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The map label for this gene is cysK

Identifier: 51597033

GI number: 51597033

Start: 3207162

End: 3208130

Strand: Direct

Name: cysK

Synonym: YPTB2714

Alternate gene names: 51597033

Gene position: 3207162-3208130 (Clockwise)

Preceding gene: 51597032

Following gene: 51597034

Centisome position: 67.6

GC content: 49.95

Gene sequence:

>969_bases
ATGAGCAAGATATATGAAGACAACTCTTTAACAATCGGCCATACGCCGCTGGTTCGTCTGAACCGTATCGGCAACGGACG
TATTTTGGCGAAAGTTGAATCGCGTAACCCAAGTTTCAGTGTGAAGTGCCGTATCGGCGCCAACATGATTTGGGATGCTG
AAAAACGGGGTATTCTGACCGCAGGCAAAGAGCTGGTTGAACCAACCAGCGGGAATACAGGTGTTGCCCTGGCATTTGTC
GCCGCTGCACGTGGCTATAAATTAACCCTTACCATGCCTGAAACCATGAGCATTGAGCGCCGCAAGTTGCTGAAAGCACT
GGGGGCAAATTTGGTGCTTACCGAAGGTGCCAAAGGGATGAAAGGCGCGATTGCCAAAGCGGAAGAAATTCAAGCAACCG
ATCCAGAACGCTACCTTATCTTGCAACAATTCAGTAACCCGGCTAACCCTGAAATTCATGAAAAAACCACCGGCCCTGAA
ATTTGGGAAGATACCGATGGTGATGTAGATGTCTTTGTCTCCGGGGTAGGTACCGGTGGTACCCTAACCGGTGTCAGCCG
CTATATTAAAAACACCAAAGGCAAAGCCATCACGACTGTGGCCGTCGAACCCACTGACTCCCCCGTGATTACCCAAGCGC
TGGCCGGTCAAGAGATCAAACCTGGTCCGCATAAAATTCAAGGTATTGGTGCGGGTTTTATCCCCGGTAACCTCGACTTG
AGTCTGGTTGATCGTGTAGCGCTGGTCACCAATGAAGAAGCTATTTCGATGGCCCGCCGTTTGATGGATGAAGAAGGCAT
TCTGGCGGGTATTTCTTCAGGTGCAGCCGTTGCTGCCGCCGTTAAGCTTTCAGAAGAGGAAGGCTTTACTGACAAAACAA
TTGTGGTTATCCTTCCCTCCTCGGGTGAACGGTATTTAAGTACAGCATTGTTCGCAGATTTGTTCACAGAGCAAGAGCTG
CAACAGTAG

Upstream 100 bases:

>100_bases
TTAATGACATATCGATATACCAATTACTTACTTCCATAGCCATACTGTTCGCGTATGATTTGAACGTTCCCTACATTTTC
ATAGAGTTAAAGGACGGGCT

Downstream 100 bases:

>100_bases
TCTGGGCTACCGCACTAAATGCAAAAAAAGCACCCTTTGGGGTGCTTTTTTTGTGGAGTACATCAAAGTTTTTACTACAT
AAAGATTGATTTTACCCTCC

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILTAGKELVEPTSGNTGVALAFV
AAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPE
IWEDTDGDVDVFVSGVGTGGTLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPSSGERYLSTALFADLFTEQEL
QQ

Sequences:

>Translated_322_residues
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILTAGKELVEPTSGNTGVALAFV
AAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPE
IWEDTDGDVDVFVSGVGTGGTLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPSSGERYLSTALFADLFTEQEL
QQ
>Mature_321_residues
SKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILTAGKELVEPTSGNTGVALAFVA
AARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGMKGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPEI
WEDTDGDVDVFVSGVGTGGTLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDLS
LVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPSSGERYLSTALFADLFTEQELQ
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=327, Percent_Identity=39.4495412844037, Blast_Score=201, Evalue=7e-52,
Organism=Homo sapiens, GI295821200, Length=327, Percent_Identity=39.4495412844037, Blast_Score=201, Evalue=7e-52,
Organism=Homo sapiens, GI4557415, Length=327, Percent_Identity=39.4495412844037, Blast_Score=201, Evalue=7e-52,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=87.9256965944273, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI2367138, Length=311, Percent_Identity=42.1221864951768, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17535051, Length=307, Percent_Identity=53.4201954397394, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17562970, Length=318, Percent_Identity=48.4276729559748, Blast_Score=276, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI115535073, Length=317, Percent_Identity=50.4731861198738, Blast_Score=267, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=49.185667752443, Blast_Score=266, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17561720, Length=316, Percent_Identity=49.6835443037975, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=47.3451327433628, Blast_Score=202, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17534315, Length=318, Percent_Identity=39.3081761006289, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=36.3914373088685, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=47.3282442748092, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=313, Percent_Identity=36.741214057508, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321449, Length=321, Percent_Identity=31.4641744548287, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24643623, Length=314, Percent_Identity=36.9426751592357, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI20129101, Length=314, Percent_Identity=36.9426751592357, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34319; Mature: 34187

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILT
CCCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCEEC
AGKELVEPTSGNTGVALAFVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM
CCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPEIWEDTDGDVDVFVSGVGTGG
HHHHHHHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPS
HHHHEEHEEECHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCCCCEEEEEECC
SGERYLSTALFADLFTEQELQQ
CCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKIYEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCRIGANMIWDAEKRGILT
CCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCEEC
AGKELVEPTSGNTGVALAFVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAKGM
CCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIAKAEEIQATDPERYLILQQFSNPANPEIHEKTTGPEIWEDTDGDVDVFVSGVGTGG
HHHHHHHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TLTGVSRYIKNTKGKAITTVAVEPTDSPVITQALAGQEIKPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
SLVDRVALVTNEEAISMARRLMDEEGILAGISSGAAVAAAVKLSEEEGFTDKTIVVILPS
HHHHEEHEEECHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCCCCEEEEEECC
SGERYLSTALFADLFTEQELQQ
CCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]