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The map label for this gene is wtpB [H]

Identifier: 51596340

GI number: 51596340

Start: 2371357

End: 2372193

Strand: Reverse

Name: wtpB [H]

Synonym: YPTB2014

Alternate gene names: 51596340

Gene position: 2372193-2371357 (Counterclockwise)

Preceding gene: 51596341

Following gene: 51596339

Centisome position: 50.0

GC content: 52.45

Gene sequence:

>837_bases
ATGAGCCGCACCACCAATGGCCGCCTGGTGGTCACGATTTCGCTGGTTTTTTTCGCCATTGCCAATCTACTGTGGCTGGG
GCTACCGTTCGGTATGGCGATCCTCTGGTCACTGGTTGACCCTCAGTATCCCTGGAGCTATCCAGATATTTTCCCACAGG
TGCTGTCATTTAACCGCTGGCTGCTGGTCTGGGAAAAAACCTCACTTTCCGAAGCACTGATTAACAGTTACACCATCGCG
CCCGTGGTGGCAGCCACCAGCCTACTGCTATCGCTACCTACCGCGTATGCCTTCGGGCGCATGAGCTTTCGCGGCAAGGC
AGTGGCCGAGATGCTAACCCTGATCCCGCTAGTGATGCCGGGAATGATTATCGGCATCTTCTTCAGCGCCATGCTAATGA
ACCTCAATATCAGCAACCCGTTTTTCAGCATCGTGTTGGGCCATACCGTGCTGACGCTACCCTACTCCATCCGCATTATG
TCCGCGGGGTTTAAGGCGGTACCGCAGGATCTGATCGATGCCAGCCGCGATCTGGGTGCTGGCATATGGGGCACCTTCTG
CAACGCCTACCTGCCAATGCTAAAACCGAGTTTTTTGGCAGCGCTGATTTTCTGCTTGGTGAAAAGTCTAGAAGAATTCA
GCATCGCTTATGTCCTTGGCGCACCAGATTTTATTACCGTTCCGACCATTCTTTATTCCTTCCTCGGCTACTCCTTTGTC
CGCCCAGATGCAGCGGTGGTATCAATGATTCTGGTGATCCCCAATGTCATTTTGATGATCATCATTGAGAAGCTTTTGAA
GGGTAATTATCTGTCACAGTCCACCGGGAAAGCCTGA

Upstream 100 bases:

>100_bases
CTGCCGTGATCGCCGTCATTATTATGACCACCGCGGCTATTTTTACGCTGCTGATTGCCACTTTAATCACACCGTTCGCC
ACCCGTAAAGGAGACGTCAA

Downstream 100 bases:

>100_bases
TTTTACAGGAGATAATCACATGAAAAAAACACTGATAACGCTAATGAGCGGGCTGTTATTCAGTTCGCTAACACTCGCAG
CCCAGACCAGCGACTGGGAC

Product: ABC transporter, permease subunit

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSRTTNGRLVVTISLVFFAIANLLWLGLPFGMAILWSLVDPQYPWSYPDIFPQVLSFNRWLLVWEKTSLSEALINSYTIA
PVVAATSLLLSLPTAYAFGRMSFRGKAVAEMLTLIPLVMPGMIIGIFFSAMLMNLNISNPFFSIVLGHTVLTLPYSIRIM
SAGFKAVPQDLIDASRDLGAGIWGTFCNAYLPMLKPSFLAALIFCLVKSLEEFSIAYVLGAPDFITVPTILYSFLGYSFV
RPDAAVVSMILVIPNVILMIIIEKLLKGNYLSQSTGKA

Sequences:

>Translated_278_residues
MSRTTNGRLVVTISLVFFAIANLLWLGLPFGMAILWSLVDPQYPWSYPDIFPQVLSFNRWLLVWEKTSLSEALINSYTIA
PVVAATSLLLSLPTAYAFGRMSFRGKAVAEMLTLIPLVMPGMIIGIFFSAMLMNLNISNPFFSIVLGHTVLTLPYSIRIM
SAGFKAVPQDLIDASRDLGAGIWGTFCNAYLPMLKPSFLAALIFCLVKSLEEFSIAYVLGAPDFITVPTILYSFLGYSFV
RPDAAVVSMILVIPNVILMIIIEKLLKGNYLSQSTGKA
>Mature_277_residues
SRTTNGRLVVTISLVFFAIANLLWLGLPFGMAILWSLVDPQYPWSYPDIFPQVLSFNRWLLVWEKTSLSEALINSYTIAP
VVAATSLLLSLPTAYAFGRMSFRGKAVAEMLTLIPLVMPGMIIGIFFSAMLMNLNISNPFFSIVLGHTVLTLPYSIRIMS
AGFKAVPQDLIDASRDLGAGIWGTFCNAYLPMLKPSFLAALIFCLVKSLEEFSIAYVLGAPDFITVPTILYSFLGYSFVR
PDAAVVSMILVIPNVILMIIIEKLLKGNYLSQSTGKA

Specific function: Part of the ABC transporter complex wtpABC involved in molybdate/tungstate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=221, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787714, Length=208, Percent_Identity=27.8846153846154, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30613; Mature: 30482

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTTNGRLVVTISLVFFAIANLLWLGLPFGMAILWSLVDPQYPWSYPDIFPQVLSFNRW
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCE
LLVWEKTSLSEALINSYTIAPVVAATSLLLSLPTAYAFGRMSFRGKAVAEMLTLIPLVMP
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GMIIGIFFSAMLMNLNISNPFFSIVLGHTVLTLPYSIRIMSAGFKAVPQDLIDASRDLGA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHEEEECCCCHHCCHHHHHHHHHCCC
GIWGTFCNAYLPMLKPSFLAALIFCLVKSLEEFSIAYVLGAPDFITVPTILYSFLGYSFV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCHHHC
RPDAAVVSMILVIPNVILMIIIEKLLKGNYLSQSTGKA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRTTNGRLVVTISLVFFAIANLLWLGLPFGMAILWSLVDPQYPWSYPDIFPQVLSFNRW
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCE
LLVWEKTSLSEALINSYTIAPVVAATSLLLSLPTAYAFGRMSFRGKAVAEMLTLIPLVMP
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GMIIGIFFSAMLMNLNISNPFFSIVLGHTVLTLPYSIRIMSAGFKAVPQDLIDASRDLGA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHEEEECCCCHHCCHHHHHHHHHCCC
GIWGTFCNAYLPMLKPSFLAALIFCLVKSLEEFSIAYVLGAPDFITVPTILYSFLGYSFV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCHHHC
RPDAAVVSMILVIPNVILMIIIEKLLKGNYLSQSTGKA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA