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The map label for this gene is rnt

Identifier: 51596620

GI number: 51596620

Start: 2709311

End: 2709958

Strand: Direct

Name: rnt

Synonym: YPTB2296

Alternate gene names: 51596620

Gene position: 2709311-2709958 (Clockwise)

Preceding gene: 51596619

Following gene: 51596622

Centisome position: 57.1

GC content: 51.39

Gene sequence:

>648_bases
ATGGCAGACAAAAGTGACCTTAACGCCCTGAGTGGCCGTTTTCGTGGGTATTACCCAGTAGTAATTGATGTAGAAACCGC
AGGTTTTAATGCCCAAAGTGATGCATTACTCGAGATTGCCGCAGTAACCTTGCAGATGAATAAAGAGGGCTGGCTGCTAC
CTGATGAAACGTTGCACTTCCATGTGGAACCTTTCGAAGGTGCCAACCTGCAGCCAGAGGCGCTGGCCTTTAATGGGATC
GATCCAACAAATCCTCTACGCGGTGCCGTCAGCGAACATGATGCCTTGCATGCCATTTTTAAAGCCGTGCGTAAGGGAAT
CAAAGATAAAGGCTGTAACCGGGCCATAATCGTGGCACACAATGCCAATTTTGATCACAGCTTTGTTATGGCTGCCGCTG
AACGTGCCAGCTTGAAGCGGAACCCGTTCCACCCGTTTGCCACTTTCGATACCGCCGCTCTCAGTGGGTTGGTATTGGGG
CAAACCGTGCTGGCAAAAGCTTGCCTGGCCGCAGGTATCCCGTTTGACAGTAGTCAGGCGCACTCTGCATTGTACGACAC
CCTGCAAACGGCGAAACTATTCTGTGAGTTGGTTAATCGCTGGAAAAAACTGGGTGGCTGGCCGTTGCCAACGGCAGAAT
CGGAGTAA

Upstream 100 bases:

>100_bases
GCACCTCGGACGGTGCCTTTTACTTATCACAATTAACCCCCTGCATCCCTCGCGAAAATTTGACATAATGCACGCTGAAT
TCGATAAAGATAAGAAACTG

Downstream 100 bases:

>100_bases
CACAGCCCTTGTAATTAAAAAAGGTGGCCATTACGCCACCTGAGGTTATTGACAAAGTGCCCGCGACGGAGAGAACAGGC
AGATCGTAAAGACGCCGTAA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MADKSDLNALSGRFRGYYPVVIDVETAGFNAQSDALLEIAAVTLQMNKEGWLLPDETLHFHVEPFEGANLQPEALAFNGI
DPTNPLRGAVSEHDALHAIFKAVRKGIKDKGCNRAIIVAHNANFDHSFVMAAAERASLKRNPFHPFATFDTAALSGLVLG
QTVLAKACLAAGIPFDSSQAHSALYDTLQTAKLFCELVNRWKKLGGWPLPTAESE

Sequences:

>Translated_215_residues
MADKSDLNALSGRFRGYYPVVIDVETAGFNAQSDALLEIAAVTLQMNKEGWLLPDETLHFHVEPFEGANLQPEALAFNGI
DPTNPLRGAVSEHDALHAIFKAVRKGIKDKGCNRAIIVAHNANFDHSFVMAAAERASLKRNPFHPFATFDTAALSGLVLG
QTVLAKACLAAGIPFDSSQAHSALYDTLQTAKLFCELVNRWKKLGGWPLPTAESE
>Mature_214_residues
ADKSDLNALSGRFRGYYPVVIDVETAGFNAQSDALLEIAAVTLQMNKEGWLLPDETLHFHVEPFEGANLQPEALAFNGID
PTNPLRGAVSEHDALHAIFKAVRKGIKDKGCNRAIIVAHNANFDHSFVMAAAERASLKRNPFHPFATFDTAALSGLVLGQ
TVLAKACLAAGIPFDSSQAHSALYDTLQTAKLFCELVNRWKKLGGWPLPTAESE

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=213, Percent_Identity=78.8732394366197, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 23315; Mature: 23183

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKSDLNALSGRFRGYYPVVIDVETAGFNAQSDALLEIAAVTLQMNKEGWLLPDETLHF
CCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHEEEEEEEECCCCCCCCCCCEEE
HVEPFEGANLQPEALAFNGIDPTNPLRGAVSEHDALHAIFKAVRKGIKDKGCNRAIIVAH
EECCCCCCCCCCHHEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NANFDHSFVMAAAERASLKRNPFHPFATFDTAALSGLVLGQTVLAKACLAAGIPFDSSQA
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTLQTAKLFCELVNRWKKLGGWPLPTAESE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADKSDLNALSGRFRGYYPVVIDVETAGFNAQSDALLEIAAVTLQMNKEGWLLPDETLHF
CCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHEEEEEEEECCCCCCCCCCCEEE
HVEPFEGANLQPEALAFNGIDPTNPLRGAVSEHDALHAIFKAVRKGIKDKGCNRAIIVAH
EECCCCCCCCCCHHEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NANFDHSFVMAAAERASLKRNPFHPFATFDTAALSGLVLGQTVLAKACLAAGIPFDSSQA
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTLQTAKLFCELVNRWKKLGGWPLPTAESE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA