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The map label for this gene is glnG

Identifier: 51594380

GI number: 51594380

Start: 29784

End: 31196

Strand: Reverse

Name: glnG

Synonym: YPTB0022

Alternate gene names: 51594380

Gene position: 31196-29784 (Counterclockwise)

Preceding gene: 51594381

Following gene: 51594377

Centisome position: 0.66

GC content: 52.72

Gene sequence:

>1413_bases
ATGCAACGAGGGATAGTCTGGATCGTCGATGACGATAGTTCCATCCGCTGGGTGCTTGAGCGCGCACTGACTGGAGCGGG
TCTAAACTGTGTCGCATTCGATAGCGGCAATCAGGTATTGGATGCACTGGCTACGCGAACTCCAGATGTGTTGTTATCAG
ATATCCGTATGCCAGGAATGGATGGCTTGGCATTACTCAAACAAATTAAGCAACGCCATCCGATGCTCCCGGTCATCATA
ATGACGGCCCATTCAGATTTAGATGCCGCCGTCAGTGCGTATCAGCAAGGGGCCTTTGACTATTTGCCCAAACCGTTTGA
TATCGACGAGGCCGTGGCTCTGGTTGAACGGGCTATTAGCCATTATCAGGAGCAACAACAACCCGCCCGTAGCCAACCAG
TCAGTGGGCCGACAGCCGATATTATCGGCGAAGCACCGGCGATGCAGGATGTTTTTCGTATCATTGGCCGCTTGTCACGC
TCCTCAATTAGCGTACTGATTAATGGTGAATCCGGTACCGGTAAAGAGCTGGTCGCACACGCATTACATCGCCACAGCCC
ACGGGCTAAAGCCCCCTTTATCGCACTGAACATGGCGGCTATCCCGAAAGATTTAATTGAGTCGGAACTGTTCGGTCACG
AAAAAGGGGCATTTACCGGGGCCAATCAGGTACGGCAGGGGCGCTTTGAACAAGCTGACACCGGCACACTTTTTTTAGAT
GAAATTGGTGATATGCCACTGGATGTGCAAACCCGCCTGTTACGGGTATTGGCTGATGGCCAGTTCTATCGGGTCGGGGG
CTATGCACCCGTTAAAGTCGATGTGCGGATCATTGCGGCAACTCACCAGAATCTGGAGTTGCGTGTTCAAGAAGGAAAAT
TCCGTGAGGATCTATTCCATCGTCTGAATGTGATCCGAGTACATTTACCTCCACTACGTGAACGCAGTGAGGATATTCCG
CGTTTGGCTCGCTACTTTTTACACGTAGCGGCTAAAGAGTTGGGCGTGGAAAGCAAAAATCTGCATCCAGAGACCGAAGC
GGCGCTAACCCGCTTACCTTGGCCTGGGAACGTGCGCCAACTGGAGAATACCTGTCGTTGGCTGACCGTCATGGCCGCTG
GGCAGGAAGTCCTCGTTCAGGATCTGCCGTCTGAATTATTTGAAACTCATATGCCCGATGCATCCGGCCAACGAGCCCCC
GACAGTTGGTCAACGCTGTTAGCGCAATGGGCGGATCGTGCGTTGCGTTCTGGTCATCAGGATTTACTGTCTGAAGCACA
ACCAGAAATGGAGCGCACGCTGTTAACCACCGCATTACGCCATACCCAAGGCCATAAGCAGGAAGCAGCACGCCTGCTGG
GCTGGGGCCGTAATACATTAACGCGGAAGTTGAAAGAGTTGGGGATGGAGTAG

Upstream 100 bases:

>100_bases
CGTAATCTTATCGATCAGCATTCGGGTAAAATTGAATTTAACAGTTGGCCAGGACACACCGAGTTTTCGGTTTACCTGCC
TATTCGCCAGTGAGGTTTCT

Downstream 100 bases:

>100_bases
TTCCCGCCAGATGGTCACCCTCAAGAGCGATAACCGCACTGAGGGTGATATCGTCAGATTACACGTGAGAATTGCTGCTT
GCGCGCCTGTTTACGTAAAT

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MQRGIVWIVDDDSSIRWVLERALTGAGLNCVAFDSGNQVLDALATRTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARSQPVSGPTADIIGEAPAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANQVRQGRFEQADTGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERSEDIP
RLARYFLHVAAKELGVESKNLHPETEAALTRLPWPGNVRQLENTCRWLTVMAAGQEVLVQDLPSELFETHMPDASGQRAP
DSWSTLLAQWADRALRSGHQDLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Sequences:

>Translated_470_residues
MQRGIVWIVDDDSSIRWVLERALTGAGLNCVAFDSGNQVLDALATRTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARSQPVSGPTADIIGEAPAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANQVRQGRFEQADTGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERSEDIP
RLARYFLHVAAKELGVESKNLHPETEAALTRLPWPGNVRQLENTCRWLTVMAAGQEVLVQDLPSELFETHMPDASGQRAP
DSWSTLLAQWADRALRSGHQDLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
>Mature_470_residues
MQRGIVWIVDDDSSIRWVLERALTGAGLNCVAFDSGNQVLDALATRTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPARSQPVSGPTADIIGEAPAMQDVFRIIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANQVRQGRFEQADTGTLFLD
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERSEDIP
RLARYFLHVAAKELGVESKNLHPETEAALTRLPWPGNVRQLENTCRWLTVMAAGQEVLVQDLPSELFETHMPDASGQRAP
DSWSTLLAQWADRALRSGHQDLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=90.4255319148936, Blast_Score=873, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=42.0940170940171, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1790437, Length=461, Percent_Identity=41.8655097613883, Blast_Score=347, Evalue=9e-97,
Organism=Escherichia coli, GI1788905, Length=459, Percent_Identity=37.9084967320261, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=44.0729483282675, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=40.7932011331445, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1789087, Length=452, Percent_Identity=36.9469026548673, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1786524, Length=339, Percent_Identity=42.7728613569322, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI87082152, Length=334, Percent_Identity=39.8203592814371, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787583, Length=345, Percent_Identity=39.4202898550725, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=39.7590361445783, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI87081858, Length=414, Percent_Identity=29.2270531400966, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=34.3220338983051, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1790860, Length=129, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786911, Length=136, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=29.8387096774194, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789402, Length=111, Percent_Identity=32.4324324324324, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI2367329, Length=126, Percent_Identity=41.2698412698413, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52357; Mature: 52357

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRGIVWIVDDDSSIRWVLERALTGAGLNCVAFDSGNQVLDALATRTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPARSQPVSGPTADIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAH
HHHHHHCCHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANQVRQGRFEQADTGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECCCHHHHHHH
RLNVIRVHLPPLRERSEDIPRLARYFLHVAAKELGVESKNLHPETEAALTRLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHH
LENTCRWLTVMAAGQEVLVQDLPSELFETHMPDASGQRAPDSWSTLLAQWADRALRSGHQ
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHH
DLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRGIVWIVDDDSSIRWVLERALTGAGLNCVAFDSGNQVLDALATRTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPARSQPVSGPTADIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAH
HHHHHHCCHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANQVRQGRFEQADTGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEHH
EIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFH
HHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECCCHHHHHHH
RLNVIRVHLPPLRERSEDIPRLARYFLHVAAKELGVESKNLHPETEAALTRLPWPGNVRQ
HHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHH
LENTCRWLTVMAAGQEVLVQDLPSELFETHMPDASGQRAPDSWSTLLAQWADRALRSGHQ
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHH
DLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]