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The map label for this gene is nemA [H]

Identifier: 51596617

GI number: 51596617

Start: 2704253

End: 2705350

Strand: Direct

Name: nemA [H]

Synonym: YPTB2293

Alternate gene names: 51596617

Gene position: 2704253-2705350 (Clockwise)

Preceding gene: 51596616

Following gene: 51596619

Centisome position: 57.0

GC content: 50.91

Gene sequence:

>1098_bases
ATGAAAACTGCTAAATTATTCTCCCCATTAAAGGTTGGCGCGCTCACGTTGCCAAACCGTGTCTTTATGGCCCCACTCAC
CCGCTTGCGTAGCATTGAGCCTGGTGATATCCCAACGCCGTTAATGGCCGAATATTATCGCCAACGGGCCAGTGCAGGCT
TGACTATCACGGAAGCCACTCAGATTTCTTTTCAGGCGAAAGGCTATGCTGGCGCTCCAGGGCTACATACTCAAGAACAG
TTGAACGCATGGAAAAAAGTGACTCAGGGTGTACATAATGAGGGTGGCCATATTGCCGTACAACTTTGGCACGTAGGCCG
TATTTCCCACAACAGTTTACAACCAGGCCAGCAAGCCCCTGTAGCACCATCGGCGATTGCCGCAGATACCCGTACCACCG
TGCGCGATGAAACAGGTGCTTGGGTACGTGTCCCTTGCTCTACCCCACGGGCGCTGGAGACAGAAGAGATTCCGGGCATC
ATTAATGATTTCCGTCAGGCAACCGCCAATGCCCGCGAAGCAGGATTTGACTACATTGAACTGCACGCAGCCCATGGTTA
TCTGTTGCATCAATTCATGTCCCCAGCTTCCAATCAGCGTACTGACCAATATGGCGGCAGTATTGAAAACCGTACCCGTC
TGACGTTGGAAGTGGTCGATGCCACCGCCGCTGAATGGGGGGCTGACCGAATTGGTATTCGTATTTCGCCATTAGGCCCA
TTTAATGGCCTCGATAATGGTGAAGACCAAGAAGAAGCCGCACTGTATCTGATTGATCAGTTGAATAAACGTAACATTGC
TTATTTGCATATTTCCGAGCCAGACTGGGCAGGCGGTAAGCCTTATTCTGATACGTTCCGTGCTGCTGTCCGTGCTCATT
TTAACGGCGTTATTGTTGGTGCTGGTGCTTACAGCGCAGAGAAAGCTGAATGCTTGATTCAAAAAGGGTTCATTGATGCG
GTGGCTTTTGGTCGCAGTTATATTTCTAACCCAGATCTGGTGACGCGCCTGCAACAACACGCGCCACTGAATGAGCCTGA
TAGCAATACTTTCTATGGCGGCGGTGCGAAGGGCTACACCGATTACCCAACGTTGTGA

Upstream 100 bases:

>100_bases
ACAACCAGCCTAATAATCTACCGATCCTGACACTGACTTTTTATGTTTTTCAGGATTTACCCAGCAACTAGTTGACTGGT
CTATTAATACAGGACGAACC

Downstream 100 bases:

>100_bases
TTTGTCTGTAATTTAAATATTCAATAACCGCCAGTTAGGCTAGTCGTCTAACTGGCTCAGACGACTAACAAACCCCGATG
ACGCGATCATGAACGGTGAG

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLTITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVGAGAYSAEKAECLIQKGFIDA
VAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYTDYPTL

Sequences:

>Translated_365_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLTITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVGAGAYSAEKAECLIQKGFIDA
VAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYTDYPTL
>Mature_365_residues
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLTITEATQISFQAKGYAGAPGLHTQEQ
LNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAPVAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGI
INDFRQATANAREAGFDYIELHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVGAGAYSAEKAECLIQKGFIDA
VAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=74.2465753424657, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1789463, Length=242, Percent_Identity=35.1239669421488, Blast_Score=121, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=365, Percent_Identity=28.4931506849315, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=138, Percent_Identity=42.0289855072464, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=143, Percent_Identity=37.0629370629371, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=239, Percent_Identity=30.9623430962343, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=265, Percent_Identity=30.188679245283, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=280, Percent_Identity=28.9285714285714, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=217, Percent_Identity=27.1889400921659, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=38.0577427821522, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321973, Length=384, Percent_Identity=36.1979166666667, Blast_Score=221, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39783; Mature: 39783

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLTITEAT
CCCHHHCCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
QISFQAKGYAGAPGLHTQEQLNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGIINDFRQATANAREAGFDYIE
CCCHHHHCCCCHHEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCHHHCCCCEEE
LHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEE
AGAYSAEKAECLIQKGFIDAVAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYT
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure
MKTAKLFSPLKVGALTLPNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLTITEAT
CCCHHHCCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
QISFQAKGYAGAPGLHTQEQLNAWKKVTQGVHNEGGHIAVQLWHVGRISHNSLQPGQQAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAIAADTRTTVRDETGAWVRVPCSTPRALETEEIPGIINDFRQATANAREAGFDYIE
CCCHHHHCCCCHHEECCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCHHHCCCCEEE
LHAAHGYLLHQFMSPASNQRTDQYGGSIENRTRLTLEVVDATAAEWGADRIGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEEEEEHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEEAALYLIDQLNKRNIAYLHISEPDWAGGKPYSDTFRAAVRAHFNGVIVG
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEE
AGAYSAEKAECLIQKGFIDAVAFGRSYISNPDLVTRLQQHAPLNEPDSNTFYGGGAKGYT
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]