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The map label for this gene is pyrF

Identifier: 51596475

GI number: 51596475

Start: 2528035

End: 2528772

Strand: Direct

Name: pyrF

Synonym: YPTB2149

Alternate gene names: 51596475

Gene position: 2528035-2528772 (Clockwise)

Preceding gene: 51596473

Following gene: 51596476

Centisome position: 53.28

GC content: 52.03

Gene sequence:

>738_bases
ATGACGTCCGCAACTAAAACTAATAACAGTGGCTCAATATCCTCCCCAATTGTTGTTGCGTTGGATTATGCCAATAAAGA
CGCTGCGTTAGCCTTTGCTGATCAGGTTAGTCCACAGGATTGCCGGTTAAAAGTGGGCAAAGAGATGTTTACGCTGTACG
GCCCCGAGCTTATCCGTGACTTACATCAGCGCGGCTTTGACGTATTTCTTGATTTGAAATTCCATGACATCCCTAATACT
ACCGCGCGTGCCGTGGCTGCGGCAGCAGAGTTGGGGGTCTGGATGGTCAATGTTCATGCCAGTGGCGGTGCCCGCATGAT
GTCGGCGGCGAAAGAAGCCTTATTGCCTTATGGTGCACAAGCCCCCTTACTGATTGCCGTTACTGTACTGACCAGCATGG
ATAGCGAAGATCTGCGGGATATCGGTATTACTATCAGTCCGGCTGAGCAGGCCGAGCGGTTAGCGAAACTGACCTGGGAT
TGCGGTTTGGATGGCGTGGTGTGCTCGGCTCATGAAGCGGTGCGTTTGAAGCAAGTTTGTGGCGAAGACTTCTCGCTCGT
TACCCCAGGTATTCGCCCACAAGGCAGTGAAGCCGGTGACCAGCGGCGGATCATGACACCTGAACAGGCGGTAGCTGTAG
GTGTTGATTACATGGTAATCGGTCGCCCAATTACACAATCTCCTGATCCGGAAAAAACGCTGCGCGAAATATTGGCATCA
CTGACGAAGGTGGCATAA

Upstream 100 bases:

>100_bases
CAAATGGAGAATCAACACCCGCCGGATCACTGTAATTTCAGTGATACACCCTGTAGAATATGAGCGTTATCATCAATTAT
TGACTGAAGAAGGCTGAAGA

Downstream 100 bases:

>100_bases
TGAGCAATGATAATAGTCGGTTAGTTTATTCGACTGACAGTGGCCGGATCAGCGAACCAGAGAGCAAAGTTGAGCGGCCA
AAGGGGGATGGCATTGTCCG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTSATKTNNSGSISSPIVVALDYANKDAALAFADQVSPQDCRLKVGKEMFTLYGPELIRDLHQRGFDVFLDLKFHDIPNT
TARAVAAAAELGVWMVNVHASGGARMMSAAKEALLPYGAQAPLLIAVTVLTSMDSEDLRDIGITISPAEQAERLAKLTWD
CGLDGVVCSAHEAVRLKQVCGEDFSLVTPGIRPQGSEAGDQRRIMTPEQAVAVGVDYMVIGRPITQSPDPEKTLREILAS
LTKVA

Sequences:

>Translated_245_residues
MTSATKTNNSGSISSPIVVALDYANKDAALAFADQVSPQDCRLKVGKEMFTLYGPELIRDLHQRGFDVFLDLKFHDIPNT
TARAVAAAAELGVWMVNVHASGGARMMSAAKEALLPYGAQAPLLIAVTVLTSMDSEDLRDIGITISPAEQAERLAKLTWD
CGLDGVVCSAHEAVRLKQVCGEDFSLVTPGIRPQGSEAGDQRRIMTPEQAVAVGVDYMVIGRPITQSPDPEKTLREILAS
LTKVA
>Mature_244_residues
TSATKTNNSGSISSPIVVALDYANKDAALAFADQVSPQDCRLKVGKEMFTLYGPELIRDLHQRGFDVFLDLKFHDIPNTT
ARAVAAAAELGVWMVNVHASGGARMMSAAKEALLPYGAQAPLLIAVTVLTSMDSEDLRDIGITISPAEQAERLAKLTWDC
GLDGVVCSAHEAVRLKQVCGEDFSLVTPGIRPQGSEAGDQRRIMTPEQAVAVGVDYMVIGRPITQSPDPEKTLREILASL
TKVA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=245, Percent_Identity=72.2448979591837, Blast_Score=366, Evalue=1e-103,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 26237; Mature: 26106

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATKTNNSGSISSPIVVALDYANKDAALAFADQVSPQDCRLKVGKEMFTLYGPELIRD
CCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHH
LHQRGFDVFLDLKFHDIPNTTARAVAAAAELGVWMVNVHASGGARMMSAAKEALLPYGAQ
HHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCC
APLLIAVTVLTSMDSEDLRDIGITISPAEQAERLAKLTWDCGLDGVVCSAHEAVRLKQVC
CCHHHHHHHHHCCCHHHHHHCCCEECCHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHC
GEDFSLVTPGIRPQGSEAGDQRRIMTPEQAVAVGVDYMVIGRPITQSPDPEKTLREILAS
CCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHH
LTKVA
HHHCC
>Mature Secondary Structure 
TSATKTNNSGSISSPIVVALDYANKDAALAFADQVSPQDCRLKVGKEMFTLYGPELIRD
CCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHH
LHQRGFDVFLDLKFHDIPNTTARAVAAAAELGVWMVNVHASGGARMMSAAKEALLPYGAQ
HHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCC
APLLIAVTVLTSMDSEDLRDIGITISPAEQAERLAKLTWDCGLDGVVCSAHEAVRLKQVC
CCHHHHHHHHHCCCHHHHHHCCCEECCHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHC
GEDFSLVTPGIRPQGSEAGDQRRIMTPEQAVAVGVDYMVIGRPITQSPDPEKTLREILAS
CCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHH
LTKVA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA