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The map label for this gene is sbcD

Identifier: 51595266

GI number: 51595266

Start: 1105348

End: 1106592

Strand: Reverse

Name: sbcD

Synonym: YPTB0916

Alternate gene names: 51595266

Gene position: 1106592-1105348 (Counterclockwise)

Preceding gene: 51595274

Following gene: 51595265

Centisome position: 23.32

GC content: 48.76

Gene sequence:

>1245_bases
ATGCGTATTATTCATACCTCTGACTGGCATTTGGGGCAGTATTTTTTCACCAAAAGCCGTGCTGCTGAACATCAGGCATT
TCTGCACTGGTTGATTGAGCAGATCAAAGAACACCAGGTTGATGCGCTGATTGTCGCAGGGGATATTTTTGATACCGGTG
CCCCGCCCAGCTATGCCCGTGAGCTCTATAACCGTTTTATTGTTGAGCTCCAGCCTACCGCCTGTCAATTAGTGGTACTG
GGTGGCAACCATGATTCGGTAGCCACACTGAATGAATCACGAGATTTACTTTCATACCTGAATACCTCGGTAATCTCATG
TGCCAGTCGTGATCTGAAACAACAAGTGATTATCCTGAAAAATAGACAGCAGCAACCCGCAGCGGTGCTATGCGCCATTC
CGTTTTTACGGCCCCGTGATCTGGTGACCAGTCAAGCGGGTGAGTCTGGTGACCAGAAACAACTGGCATTACAAGAAGCT
ATTGCTGGCCACTATCAAACGTTATATCAACGCGCCCTAGAACTGCGATCTCAGCTTGGCTTGCCTCTGCCCATTATCGC
CACCGGGCACCTGACCACCGTGGGTGTGACAACCACAGACTCAGTACGAGACATTTATATTGGTACACTAGATGCCTTTC
CGGCTCAGGCTTTTCCACCCGCGGATTACATTGCCCTTGGTCATATCCATCGCGCTCAACAGGTCGCTAAAACTGAACAT
ATCCGCTACAGCGGCTCACCTATAGCGCTCAGTTTTGATGAACTAAGCAAAGAGAAAAGTGTGTATTTGGTGGAGTTTGA
CCAGCAAAATCTGTCATCAGTTACCCCACTCTTCATTCCACAATTTCAACCGATGCAATTGATCAAAGGTGATTTGCGCC
AAATAGAGCAGCAATTAGCGGCATTTGCTGGCCATGAAGGCTTACCCGTGTGGTTGGATATAGAAGTGGCAACCCAAGAC
TATCTCACCGATATTCAAAGACGGATCCAGGCACTGGCAGCGAACTTGCCCGTTGAAGTGGTATTACTACGCCGTAGCAA
AGAACAACGTAATAACAGCATCCACCGGCAGGAAAAAGAAACGCTAAACGAATTGACCGTCAGTGACGTATTTGAACGTA
GGCTGGCACTGGAGGCAGATTTATCCGAGCCTCGCCAGCAGCGAATGCGCCAAATGTTCAATTACGTGGTTGATGAAATA
GCACAAGATGGCAGTAACGGTGTGGCGGAGGAGCCAGCCCAATGA

Upstream 100 bases:

>100_bases
AGATTTATTTCATCCTGTGTTTTTCACCGCGACATCTTGATGCCAACGCTTATAATCAGCGCCATTCTCTTTCGTCATCA
TCGGCCATAGCGAGAGCTTT

Downstream 100 bases:

>100_bases
AAATTTTGAGCCTACGTCTGAAAAACATTAACTCATTACAGGGCGAATGGAAGATAGATTTCACTGCCGAACCTTTTGCC
AGTAACGGCTTATTTGCTAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRIIHTSDWHLGQYFFTKSRAAEHQAFLHWLIEQIKEHQVDALIVAGDIFDTGAPPSYARELYNRFIVELQPTACQLVVL
GGNHDSVATLNESRDLLSYLNTSVISCASRDLKQQVIILKNRQQQPAAVLCAIPFLRPRDLVTSQAGESGDQKQLALQEA
IAGHYQTLYQRALELRSQLGLPLPIIATGHLTTVGVTTTDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQQVAKTEH
IRYSGSPIALSFDELSKEKSVYLVEFDQQNLSSVTPLFIPQFQPMQLIKGDLRQIEQQLAAFAGHEGLPVWLDIEVATQD
YLTDIQRRIQALAANLPVEVVLLRRSKEQRNNSIHRQEKETLNELTVSDVFERRLALEADLSEPRQQRMRQMFNYVVDEI
AQDGSNGVAEEPAQ

