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The map label for this gene is truA

Identifier: 51596937

GI number: 51596937

Start: 3093823

End: 3094722

Strand: Reverse

Name: truA

Synonym: YPTB2618

Alternate gene names: 51596937

Gene position: 3094722-3093823 (Counterclockwise)

Preceding gene: 51596938

Following gene: 51596936

Centisome position: 65.23

GC content: 50.56

Gene sequence:

>900_bases
ATGTCCGGGTTTGAATTAGATCAGGAAGAAACATTAGTTCAGCAAAACACATTAGTTCAGCAAAACACATTGGCTCAGCA
AGAAAAACCGGCAGAAGTGGCCGGTTTGAAAATTGCGTTGGGTATTGAGTATAACGGTAGCCGCTATTTTGGTTGGCAAC
GGCAGCAAGAGGTTGCCAGCGTGCAGGCCTGTCTTGAGGCGGCATTATCCAAAGTGGCTAATGAACCCATTGGGGTCTTC
TGCGCTGGCCGTACCGATGCAGGGGTCCATGCTACCGGGCAAGTTGTGCACTTTGTTACCTCTGCAGTACGTAAAGACGC
CGCCTGGACTATGGGGGTCAACAGCCATTTGCCCGCGGATATCGCAGTGCGTTGGGTCAAAACCGTCGATAACGATTTTC
ATGCCCGCTTTAGCGCAACGGCCCGCCGCTATCGCTATATCATTTTTAGCCACCGCTATCGCCCTGCGATATTGGCGCAA
GGGGTCACCCATTGCTATATGCCATTGGATGCAGAAAAAATGGAACGCGCCGCGCAATGTTTACTCGGCGAAAATGATTT
TACCTCTTTCCGTGCCGTACAATGTCAATCCCGCACGCCATGGAGAAATGTAAAACATGTCAAAGTTACCCGACATGGCG
CGTATATCGTGGTAGATATCAAGGCCAACGCGTTTGTACATCATATGGTGCGCAATATCGTTGGGAGCCTGATAGAAATT
GGTTGTGGCAATCAGGACGTGACCTGGATGGCAGAATTGTTGGCGTTGAAGGACAGAACACGTGCTGCCGCAACCGCCAA
AGCCGATGGGTTGTATTTAGTGTCTGTCGATTACCCGGACCACTTCGCGTTACCCAAAGTTCCTATGGGGCCTTTGTTTT
TGGCGGATGACGAGGGTTAA

Upstream 100 bases:

>100_bases
AAGTGCTGCAATTCTGGTCGGTGGCTGACAACATTCGTTTTGGTGGCGCATTGATGGCGATTCAAACGGCAGAGCGCCTG
TTACAGGAGCAACTATACTG

Downstream 100 bases:

>100_bases
CGGTTCACCGGTGACTAAAGATACCGAGAGCCAATTGTCAGGTATGTCGATGGCAAACTGAGGCGTCGCTTTGGGCTAAG
GAATACCTAAGGAAATTCAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSGFELDQEETLVQQNTLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVF
CAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQ
GVTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEI
GCGNQDVTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG

Sequences:

>Translated_299_residues
MSGFELDQEETLVQQNTLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVF
CAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQ
GVTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEI
GCGNQDVTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG
>Mature_298_residues
SGFELDQEETLVQQNTLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFC
AGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQG
VTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIG
CGNQDVTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=283, Percent_Identity=28.9752650176678, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788657, Length=271, Percent_Identity=78.2287822878229, Blast_Score=449, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 33187; Mature: 33056

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFELDQEETLVQQNTLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCEEECCHHHHHHHH
VQACLEAALSKVANEPIGVFCAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPAD
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHH
IAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYMPLDAEKMERAAQC
HHHHHHHHHCCCHHHHHHHHHHHEEEEEEECCCCHHHHHCCCHHEECCCCHHHHHHHHHH
LLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEI
HCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHH
GCGNQDVTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
SGFELDQEETLVQQNTLVQQNTLAQQEKPAEVAGLKIALGIEYNGSRYFGWQRQQEVAS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCEEECCHHHHHHHH
VQACLEAALSKVANEPIGVFCAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNSHLPAD
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHH
IAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYMPLDAEKMERAAQC
HHHHHHHHHCCCHHHHHHHHHHHEEEEEEECCCCHHHHHCCCHHEECCCCHHHHHHHHHH
LLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEI
HCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHH
GCGNQDVTWMAELLALKDRTRAAATAKADGLYLVSVDYPDHFALPKVPMGPLFLADDEG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA