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The map label for this gene is amtB

Identifier: 51595324

GI number: 51595324

Start: 1171625

End: 1172920

Strand: Direct

Name: amtB

Synonym: YPTB0974

Alternate gene names: 51595324

Gene position: 1171625-1172920 (Clockwise)

Preceding gene: 51595323

Following gene: 51595326

Centisome position: 24.69

GC content: 51.39

Gene sequence:

>1296_bases
TTGATGATGAAAAAACTTTTATCCGTGTTGGGCCTGGGTACGGTAGCGCTGTTACCTTCTTGGGCAATGGCGGCGGCACC
GGCAGTCGCTGACAAAGCAGATAATGCTTTTATGATGATTTGCACTGCGTTAGTACTGTTTATGACGATACCCGGTATTG
CCCTGTTCTACGGTGGCCTGTTGCGCTCCAAAAACGTATTGTCAATGATGACTCAGGTCATGGTGAGTTTTGCGCTGGTA
TGTGTTTTGTGGATTCTCTACGGCTACAGCCTGGCGTTTAGTGCGGGTAATGCGTTCTTCGGCAGTTTTGATATGGCCAT
GCTGAAAGGTATTGAGCTGACTAGCCTGAGTGGCTCGTTTTATCAATATATTCATGTCGTGTTCCAAGCCTCATTTGCCT
GTATCACCGTCGCTCTGATTGTGGGTTCATTCGCAGAGCGTATTCGTTTCTCAGCAGTATTAATTTTCGCCGCGCTGTGG
TTCACCTTCTCTTACCTACCAATAGCGCACATGGTATGGGGTGGTGGTTTCCTGGCAATGGACGGAGCGCTGGATTTTGC
TGGAGGTACAGTGGTGCACATTAACGCCGCTGTTGCTGGTTTGGTCGGGGCTTATCTGTTGGGTAAACGTGCCGGATTCG
GTAAAGAGGCCTTCAAACCCCATAACTTACCGATGGTCTTTACCGGTACGGCGATTCTGTATATTGGCTGGTTTGGCTTC
AATGCCGGTTCAGCCAGTGCTGCGAACGAAATTGCAGCGCTAGCGTTCTTGAATACCGTCGTTGCAACGGCGGGCGCCAT
CCTCTCGTGGGTATTTGCTGAGTGGGCTTTACGAGGTAAACCTTCGCTGTTGGGGGCATGTTCCGGTTGTATCGCAGGCT
TGGTTGCGATTACTCCGGCAGCAGGTACGGTAGGGGTTGGCGGTGCATTGATTATCGGTTTGGTTGGTGGTGTTGCTGGC
CTGTGGGGGGTCGTGCTACTGAAAAAATGGCTGCGGGTCGATGATACCTGTGATGTCTTCGGTGTTCATGGTGTGTGTGG
CATCGTCGGTTGTATTCTGACGGGTGTCTTCACCTCGGCGTCGCTGGGCGGCACTGGTTATGCTGCTGGCGTAACCATGG
GGCATCAGGTCGGCGTTCAGTTATTCAGTGTTGGTGTGACTGTTGTCTGGTCGGGTGTTGTTGCCTTCGTTGCCTTTAAA
ATTGCGGGCTTGATTGTGGGCCTACGGGTACCCGAAGAGCAAGAGCGTGAAGGTCTGGATGTTAACAGTCACGGTGAAAA
CGCATATAATCAATAA

Upstream 100 bases:

>100_bases
TTTCGTTGCTGAATTGCAACGTGTTATTCGCATCCGTACTGGCGAAACAGACGAAGCAGCGCTGTAATTTGCAGGCTCAA
ATTTAGAGAATAGGGATGGG

Downstream 100 bases:

>100_bases
GTTAATTTTCCGTCAATCGTTAGTTGTTAGCCTGTCGGAAGAGATAGAGCAGTAAGTTAAAAAGAAAAAGACATAAAGAA
AAGAGGGGCAGTTGTTGGAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MMMKKLLSVLGLGTVALLPSWAMAAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGLLRSKNVLSMMTQVMVSFALV
CVLWILYGYSLAFSAGNAFFGSFDMAMLKGIELTSLSGSFYQYIHVVFQASFACITVALIVGSFAERIRFSAVLIFAALW
FTFSYLPIAHMVWGGGFLAMDGALDFAGGTVVHINAAVAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGTAILYIGWFGF
NAGSASAANEIAALAFLNTVVATAGAILSWVFAEWALRGKPSLLGACSGCIAGLVAITPAAGTVGVGGALIIGLVGGVAG
LWGVVLLKKWLRVDDTCDVFGVHGVCGIVGCILTGVFTSASLGGTGYAAGVTMGHQVGVQLFSVGVTVVWSGVVAFVAFK
IAGLIVGLRVPEEQEREGLDVNSHGENAYNQ