Sequences:

>Translated_414_residues
MRIIHTSDWHLGQYFFTKSRAAEHQAFLHWLIEQIKEHQVDALIVAGDIFDTGAPPSYARELYNRFIVELQPTACQLVVL
GGNHDSVATLNESRDLLSYLNTSVISCASRDLKQQVIILKNRQQQPAAVLCAIPFLRPRDLVTSQAGESGDQKQLALQEA
IAGHYQTLYQRALELRSQLGLPLPIIATGHLTTVGVTTTDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQQVAKTEH
IRYSGSPIALSFDELSKEKSVYLVEFDQQNLSSVTPLFIPQFQPMQLIKGDLRQIEQQLAAFAGHEGLPVWLDIEVATQD
YLTDIQRRIQALAANLPVEVVLLRRSKEQRNNSIHRQEKETLNELTVSDVFERRLALEADLSEPRQQRMRQMFNYVVDEI
AQDGSNGVAEEPAQ
>Mature_414_residues
MRIIHTSDWHLGQYFFTKSRAAEHQAFLHWLIEQIKEHQVDALIVAGDIFDTGAPPSYARELYNRFIVELQPTACQLVVL
GGNHDSVATLNESRDLLSYLNTSVISCASRDLKQQVIILKNRQQQPAAVLCAIPFLRPRDLVTSQAGESGDQKQLALQEA
IAGHYQTLYQRALELRSQLGLPLPIIATGHLTTVGVTTTDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQQVAKTEH
IRYSGSPIALSFDELSKEKSVYLVEFDQQNLSSVTPLFIPQFQPMQLIKGDLRQIEQQLAAFAGHEGLPVWLDIEVATQD
YLTDIQRRIQALAANLPVEVVLLRRSKEQRNNSIHRQEKETLNELTVSDVFERRLALEADLSEPRQQRMRQMFNYVVDEI
AQDGSNGVAEEPAQ

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=402, Percent_Identity=60.6965174129353, Blast_Score=487, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: 3.1.-.-

Molecular weight: Translated: 46608; Mature: 46608

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIHTSDWHLGQYFFTKSRAAEHQAFLHWLIEQIKEHQVDALIVAGDIFDTGAPPSYAR
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
ELYNRFIVELQPTACQLVVLGGNHDSVATLNESRDLLSYLNTSVISCASRDLKQQVIILK
HHHHHHHEEECCCEEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
NRQQQPAAVLCAIPFLRPRDLVTSQAGESGDQKQLALQEAIAGHYQTLYQRALELRSQLG
CCCCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPLPIIATGHLTTVGVTTTDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQQVAKTEH
CCCCEEECCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IRYSGSPIALSFDELSKEKSVYLVEFDQQNLSSVTPLFIPQFQPMQLIKGDLRQIEQQLA
EEECCCCEEEEHHHHCCCCCEEEEEECCCCHHCCCCHHCCCCCHHHHHHHHHHHHHHHHH
AFAGHEGLPVWLDIEVATQDYLTDIQRRIQALAANLPVEVVLLRRSKEQRNNSIHRQEKE
HHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCHHHHHHHH
TLNELTVSDVFERRLALEADLSEPRQQRMRQMFNYVVDEIAQDGSNGVAEEPAQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRIIHTSDWHLGQYFFTKSRAAEHQAFLHWLIEQIKEHQVDALIVAGDIFDTGAPPSYAR
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
ELYNRFIVELQPTACQLVVLGGNHDSVATLNESRDLLSYLNTSVISCASRDLKQQVIILK
HHHHHHHEEECCCEEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
NRQQQPAAVLCAIPFLRPRDLVTSQAGESGDQKQLALQEAIAGHYQTLYQRALELRSQLG
CCCCCCCEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPLPIIATGHLTTVGVTTTDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQQVAKTEH
CCCCEEECCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IRYSGSPIALSFDELSKEKSVYLVEFDQQNLSSVTPLFIPQFQPMQLIKGDLRQIEQQLA
EEECCCCEEEEHHHHCCCCCEEEEEECCCCHHCCCCHHCCCCCHHHHHHHHHHHHHHHHH
AFAGHEGLPVWLDIEVATQDYLTDIQRRIQALAANLPVEVVLLRRSKEQRNNSIHRQEKE
HHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCHHHHHHHH
TLNELTVSDVFERRLALEADLSEPRQQRMRQMFNYVVDEIAQDGSNGVAEEPAQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]