGCGGGTAATGCTTTTATGCTCGTGATGCTGAAATTCTTCCCCAGCCTGATCCCCCTGACT
CAGAGCCAATGGCTAGGGTTATCCACCCTCGGCTGGATCTGTTTCTCCATTATGTGGGTACTGCAGGCGATGGTGTTCTG
GCATGGGATGAATGCGATTAAGCGCTTTATTGATTTTGCCGGGCCGGCAGTCTATGTCGTGATGCTGTCATTGGCGTTAT
GGATTGTCTATCAAACCGGCTGGAGTAACATTTCATTCACCCTCGCCAGTAAAATCTTAAGCCCATCAGAACAGGTCTGG
CAGATGCTGACGGCAACCGCGCTGGTCGTCTCTTATTTTGCCGGGCCATTACTGAACTTTGGTGATTTCTCCCGCTATGG
CAAAAGTATGCAGGAAATCCGCCGAGGCAACCGATGGGGGCTACCCTTTAACTTCCTCCTGTTCTCTCTCGTCACCGTGG
TGATTGTCTCCGGCTCACAGTCGCTGTTCGGTGAAATGATTACCGACCCTATCGAGACCGTTACCCGCGTCGGCAATGGC
GTGGCTATCGCTCTAGGTCTACTGACCATGATTATCGCCACTATCGGGATTAATATCGTCGCAAACTTTGTTTCACCCGC
CTTTGATTTTTCTAACTGTTCACCACAAAGAATCAACTTCCGTACTGGTGGGATGATAGCTGCGGTAGGTTCAGTTTTGC
TTACCCCATGGAACCTGTTCCAATCACCAATCATTATCCACTACACCCTGGACGTGCTGGGTGCCTTTATGGGGCCGCTA
TTTGGTATTTTAATCGCTGATTTTTATCTGATTAAACGCGGCCAATTATATGTAGATGATCTGTTTAATGCTTCATCATC
AGGACGTTATTGGTATCGCGGAGGTTTTAATCCGAAAGCGATTATGGCGTTACTGCCCGCAGTGGCCATTGGCTTGATGA
TCATTTTCATCCCCAACCTACAGCAGATCTCCAGTTTCAGTTGGTTTATCGGGGCGTTTCTTGGCGGGAGTATTTATCGC
TTTATTGCACGCAATGACCCATTGCCGAACAATGCGGCTCTGTTTATACGCCCTGAAATAGGCAAAGATTGA

Upstream 100 bases:

>100_bases
ATACAACAATTTTTAGTCTGAGTTCTGCAAATATCTCTAGCCCTAACTCTGTAAATATTTCTCATGTGAGGTTCGACAAA
CCTTGTCTCACATCATCTGA

Downstream 100 bases:

>100_bases
GTTAAGCAGAAACGACAAAGCCCTGAGTTATTACTTAACTCAGGGCTTCTAAATTTTGGCGGTGCGGACGGGACTCGAAC
CCGCGACCCCCGGCGTGACA

Product: nucleobase:cation symporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MLPLEGTRFMPNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFALGLSSWQVLLS
LLVGICIVQICANLVAKPSQIAGVPYAVICRQSFGVFGANIPAVIRGLIAVAWYGIQTYLAGNAFMLVMLKFFPSLIPLT
QSQWLGLSTLGWICFSIMWVLQAMVFWHGMNAIKRFIDFAGPAVYVVMLSLALWIVYQTGWSNISFTLASKILSPSEQVW
QMLTATALVVSYFAGPLLNFGDFSRYGKSMQEIRRGNRWGLPFNFLLFSLVTVVIVSGSQSLFGEMITDPIETVTRVGNG
VAIALGLLTMIIATIGINIVANFVSPAFDFSNCSPQRINFRTGGMIAAVGSVLLTPWNLFQSPIIIHYTLDVLGAFMGPL
FGILIADFYLIKRGQLYVDDLFNASSSGRYWYRGGFNPKAIMALLPAVAIGLMIIFIPNLQQISSFSWFIGAFLGGSIYR
FIARNDPLPNNAALFIRPEIGKD

Sequences:

>Translated_503_residues
MLPLEGTRFMPNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFALGLSSWQVLLS
LLVGICIVQICANLVAKPSQIAGVPYAVICRQSFGVFGANIPAVIRGLIAVAWYGIQTYLAGNAFMLVMLKFFPSLIPLT
QSQWLGLSTLGWICFSIMWVLQAMVFWHGMNAIKRFIDFAGPAVYVVMLSLALWIVYQTGWSNISFTLASKILSPSEQVW
QMLTATALVVSYFAGPLLNFGDFSRYGKSMQEIRRGNRWGLPFNFLLFSLVTVVIVSGSQSLFGEMITDPIETVTRVGNG
VAIALGLLTMIIATIGINIVANFVSPAFDFSNCSPQRINFRTGGMIAAVGSVLLTPWNLFQSPIIIHYTLDVLGAFMGPL
FGILIADFYLIKRGQLYVDDLFNASSSGRYWYRGGFNPKAIMALLPAVAIGLMIIFIPNLQQISSFSWFIGAFLGGSIYR
FIARNDPLPNNAALFIRPEIGKD
>Mature_503_residues
MLPLEGTRFMPNNEKISASSINPHYSPKLTNEDLAPTRKQNWGWYNIFSFWMTDVHSMGGYVVAASFFALGLSSWQVLLS
LLVGICIVQICANLVAKPSQIAGVPYAVICRQSFGVFGANIPAVIRGLIAVAWYGIQTYLAGNAFMLVMLKFFPSLIPLT
QSQWLGLSTLGWICFSIMWVLQAMVFWHGMNAIKRFIDFAGPAVYVVMLSLALWIVYQTGWSNISFTLASKILSPSEQVW
QMLTATALVVSYFAGPLLNFGDFSRYGKSMQEIRRGNRWGLPFNFLLFSLVTVVIVSGSQSLFGEMITDPIETVTRVGNG
VAIALGLLTMIIATIGINIVANFVSPAFDFSNCSPQRINFRTGGMIAAVGSVLLTPWNLFQSPIIIHYTLDVLGAFMGPL
FGILIADFYLIKRGQLYVDDLFNASSSGRYWYRGGFNPKAIMALLPAVAIGLMIIFIPNLQQISSFSWFIGAFLGGSIYR
FIARNDPLPNNAALFIRPEIGKD

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=459, Percent_Identity=28.7581699346405, Blast_Score=207, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6322219, Length=438, Percent_Identity=23.0593607305936, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319495, Length=435, Percent_Identity=22.5287356321839, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319429, Length=438, Percent_Identity=21.0045662100457, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324766, Length=439, Percent_Identity=22.3234624145786, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323266, Length=439, Percent_Identity=23.2346241457859, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324645, Length=446, Percent_Identity=21.7488789237668, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 55557; Mature: 55557

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure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HHHCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]