Sequences:

>Translated_431_residues
MMMKKLLSVLGLGTVALLPSWAMAAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGLLRSKNVLSMMTQVMVSFALV
CVLWILYGYSLAFSAGNAFFGSFDMAMLKGIELTSLSGSFYQYIHVVFQASFACITVALIVGSFAERIRFSAVLIFAALW
FTFSYLPIAHMVWGGGFLAMDGALDFAGGTVVHINAAVAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGTAILYIGWFGF
NAGSASAANEIAALAFLNTVVATAGAILSWVFAEWALRGKPSLLGACSGCIAGLVAITPAAGTVGVGGALIIGLVGGVAG
LWGVVLLKKWLRVDDTCDVFGVHGVCGIVGCILTGVFTSASLGGTGYAAGVTMGHQVGVQLFSVGVTVVWSGVVAFVAFK
IAGLIVGLRVPEEQEREGLDVNSHGENAYNQ
>Mature_431_residues
MMMKKLLSVLGLGTVALLPSWAMAAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGLLRSKNVLSMMTQVMVSFALV
CVLWILYGYSLAFSAGNAFFGSFDMAMLKGIELTSLSGSFYQYIHVVFQASFACITVALIVGSFAERIRFSAVLIFAALW
FTFSYLPIAHMVWGGGFLAMDGALDFAGGTVVHINAAVAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGTAILYIGWFGF
NAGSASAANEIAALAFLNTVVATAGAILSWVFAEWALRGKPSLLGACSGCIAGLVAITPAAGTVGVGGALIIGLVGGVAG
LWGVVLLKKWLRVDDTCDVFGVHGVCGIVGCILTGVFTSASLGGTGYAAGVTMGHQVGVQLFSVGVTVVWSGVVAFVAFK
IAGLIVGLRVPEEQEREGLDVNSHGENAYNQ

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=420, Percent_Identity=79.0476190476191, Blast_Score=676, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550182, Length=435, Percent_Identity=29.4252873563218, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=429, Percent_Identity=27.7389277389277, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=430, Percent_Identity=25.8139534883721, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32564042, Length=414, Percent_Identity=25.3623188405797, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325396, Length=415, Percent_Identity=35.6626506024096, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=410, Percent_Identity=33.9024390243902, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6324187, Length=422, Percent_Identity=36.4928909952607, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI161078314, Length=432, Percent_Identity=26.8518518518519, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44786; Mature: 44786

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMKKLLSVLGLGTVALLPSWAMAAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGL
CCHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRSKNVLSMMTQVMVSFALVCVLWILYGYSLAFSAGNAFFGSFDMAMLKGIELTSLSGSF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCEEEECCHHH
YQYIHVVFQASFACITVALIVGSFAERIRFSAVLIFAALWFTFSYLPIAHMVWGGGFLAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
DGALDFAGGTVVHINAAVAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGTAILYIGWFGF
CCCEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEEEECC
NAGSASAANEIAALAFLNTVVATAGAILSWVFAEWALRGKPSLLGACSGCIAGLVAITPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
AGTVGVGGALIIGLVGGVAGLWGVVLLKKWLRVDDTCDVFGVHGVCGIVGCILTGVFTSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
SLGGTGYAAGVTMGHQVGVQLFSVGVTVVWSGVVAFVAFKIAGLIVGLRVPEEQEREGLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
VNSHGENAYNQ
CCCCCCCCCCC
>Mature Secondary Structure
MMMKKLLSVLGLGTVALLPSWAMAAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGL
CCHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRSKNVLSMMTQVMVSFALVCVLWILYGYSLAFSAGNAFFGSFDMAMLKGIELTSLSGSF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCEEEECCHHH
YQYIHVVFQASFACITVALIVGSFAERIRFSAVLIFAALWFTFSYLPIAHMVWGGGFLAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
DGALDFAGGTVVHINAAVAGLVGAYLLGKRAGFGKEAFKPHNLPMVFTGTAILYIGWFGF
CCCEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEEEEEEECC
NAGSASAANEIAALAFLNTVVATAGAILSWVFAEWALRGKPSLLGACSGCIAGLVAITPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
AGTVGVGGALIIGLVGGVAGLWGVVLLKKWLRVDDTCDVFGVHGVCGIVGCILTGVFTSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
SLGGTGYAAGVTMGHQVGVQLFSVGVTVVWSGVVAFVAFKIAGLIVGLRVPEEQEREGLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
VNSHGENAYNQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]