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The map label for this gene is yfiF

Identifier: 51595204

GI number: 51595204

Start: 1030634

End: 1031806

Strand: Direct

Name: yfiF

Synonym: YPTB0854

Alternate gene names: 51595204

Gene position: 1030634-1031806 (Clockwise)

Preceding gene: 51595198

Following gene: 51595214

Centisome position: 21.72

GC content: 53.2

Gene sequence:

>1173_bases
ATGAACGATTCATTTAGTGGCAAAAACGGCAAAGTTAAAGTGATGTACGTCCGTAGTGACGAAAGCAGCGACAACCGTAA
CAGCAGTAACCGCGATAACCGCAACCGCGATAACAGTAGTCGTGATAACAGTAATCGTGATAATAACAGCCGCGGCAGAA
GCCAGCGTCCAGCGGGTAACGGTCGTCCGGGTGATAATGCACGTCAGGGCGATAATACACGTCAGGGTAATGCACCACGC
AATAATGACTCACGTCGTCCTGATTCTGGCCGTAATGAACGTGATCGTCCTAGCCGTCCGGCACGTTCTGAAGGCAGTAG
CCCTTACGATTCACCGTGGAAAACGGTATCTCGTGCCCCTTCGGAAGAGCCAGAGTTTGATCATGGTGGTATCAGCGGTA
AAAGCCATATCGATCCTGCCCAATTGCGCCGTCAGCGCGCTGAAGAAACACGCGTTTATGGTGAGAATGCTTGTAAGGCG
CTGTTTGAAAGCCGCCCTGATGCTATCGTCCGGGCTTGGTTTGTGCAATCTGTGACCCCGCGTTTTCGTGAAGCGCTGAA
ATGGATGGCGGCTAACCGCAAAGCTTATCATGTGGTTGAAGAGGATGAGCTGGCGAAAGCTTCGGGCACTGAACACCATG
GCGGGGTATGTTTCCTGATTAAAAAACGTCAGGGGCTGGATGCAGAAACGTATCTGCAACAGCAAACTCAGGCTAAAGAT
TGTGTACTGGCGCTGGAAGAGGTGGGTAACCCCCATAATTTGGGCGGCATTATGCGTACCTGTGCGCATTTCGGTATCAA
CGGCGTGTTGCTGCAAGACCCGGCGATGCTAGAATCTGGCGCGGCAGTCCGCACGGCGGAAGGCGGAGCAGAGCACATCA
AGGCCATCAATGCTGATGATTTCTTATCGGTATTGGATACCTTCCGCAAGGCGGGTTACACCATCGTGACCACCTCCAGC
CATAAAGGTGTCAGCTTATCGAAAGCCGAACTGCCAGCCAAAATGGTATTGGTATTAGGGCAGGAGAGTGATGGTTTGAC
GGACAGTGCCTGGCAGCAGGGGGATCTGAGTGTCTCTATCGGCGGTACCGGGCGGGTCGAAAGCCTGAACGTTTCCGTGG
CAACGGGTATCCTGTTGGCGGAATGGTGGCGTCAGAATAGCGCTCAAGGCTAG

Upstream 100 bases:

>100_bases
ATTCGATGAGTACGATAGTTGGTAGCTTTTAGTGCTTGCTTATGGCTAACCGGGCGGACATCAGGTAATCTTCGCGCCCT
GCGCGTTGGTGGAGAAGACA

Downstream 100 bases:

>100_bases
AATGATCGAAGAGTAGAACGAGCGACGTTTTAGAACGGTAAAAATAAAAGGGGGCGCTGATGAGTTCAGGGCCCCCTTTT
TTGTTGACCGCTATTTGGCT

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNDSFSGKNGKVKVMYVRSDESSDNRNSSNRDNRNRDNSSRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQGNAPR
NNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKA
LFESRPDAIVRAWFVQSVTPRFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKD
CVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTIVTTSS
HKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG

Sequences:

>Translated_390_residues
MNDSFSGKNGKVKVMYVRSDESSDNRNSSNRDNRNRDNSSRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQGNAPR
NNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKA
LFESRPDAIVRAWFVQSVTPRFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKD
CVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTIVTTSS
HKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG
>Mature_390_residues
MNDSFSGKNGKVKVMYVRSDESSDNRNSSNRDNRNRDNSSRDNSNRDNNSRGRSQRPAGNGRPGDNARQGDNTRQGNAPR
NNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAPSEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKA
LFESRPDAIVRAWFVQSVTPRFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKD
CVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADDFLSVLDTFRKAGYTIVTTSS
HKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSIGGTGRVESLNVSVATGILLAEWWRQNSAQG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=390, Percent_Identity=59.7435897435897, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1790623, Length=246, Percent_Identity=26.8292682926829, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 42759; Mature: 42759

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSFSGKNGKVKVMYVRSDESSDNRNSSNRDNRNRDNSSRDNSNRDNNSRGRSQRPAGN
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GRPGDNARQGDNTRQGNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
SEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALFESRPDAIVRAWFVQSVTP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCH
RFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKD
HHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
CVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADD
HHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCEEEECCCCHHHHEECCHHH
FLSVLDTFRKAGYTIVTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSI
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCCEEEEE
GGTGRVESLNVSVATGILLAEWWRQNSAQG
CCCCCEEEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDSFSGKNGKVKVMYVRSDESSDNRNSSNRDNRNRDNSSRDNSNRDNNSRGRSQRPAGN
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GRPGDNARQGDNTRQGNAPRNNDSRRPDSGRNERDRPSRPARSEGSSPYDSPWKTVSRAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
SEEPEFDHGGISGKSHIDPAQLRRQRAEETRVYGENACKALFESRPDAIVRAWFVQSVTP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCH
RFREALKWMAANRKAYHVVEEDELAKASGTEHHGGVCFLIKKRQGLDAETYLQQQTQAKD
HHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
CVLALEEVGNPHNLGGIMRTCAHFGINGVLLQDPAMLESGAAVRTAEGGAEHIKAINADD
HHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCEEEECCCCHHHHEECCHHH
FLSVLDTFRKAGYTIVTTSSHKGVSLSKAELPAKMVLVLGQESDGLTDSAWQQGDLSVSI
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCHHHHCCCCEEEEE
GGTGRVESLNVSVATGILLAEWWRQNSAQG
CCCCCEEEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]