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The map label for this gene is htrB

Identifier: 51596812

GI number: 51596812

Start: 2936394

End: 2937314

Strand: Direct

Name: htrB

Synonym: YPTB2490

Alternate gene names: 51596812

Gene position: 2936394-2937314 (Clockwise)

Preceding gene: 51596808

Following gene: 51596813

Centisome position: 61.89

GC content: 50.38

Gene sequence:

>921_bases
ATGCAAGCTCCTCCTTTTGATCGTTCACTGCTGCACCCACGTTATTGGCTGACCTGGATCGGCCTCGGTATTCTTTACCT
GCTGGTGCTGCTCCCCTACCTGGTTATCTATGCGATGGGAACATCACTGGGTCGTTTTTCTATGCGCTTTCTTAAACGAC
GTAAGCGGATCACACGGCGTAATTTGGAGCTGTGTTTTCCCCATATGTCACCAGAGGAACGTGAGGCGCTGCTGGTCAAG
AACTTTGAATCCGTGGGGATGGGGCTATTTGAAACCGGTATGGCGTGGTTTTGGCCAAATTGGCGTATTGAGCGTTGGAG
CCGTGTCAGTGGTCTGGAACATATTCAACAGGCCCGCGATGAAGGTAAAGGTGTTTTGCTGATCGGGTTACATTTTCTCA
CGCTAGAACTGGGCGCACGTATCTTTGGTATCTACAATCCAGGCATCGGCGTTTATCGCCCCCATGATAATAAAGCAATG
GACTGGATGCAGACATGGGGACGGATGCGTTCGAATAAGGCCATGTTGGACCGCAAAGATCTGAAAGGGATGATCCGTAG
CCTAAAACAAGGTGACATTATCTGGTACGCGCCAGATCATGATTATGGCCCGCGTAGCAGTGTCTTTGTCCCTCTGTTTG
CCGTCGATCAAGCCGCGACCACCACTGGCACTTATTTGCTGGTCCGGATGGGTAAACCCGCCGTTATTCCCTTTACCCCA
CGCCGTTTACCTGACGCCAAGGGATACCAACTGATTTTGCAACCCGCAGTCGAAAACTTCCCGTTAGATAATGAGGTCGA
AGCTGCGGCTTTTATGAACAAAGTCATAGAGAAAGAGATTGCCCTCGCTACTGACCAATATATGTGGCTGCACCGTCGCT
TTAAGACCCGGCCAGAAGGGGAACCTTCTTTATATGAGTAA

Upstream 100 bases:

>100_bases
CAGACTTCTCGTCAAAAAAATATTTTGATCAATAAACCCCTTCATCAATACGGAAACGATTAGCAGTATTAATTAATCAC
AATGACGCTGAGAACTGATA

Downstream 100 bases:

>100_bases
AGATATTAATACCTTACTCCTCGGTGTTGCCACGCTCATGATCCGTCACGTTTTACTGATGTGTCACGTTTAGGGAAATT
GGGTGACAGAATCGGCCCGA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMRFLKRRKRITRRNLELCFPHMSPEEREALLVK
NFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARDEGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAM
DWMQTWGRMRSNKAMLDRKDLKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEGEPSLYE

Sequences:

>Translated_306_residues
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMRFLKRRKRITRRNLELCFPHMSPEEREALLVK
NFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARDEGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAM
DWMQTWGRMRSNKAMLDRKDLKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEGEPSLYE
>Mature_306_residues
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMRFLKRRKRITRRNLELCFPHMSPEEREALLVK
NFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARDEGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAM
DWMQTWGRMRSNKAMLDRKDLKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEGEPSLYE

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=302, Percent_Identity=66.5562913907285, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI87082094, Length=300, Percent_Identity=53.6666666666667, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788161, Length=307, Percent_Identity=28.3387622149837, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35591; Mature: 35591

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMRFLKRRKRITRR
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
NLELCFPHMSPEEREALLVKNFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARD
CHHEEECCCCCHHHHHHHHHCHHHHCCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHH
EGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAMDWMQTWGRMRSNKAMLDRKD
CCCCEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
LKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
HHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEECCCC
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEG
CCCCCCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPSLYE
CCCCCC
>Mature Secondary Structure
MQAPPFDRSLLHPRYWLTWIGLGILYLLVLLPYLVIYAMGTSLGRFSMRFLKRRKRITRR
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
NLELCFPHMSPEEREALLVKNFESVGMGLFETGMAWFWPNWRIERWSRVSGLEHIQQARD
CHHEEECCCCCHHHHHHHHHCHHHHCCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHH
EGKGVLLIGLHFLTLELGARIFGIYNPGIGVYRPHDNKAMDWMQTWGRMRSNKAMLDRKD
CCCCEEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
LKGMIRSLKQGDIIWYAPDHDYGPRSSVFVPLFAVDQAATTTGTYLLVRMGKPAVIPFTP
HHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCEEECCCC
RRLPDAKGYQLILQPAVENFPLDNEVEAAAFMNKVIEKEIALATDQYMWLHRRFKTRPEG
CCCCCCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPSLYE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]