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The map label for this gene is potH [H]

Identifier: 51597124

GI number: 51597124

Start: 3315644

End: 3316909

Strand: Reverse

Name: potH [H]

Synonym: YPTB2808

Alternate gene names: 51597124

Gene position: 3316909-3315644 (Counterclockwise)

Preceding gene: 51597125

Following gene: 51597123

Centisome position: 69.91

GC content: 52.29

Gene sequence:

>1266_bases
ATGAGCCAAACCGAGATGGTCACCAAACCTGTAGCGGATGAGCGGCAGCAATCTGGCCCGATACTGCGTCAACAACTGCG
CAAAGTGAAGGCGGCCTACACACGCCGATCTCTCTTACTGATTACACCGCTGCTGCTGTTTATCGTCGTTAGCTTTATTT
TCCCCATTGCGACTATCTTGGGGAAAAGTATCGATAACCCCGAATTGAGGGTGGCCATGCCGGTAACAGTGGCTGTACTG
GCGAATTGGCCTGGCGATGCGCTACCGGACGAGGCAATGTTCGAGGCATTGATCACTGATCTGCGTAACGCGCGTAGCAA
TGGATTAATGCTCTCGATCGCCAAACGGATGAGTTATGAAGATAACCGTTATCGTAGCCTGATCATGTCGACACTACGGA
AAGTACCGGCTGAAGGGCCGGTACGTGAAGCGCTAATCAGTGCGTTACCGTTGTGGGGGGAATTAAGCACTTGGCAATCG
CTGCAACGGGCGACTCAGCCCTTGACCCGCTATTACCTGCTATCTGCATTTGACCGTAAAGTAGACGTAGAAAGTGGGCA
AATTAAGGCATTACCGGCTGATCAAGCGTTGTACGTTAACGTCTTGCTGCGAACGTTATGGATGGCGGCTGTCGTCACTC
TGTTATGCGTTACCTTGGGATACCCGCTGGCATATTGGTTGGCAAAACAGCCCACTGGCCGCGCCAACCTGTTGTTGATG
CTGGTGTTGTTACCGTTCTGGACCTCATTGATTGTGCGTACCGCCAGTTGGATTGTGCTGTTGCAGTCCAGTGGGTTAAT
CAACCAGTCGCTGCTGGGGAGTGGGCTTATCGAACAACCATTGGCGCTGGTGTTTAACCGCGCCGGGGTCTACATCTCAA
TGACCCATATCCTGCTGCCCTTTATCGTGTTGCCGCTGTATGCCGTGATGAAAGGTATTTCGCCCAATTATGTCCGAGCC
GCCATTTCATTAGGTGCGCATCCGTTTGCTGCTTTCTGGCGTGTTTATGTACCGCAAACCTACAACGGCGTTACGGCGGG
CGCGTTACTGGTCTTTATGATGGCGATTGGCTACTACATCACACCCGCATTACTCGGCGGTCCGGGTGATCAGATGGTGA
GCTACTTTGTCGCTTTCTTTACCAATACCACCATGAACTGGGGAATGGCCGCGGCGTTGGGGAGCCAGTTATTGATTATC
GTCCTGTTGCTGTACGTGGTTTATATCCGAGTAACCCGTACCCAGGCTGAAACCGCGATTCATTAG

Upstream 100 bases:

>100_bases
GTCGAGCATAGCGAAGATCTTGAGCAACGCTTCAACTCTTGGGCAGCTAAATAACGGCGATAGCTGCTGGCGTAGACCCA
CGGTTTCCGGAGGGAACGCA

Downstream 100 bases:

>100_bases
TTCATCTATTGATGTGTTTATCCGTTCATGAGCTTATCAACACATTGTGCCGGAGGAGACGATGCCGAAACCACAAAAGG
CACTGAAACAAAAAGGGGCG

Product: ABC opine/polyamine transporter, permease subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFIFPIATILGKSIDNPELRVAMPVTVAVL
ANWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLRKVPAEGPVREALISALPLWGELSTWQS
LQRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVTLLCVTLGYPLAYWLAKQPTGRANLLLM
LVLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSGLIEQPLALVFNRAGVYISMTHILLPFIVLPLYAVMKGISPNYVRA
AISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMVSYFVAFFTNTTMNWGMAAALGSQLLII
VLLLYVVYIRVTRTQAETAIH

Sequences:

>Translated_421_residues
MSQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFIFPIATILGKSIDNPELRVAMPVTVAVL
ANWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLRKVPAEGPVREALISALPLWGELSTWQS
LQRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVTLLCVTLGYPLAYWLAKQPTGRANLLLM
LVLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSGLIEQPLALVFNRAGVYISMTHILLPFIVLPLYAVMKGISPNYVRA
AISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMVSYFVAFFTNTTMNWGMAAALGSQLLII
VLLLYVVYIRVTRTQAETAIH
>Mature_420_residues
SQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFIFPIATILGKSIDNPELRVAMPVTVAVLA
NWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYEDNRYRSLIMSTLRKVPAEGPVREALISALPLWGELSTWQSL
QRATQPLTRYYLLSAFDRKVDVESGQIKALPADQALYVNVLLRTLWMAAVVTLLCVTLGYPLAYWLAKQPTGRANLLLML
VLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSGLIEQPLALVFNRAGVYISMTHILLPFIVLPLYAVMKGISPNYVRAA
ISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYITPALLGGPGDQMVSYFVAFFTNTTMNWGMAAALGSQLLIIV
LLLYVVYIRVTRTQAETAIH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=263, Percent_Identity=35.361216730038, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787369, Length=217, Percent_Identity=36.405529953917, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787713, Length=262, Percent_Identity=29.7709923664122, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 46683; Mature: 46552

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFIFPIATIL
CCCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKSIDNPELRVAMPVTVAVLANWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYE
CCCCCCCCEEEECCHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
DNRYRSLIMSTLRKVPAEGPVREALISALPLWGELSTWQSLQRATQPLTRYYLLSAFDRK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VDVESGQIKALPADQALYVNVLLRTLWMAAVVTLLCVTLGYPLAYWLAKQPTGRANLLLM
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LVLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSGLIEQPLALVFNRAGVYISMTHILLP
HHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHH
FIVLPLYAVMKGISPNYVRAAISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYI
HHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TPALLGGPGDQMVSYFVAFFTNTTMNWGMAAALGSQLLIIVLLLYVVYIRVTRTQAETAI
HHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
H
C
>Mature Secondary Structure 
SQTEMVTKPVADERQQSGPILRQQLRKVKAAYTRRSLLLITPLLLFIVVSFIFPIATIL
CCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKSIDNPELRVAMPVTVAVLANWPGDALPDEAMFEALITDLRNARSNGLMLSIAKRMSYE
CCCCCCCCEEEECCHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
DNRYRSLIMSTLRKVPAEGPVREALISALPLWGELSTWQSLQRATQPLTRYYLLSAFDRK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VDVESGQIKALPADQALYVNVLLRTLWMAAVVTLLCVTLGYPLAYWLAKQPTGRANLLLM
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LVLLPFWTSLIVRTASWIVLLQSSGLINQSLLGSGLIEQPLALVFNRAGVYISMTHILLP
HHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHH
FIVLPLYAVMKGISPNYVRAAISLGAHPFAAFWRVYVPQTYNGVTAGALLVFMMAIGYYI
HHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TPALLGGPGDQMVSYFVAFFTNTTMNWGMAAALGSQLLIIVLLLYVVYIRVTRTQAETAI
HHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]