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The map label for this gene is trmL [H]

Identifier: 51594425

GI number: 51594425

Start: 78059

End: 78547

Strand: Reverse

Name: trmL [H]

Synonym: YPTB0067

Alternate gene names: 51594425

Gene position: 78547-78059 (Counterclockwise)

Preceding gene: 51594427

Following gene: 51594418

Centisome position: 1.66

GC content: 49.08

Gene sequence:

>489_bases
ATGCTTAACATTGTTTTATTTGAACCCGAAATTCCGCCAAATACCGGCAATATTATCCGGTTATGTGCGAATACCGGCTG
CCAGCTTCATCTGATTAAACCTTTGGGTTTTACTTGGGACGATAAACGCTTACGCCGTGCGGGCCTTGATTACCATGAGT
TTGCCGACATCAAACATCACCATGATTATCAAGCCTTCCTGGACAGTGAAAAACTCGATAGCACACAACTGGCTCGCTTA
TTTGCGCTGACGACCAAGGGGACGCCGGCTCACAGTGCGGTGAGCTATCAGGCGAATGATTACCTGTTATTTGGCCCAGA
AACCCGTGGCTTACCGGCCTATATTCTGGATGCGTTACCCGCTCAGCAGAAAATCAGAATCCCCATGCAAGCCGATAGCC
GGAGCATGAATCTATCGAATGCCGTTTCAGTCGTGGTGTATGAGGCCTGGCGTCAACTGGGCTATCCAGGTGCATTACTG
AAAGAGTGA

Upstream 100 bases:

>100_bases
CTCCTTGCTTTTAAATTTTTGTTTTACACCGCCGCTACTGGCAACCGCTGCACTGAACAAGGGTTTCTGGTATCCTGCGC
CCCTCATTATTGGGGGACAT

Downstream 100 bases:

>100_bases
TTTTTCTTACGCAGAGAGAACGTGTCAGCCACAATCAGAGTGGCTAATCTGCCCCATATTAGGGGCATATCCAAAACAGT
TGGCGCTGGCGTTGCAGCAA

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHHHDYQAFLDSEKLDSTQLARL
FALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALPAQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALL
KE

Sequences:

>Translated_162_residues
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHHHDYQAFLDSEKLDSTQLARL
FALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALPAQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALL
KE
>Mature_162_residues
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHHHDYQAFLDSEKLDSTQLARL
FALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALPAQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALL
KE

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=162, Percent_Identity=79.6296296296296, Blast_Score=272, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18250; Mature: 18250

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHH
CEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHCCCCC
HDYQAFLDSEKLDSTQLARLFALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALP
CCHHHHHCCCCCCHHHHHHHHEEECCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHCCC
AQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALLKE
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MLNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHH
CEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHCCCCC
HDYQAFLDSEKLDSTQLARLFALTTKGTPAHSAVSYQANDYLLFGPETRGLPAYILDALP
CCHHHHHCCCCCCHHHHHHHHEEECCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHCCC
AQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGYPGALLKE
CCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360 [H]