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The map label for this gene is hisF

Identifier: 51595893

GI number: 51595893

Start: 1874593

End: 1875369

Strand: Reverse

Name: hisF

Synonym: YPTB1556

Alternate gene names: 51595893

Gene position: 1875369-1874593 (Counterclockwise)

Preceding gene: 51595894

Following gene: 51595892

Centisome position: 39.53

GC content: 50.97

Gene sequence:

>777_bases
ATGCTGGCAAAACGTATAATTCCTTGTCTGGATGTGAAAGACGGCCAGGTCGTTAAAGGTGTTCAGTTCCGCAATCATGA
GATCATCGGCGATATCGTGCCTTTGGCGCAGCGCTATGCTCAAGAAGGGGCTGATGAGTTGGTGTTTTACGATATCACTG
CCTCATCCGATGGCCGTGTCGTCGATAAAAGCTGGGTAGCGCGGGTCGCAGAAGTGATCGATATCCCTTTCTGTGTGGCC
GGGGGGATCAAAAGTGTCGAAGATGCAAGCCAGATCCTGACATTCGGTGCGGATAAAATCTCAATCAACTCTCCCGCATT
GGCAGACCCAACACTCATTACCCGCTTGGCTGACCGCTATGGCGTACAATGCATCGTGGTGGGAATTGATACCTGGTATG
ACACCGAAAGCGACAGCTATCAGGTTTACCAGTTTACCGGTGATGAGAAGCGTACCAAAGCCACCACGTGGCAAACCGAA
GATTGGGTCAAAGAGGTCCAGTTACGGGGAGCCGGTGAGATTGTATTAAATATGATGAATCAGGACGGCGTCCGTAACGG
CTATGATTTGCGTCAGCTTCAACAGATGCGTGCTATTTGCCACGTTCCGTTAATCGCCTCTGGCGGTGCCGGGACACCGG
AGCACTTCCTTGAAGCCTTCCGCGACGCGGATGTTGATGGCGCGCTGGCGGCATCGGTATTCCATAAACAAATCATTAAC
ATCGGCGAATTGAAAAAGTATTTGTCTGAACAGGGCGTGGAGATAAGAGTGTGTTAA

Upstream 100 bases:

>100_bases
GTCTGGATGATATCGCCCGCTTACGCGGCAGCGGTGTACAGGGCGTGATTGTTGGCCGTGCGTTACTGGACGGCAAGTTT
AACGTGAAGGAGGCTATCGC

Downstream 100 bases:

>100_bases
CAGAACAACAAATCAACCAATTGGATTGGGAAAAAGTCGATCACCTGATGCCAGCCATCGTTCAGCATGCCGTATCCGGC
GAAGTTCTGATGATGGGGTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSVEDASQILTFGADKISINSPALADPTLITRLADRYGVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTE
DWVKEVQLRGAGEIVLNMMNQDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPEHFLEAFRDADVDGALAASVFHKQIIN
IGELKKYLSEQGVEIRVC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSVEDASQILTFGADKISINSPALADPTLITRLADRYGVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTE
DWVKEVQLRGAGEIVLNMMNQDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPEHFLEAFRDADVDGALAASVFHKQIIN
IGELKKYLSEQGVEIRVC
>Mature_258_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKSVEDASQILTFGADKISINSPALADPTLITRLADRYGVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTE
DWVKEVQLRGAGEIVLNMMNQDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPEHFLEAFRDADVDGALAASVFHKQIIN
IGELKKYLSEQGVEIRVC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=86.4341085271318, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI87082028, Length=234, Percent_Identity=24.3589743589744, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=31.2302839116719, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28525; Mature: 28525

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSVEDASQILTFGADKISINSPALADPTLITRLADRY
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTEDWVKEVQLRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
IGELKKYLSEQGVEIRVC
HHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKSVEDASQILTFGADKISINSPALADPTLITRLADRY
ECHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDTESDSYQVYQFTGDEKRTKATTWQTEDWVKEVQLRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLRQLQQMRAICHVPLIASGGAGTPEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
IGELKKYLSEQGVEIRVC
HHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA