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The map label for this gene is lpdA [H]

Identifier: 51594476

GI number: 51594476

Start: 139942

End: 141405

Strand: Reverse

Name: lpdA [H]

Synonym: YPTB0118

Alternate gene names: 51594476

Gene position: 141405-139942 (Counterclockwise)

Preceding gene: 51594477

Following gene: 51594467

Centisome position: 2.98

GC content: 51.43

Gene sequence:

>1464_bases
ATGAAAACCTTAAACGTTGATGTCGCTGTTATTGGCGGCGGAACGGCCGGACTGGGCGCTTATCGCGCGGCCAAACTGTT
GACCCCCAACGTAGTGATGATTGAAGGTGGTGAATATGGTACCACCTGCGCACGCGTCGGCTGCATGCCATCCAAACTAT
TGATTGCGGCGGCTGAAGCCGTCCACCAGATAGAAAAAGCGCCCGGATTTGGTATTCACCCACAAGGTAAGCCCCTGATT
AATGGCCGTGAAGTCATGGACCGCGTTAAGCGTGAACGTGACCGTTTTGTGGGCTTCGTATTGAAAGGCGTAGAGACCAT
TCCTGCGGCCGATAAAATTCAAGGTTATGCCCGTTTTATTGATGATAACACCCTGCAAGTTGATGATCACACGCAGCTTA
CTTCACAAAAAATCATTGCGCAGCGGATCGTGATTGCGACAGGTTCCCACCCAAGCTGGCCCGCCTCTTGGAATGAGCTA
GGTGACCGGTTGATTATTAATGATGACGTCTTTAATTGGGACGATCTGCCAGAATCTGTGGCAGTTTTTGGCCCAGGCGT
TATTGGCTTAGAACTTGGTCAGGCACTGCACCGGTTAGGTGTCCAGGTAAAAATGTTTGGTGTCGGTGGTGCCGTTGGCC
CGCTGACCGACAGTATCGTGCGTGACTACGCAGCAAAAACGTTGGGTGATGAGTTCTATCTTGATCCCGATGTAAAAGTT
GAGCTGATGCAACGTGAGGGAGATAAAGTCTTTATCCGTTATCTGGATAAAAGCGGTAGGCCACAGGAGATTATGGTGGA
CTACGTCTTAGCCGCCACGGGCCGCCGCCCTAATGTAGATAAATTGGGGCTGGAGAATACGTCACTCATTCTGGATGAAC
GCGGAGTGCCGCAGGCAGACCGCCTGACCATGCAAACCAACGTGCCGCATATTTTTATTGCAGGTGATGCCAGCAATCAG
TTGCCATTACTGCATGAAGCCAGCGATCAGGCACGTATTGCCGGTGTAAATGCGGGAGGATTCCCTGAGGTTGTACCCGG
TTTACGCCGTAGCCCAATTTCAGTCGTGTTCTCTGACCCACAAATTGCCATGGTGGGGGCAACCTTCCGTGAGCTAGCAC
AAAAATTCAGCGCTTGTGGTTGCTTTGAGATTGGCGAAGTCTCCTTTGAGAATCAAGGTCGCTCGCGGGTGATGCTAAAA
AACAAAGGCATTTTACGCATTTATGGCGAACAGGGCACTGGCCGATTCTTAGGGGCAGAGATGATGGGGCCAAGTGCGGA
ACACATCGCCCACTTACTGGCATGGGCGCATCAGCAACAAATGACCATCGACCAGATGCTCGATATGCCGTTTTATCATC
CGGTCATTGAAGAAGGCCTGCGTACCGCATTGCGTGATTTGCAATCTAAATTAAAGCTGGGCGCTGATGAAGCGGAGCGC
TGCCTACGCTGCCCTGGTGAATAA

Upstream 100 bases:

>100_bases
AGCCTAATTATGTTGATTAACAAACATATTAATAAAAATTAAAGCAATAGGAAGTAACTTGGTAGGCTCCGGCCTACCTT
TTTTTCTGTTAGGAGTAGAC

Downstream 100 bases:

