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The map label for this gene is rumB

Identifier: 51595706

GI number: 51595706

Start: 1627424

End: 1628554

Strand: Direct

Name: rumB

Synonym: YPTB1366

Alternate gene names: 51595706

Gene position: 1627424-1628554 (Clockwise)

Preceding gene: 51595705

Following gene: 51595708

Centisome position: 34.3

GC content: 52.34

Gene sequence:

>1131_bases
ATGCATTGCGCACAGTATACGGCAGGTCGCTGCCGTTCTTGTCAGTGGTTGGATAAACCCTATCCACAACAACTCGCTGA
TAAACAGCATCATCTGGAAAGCCTGTTGGCTGGGCATGCGGTCACCCAGTGGCTAGCACCGGTTTTTGGGCGCGAAAGTA
CCTTTCGTAACAAGGCGAAAATGGTCGTCAGCGGCAGTGTGGAACGCCCGTTACTGGGTATGCTGCATCGTGACGGTACA
CCGGTTGATTTATGCGCATGTCCACTTTATCCGCCCAGCTTCGAACCGGTATTTACGGTACTGAAAACCTTTATTGCCAG
GGCGGGTTTGACTCCCTATAACGTTGCTCGCAAGCGTGGCGAACTTAAATTCCTGTTACTCACGGAAAGTACCTACAACG
GTGAATTGATGCTGCGCTTTGTGCTGCGTTCTGAAACTAAATTAGCGCAGTTAATTGCTGCGTTGCCGTGGCTGCAACAA
CAGTTGCCGCAGTTGGCGGTGATCTCGGCTAATATTCAGCCAGTGCATATGGCCATTCTGGAAGGGGAGCGGGAGATCCC
GCTGACCGAACAACAGGCCCTGCCTGAGCGGTTTAATCAGGTGCCGTTGTATATCCGCCCACAAAGTTTTTTCCAGACCA
ATCCACCGGTGGCGGCTTCGTTGTATGCAACAGCACGGCAGTGGGTGCAGGAGCATGAGGTTCACAGTATGTGGGATCTG
TTCTGTGGTGTGGGCGGCTTTGGTTTACATTGTGCGGGGCCAGAGACTCAATTGACCGGCATTGAAATCAGTGCTGAAGC
TATCGCCTGTGCCCGCCAGTCGGCTGAGCAGTTAGGGCTAAAAAATGTCAGTTTCGCCGCGCTGGATTCTACCCGCTTTG
CTACCGCTGAAGCCCAAATACCTGAACTGGTCTTGGTGAATCCACCACGGCGGGGGATCGGCCGCGAGTTATGTGATTAC
CTGAGCCAGATGGCACCTAAATTTATTCTCTATTCAAGTTGTAATGCAGAGACGATGGCGAAAGATATCAGTTTGCTTGC
GGGTTACCACATTGAACGGGTACAGCTGTTTGATATGTTCCCGCATACCAGTCACTACGAAGTGCTCACCTTGCTGGCTC
TCCGTCGCTAG

Upstream 100 bases:

>100_bases
CAAAAATGAGTGTTTAACGCCATAGGGTGCTACAATCCGCGCCCATCGACAGCTTCGGCTGCACATTATCTACTGTCTCA
GAGTATAACGGTCAATATTA

Downstream 100 bases:

>100_bases
ACGTGGTTTGTGGCTTAAGTGAGTATGCAGTGAGCCAAAGTCAAAGCGTTAAAACGTTAAAGTCACTGGCGTTGCAGGTC
GGCAGCTAACAAGCGCATCC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESTFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLALRR

Sequences:

>Translated_376_residues
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESTFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLALRR
>Mature_376_residues
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESTFRNKAKMVVSGSVERPLLGMLHRDGT
PVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRGELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQ
QLPQLAVISANIQPVHMAILEGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQIPELVLVNPPRRGIGRELCDY
LSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMFPHTSHYEVLTLLALRR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=226, Percent_Identity=27.8761061946903, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI51173878, Length=226, Percent_Identity=27.8761061946903, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1787083, Length=372, Percent_Identity=74.7311827956989, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789148, Length=375, Percent_Identity=26.4, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790403, Length=156, Percent_Identity=30.1282051282051, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666579, Length=414, Percent_Identity=24.6376811594203, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 42144; Mature: 42144

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESTFRNKAK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHE
MVVSGSVERPLLGMLHRDGTPVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQQLPQLAVISANIQPVHMAIL
CEEEEEEECCCCCCHHEEHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
EGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQI
HHCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
PELVLVNPPRRGIGRELCDYLSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMF
CEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHHEEEECCC
PHTSHYEVLTLLALRR
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHCAQYTAGRCRSCQWLDKPYPQQLADKQHHLESLLAGHAVTQWLAPVFGRESTFRNKAK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHE
MVVSGSVERPLLGMLHRDGTPVDLCACPLYPPSFEPVFTVLKTFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKFLLLTESTYNGELMLRFVLRSETKLAQLIAALPWLQQQLPQLAVISANIQPVHMAIL
CEEEEEEECCCCCCHHEEHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
EGEREIPLTEQQALPERFNQVPLYIRPQSFFQTNPPVAASLYATARQWVQEHEVHSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCAGPETQLTGIEISAEAIACARQSAEQLGLKNVSFAALDSTRFATAEAQI
HHCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCC
PELVLVNPPRRGIGRELCDYLSQMAPKFILYSSCNAETMAKDISLLAGYHIERVQLFDMF
CEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHHEEEECCC
PHTSHYEVLTLLALRR
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA