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The map label for this gene is trmH

Identifier: 51594392

GI number: 51594392

Start: 47023

End: 47715

Strand: Reverse

Name: trmH

Synonym: YPTB0034

Alternate gene names: 51594392

Gene position: 47715-47023 (Counterclockwise)

Preceding gene: 51594393

Following gene: 51594391

Centisome position: 1.01

GC content: 49.64

Gene sequence:

>693_bases
ATGAATCCTCAACGCTATGCGCGGATTTGTGACATGCTTGCCACCAGGCAACCCGATTTAACGGTCTGTCTGGAGCAAGT
CCACAAACCTCATAATGTCTCCGCCATTATTCGAACTGCCGATGCCGTGGGCATCCATCAAGTTCATGCCATCTGGCCAA
CAACGCAAATGTATACTCGGCTGTCCGCCGCCGCAGGCAGTAATAGCTGGGTGCAAGTCAAAACGCATCCCCATATTGCT
GATGCCATTGCTTATCTAAAATCACAAGATATGCAAATTCTGGCGACACATCTGTCTGATAAGGCTGTTGATTTTCGTGA
AATCGATTATACCCGCCCTACCTGTATTTTAATGGGTCAGGAAAAAACCGGTATTTCACCAGAAGCGCTGGCACTGGCCG
ATCAAGACATCATCATTCCAATGATCGGCATGGTACAGTCGCTGAATGTTTCCGTCGCTTCCGCGCTAATTTTATATGAA
GCACAGCGGCAACGGCAGAATGCCGGGATGTATAAACGGACACACAGCGTTCTGGCAGAGAGTGAGCAGCAACGGCTATT
GTTTGAAGGGGGTTACCCGGTATTGGCACACGTTGCCAAACGTAAAGGGCTCCCCCAACCTCATATTGATGAACAAGGCC
AAATCATTGCCGATGCGCAATGGTGGTCGGCCATGCAAGCCACGGAGTCTTAA

Upstream 100 bases:

>100_bases
GCCTGACCACTCGGAACCGGGTCCATCTGGCTAACATTATGCGTAAAATCCGTATCATGCCAGATGTGGTTAAAGTTAGC
CGTAATCGCAATTAACGCCT

Downstream 100 bases:

>100_bases
ATGAAAGGCCGCCTACTGGACGCCGTACCCCTCAGTACACTTTCCGGAGTTGGCGCAAGTCAGGCTGGAAAACTCGCCAA
GATAGGTTTGGAAACCATTC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MNPQRYARICDMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWVQVKTHPHIA
DAIAYLKSQDMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYKRTHSVLAESEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES

Sequences:

>Translated_230_residues
MNPQRYARICDMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWVQVKTHPHIA
DAIAYLKSQDMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYKRTHSVLAESEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES
>Mature_230_residues
MNPQRYARICDMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTRLSAAAGSNSWVQVKTHPHIA
DAIAYLKSQDMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYKRTHSVLAESEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=227, Percent_Identity=82.8193832599119, Blast_Score=395, Evalue=1e-112,
Organism=Escherichia coli, GI1790623, Length=159, Percent_Identity=32.0754716981132, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25632; Mature: 25632

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQRYARICDMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTR
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
LSAAAGSNSWVQVKTHPHIADAIAYLKSQDMQILATHLSDKAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCCCCCEEEEEEC
EKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYKRTHSVLAE
CCCCCCCHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
SEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES
CHHHEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHCCC
>Mature Secondary Structure
MNPQRYARICDMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGIHQVHAIWPTTQMYTR
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
LSAAAGSNSWVQVKTHPHIADAIAYLKSQDMQILATHLSDKAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCCCCCEEEEEEC
EKTGISPEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYKRTHSVLAE
CCCCCCCHHHEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
SEQQRLLFEGGYPVLAHVAKRKGLPQPHIDEQGQIIADAQWWSAMQATES
CHHHEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]