>100_bases
GGCCTCAGGCGGTTCAAACCGGGCACGACGCCCGGTTTTTAATCCCCCGTTCTCCCCTCTCTTATTTTGCGTATTTTGCG
CTCTACTCATATGTTACCCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEAVHQIEKAPGFGIHPQGKPLI
NGREVMDRVKRERDRFVGFVLKGVETIPAADKIQGYARFIDDNTLQVDDHTQLTSQKIIAQRIVIATGSHPSWPASWNEL
GDRLIINDDVFNWDDLPESVAVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQADRLTMQTNVPHIFIAGDASNQ
LPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDPQIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLK
NKGILRIYGEQGTGRFLGAEMMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
CLRCPGE

Sequences:

>Translated_487_residues
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEAVHQIEKAPGFGIHPQGKPLI
NGREVMDRVKRERDRFVGFVLKGVETIPAADKIQGYARFIDDNTLQVDDHTQLTSQKIIAQRIVIATGSHPSWPASWNEL
GDRLIINDDVFNWDDLPESVAVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQADRLTMQTNVPHIFIAGDASNQ
LPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDPQIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLK
NKGILRIYGEQGTGRFLGAEMMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
CLRCPGE
>Mature_487_residues
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEAVHQIEKAPGFGIHPQGKPLI
NGREVMDRVKRERDRFVGFVLKGVETIPAADKIQGYARFIDDNTLQVDDHTQLTSQKIIAQRIVIATGSHPSWPASWNEL
GDRLIINDDVFNWDDLPESVAVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQADRLTMQTNVPHIFIAGDASNQ
LPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDPQIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLK
NKGILRIYGEQGTGRFLGAEMMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
CLRCPGE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=27.6150627615063, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI50301238, Length=468, Percent_Identity=25.4273504273504, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI148277065, Length=460, Percent_Identity=22.6086956521739, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI148277071, Length=460, Percent_Identity=22.6086956521739, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI33519430, Length=460, Percent_Identity=22.6086956521739, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI33519428, Length=460, Percent_Identity=22.6086956521739, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI33519426, Length=460, Percent_Identity=22.6086956521739, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786307, Length=469, Percent_Identity=24.9466950959488, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=27.5210084033613, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789915, Length=379, Percent_Identity=28.4960422163588, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=24.5202558635394, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32565766, Length=477, Percent_Identity=27.6729559748428, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17557007, Length=305, Percent_Identity=25.2459016393443, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71983429, Length=371, Percent_Identity=24.7978436657682, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71983419, Length=371, Percent_Identity=24.7978436657682, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=27.4261603375527, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325166, Length=395, Percent_Identity=28.3544303797468, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6325240, Length=349, Percent_Identity=25.7879656160458, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21358499, Length=477, Percent_Identity=27.6729559748428, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=26.7748478701826, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24640549, Length=494, Percent_Identity=26.5182186234818, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24640551, Length=486, Percent_Identity=26.5432098765432, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=23.7322515212982, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 53132; Mature: 53132

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEA
CCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPGFGIHPQGKPLINGREVMDRVKRERDRFVGFVLKGVETIPAADKIQGYARFI
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DDNTLQVDDHTQLTSQKIIAQRIVIATGSHPSWPASWNELGDRLIINDDVFNWDDLPESV
CCCEEEECCCHHHHHHHHHHHEEEEEECCCCCCCCCHHHCCCEEEEECCCCCCCCCCCHH
AVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
HHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEE
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQAD
EEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHH
RLTMQTNVPHIFIAGDASNQLPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDP
CEEEECCCCEEEEECCCCCCCCEEECCCCCCEEEECCCCCCHHHCCCCCCCCEEEEECCC
QIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLKNKGILRIYGEQGTGRFLGAE
CEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEHH
MMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
CLRCPGE
HHCCCCC
>Mature Secondary Structure
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEA
CCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPGFGIHPQGKPLINGREVMDRVKRERDRFVGFVLKGVETIPAADKIQGYARFI
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DDNTLQVDDHTQLTSQKIIAQRIVIATGSHPSWPASWNELGDRLIINDDVFNWDDLPESV
CCCEEEECCCHHHHHHHHHHHEEEEEECCCCCCCCCHHHCCCEEEEECCCCCCCCCCCHH
AVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
HHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEE
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQAD
EEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHH
RLTMQTNVPHIFIAGDASNQLPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDP
CEEEECCCCEEEEECCCCCCCCEEECCCCCCEEEECCCCCCHHHCCCCCCCCEEEEECCC
QIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLKNKGILRIYGEQGTGRFLGAE
CEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEHH
MMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
CLRCPGE
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]