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The map label for this gene is leuB

Identifier: 161760590

GI number: 161760590

Start: 802372

End: 803463

Strand: Reverse

Name: leuB

Synonym: YPTB0671

Alternate gene names: 161760590

Gene position: 803463-802372 (Counterclockwise)

Preceding gene: 51595022

Following gene: 51595020

Centisome position: 16.93

GC content: 50.82

Gene sequence:

>1092_bases
ATGACGAAGACTTATCATATTGCAGTGTTGCCCGGCGACGGTATCGGCCCTGAAGTAATGGCTCAGGCAAGTAAAGTATT
GGATGCGGTGCGTCAGCGCTTCGGTCTCAAGATTTCAACCAGTGTTTACGATGTTGGTGGCGCTGCCATTGACCGCCATG
GTAGCCCATTACCGGCAGCAACGGTCGCGGGATGTGAACAGGCAGATGCCATTTTATTTGGCTCCGTAGGCGGCCCAAAA
TGGGAACACCTGCCACCGGCAGAACAACCTGAGCGTGGTGCCTTATTGCCTTTGCGTAAACATTTCAAATTATTCAGCAA
TTTGCGCCCTGCCCGTTTGTATCAAGGATTGGAGGATTTCTGTCCATTGCGCAGTGATATCGCGGCCAGAGGTTTTGATA
TCCTGTGTGTCCGTGAATTAACCGGCGGTATCTATTTCGGCCAGCCGAAAGGCCGTGAAGGCCAAGGCATGCACGAGCGG
GCCTTTGACACCGAAGTTTATCATCGCTTTGAAATTGAACGTATCGCCCGTATTGCTTTCGAATCAGCCCGCAAACGCCG
TAGTAAAGTAACGTCCATTGATAAAGCCAACGTGTTGCAAAGTTCAATCTTGTGGCGTGAAGTGGTCAACGGGATCGCGG
CGGATTACCCAGATGTTGCGTTGTCCCATATGTACATTGATAACGCCACCATGCAGTTAATCAAAGACCCTTCCCAATTT
GACGTGTTGCTGTGCTCTAACTTGTTTGGCGATATCTTGTCCGATGAATGCGCCATGATCACCGGCTCCATGGGCATGTT
GCCATCAGCCAGTTTGAATGAACAAGGTTTTGGTCTATATGAACCCGCAGGCGGTTCCGCACCGGATATCGCGGGTAAGA
ACATTGCCAACCCTATCGCTCAGATCCTGTCATTAATCCTGTTGCTGCGTTTTAGCTTAGGTAAAGATGATGCCGCTGAC
GCCATTGAGCGCGCGATCAACCAGGCATTGGAGCAAGGTTATCGCACTGCGGATTTAGCCGGGGATGGTCACGCAATCGG
CACTCACGAAATGGGCGATATCATCGCTAAATTCGTGGTTGAGGGGGTATAA

Upstream 100 bases:

>100_bases
CTAAGGCGTTGGTTCACGTATTAAATAACATCTGGCGCGCCCATCAGGTCGAAAAAGAGAAGCAGCGTCTGCAACAAAAT
AATCAGGAAATGGTGTGAAC

Downstream 100 bases:

>100_bases
AATGGGCACAACATCATCTCAGAGCAAAACGTTATACCAAAAATTGTACGATGCGCACATTGTGCATGAAGCCCCCAATG
AGACCCCATTGCTGTATATC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHER
AFDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLILLLRFSLGKDDAAD
AIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV

Sequences:

>Translated_363_residues
MTKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHER
AFDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLILLLRFSLGKDDAAD
AIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV
>Mature_362_residues
TKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPKW
EHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERA
FDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQFD
VLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLILLLRFSLGKDDAADA
IERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=33.4310850439883, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4758582, Length=357, Percent_Identity=28.2913165266106, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI28178821, Length=315, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI28178816, Length=315, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178838, Length=331, Percent_Identity=26.5861027190332, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=36.6666666666667, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=85.9504132231405, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI1788101, Length=371, Percent_Identity=38.544474393531, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=26.1306532663317, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=32.7485380116959, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=30.1136363636364, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=343, Percent_Identity=30.3206997084548, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=345, Percent_Identity=28.9855072463768, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=43.2432432432432, Blast_Score=268, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=33.8068181818182, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=30.835734870317, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=344, Percent_Identity=29.6511627906977, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=32.8445747800587, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=32.8445747800587, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=29.3604651162791, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=29.7935103244838, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=29.7935103244838, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=29.4985250737463, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=29.4985250737463, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=29.673590504451, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=26.7647058823529, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=26.7647058823529, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=24.4186046511628, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39279; Mature: 39148

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAA
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCEECCCCHHHCCCCCCCCHH
TVAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHH
CPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
QILSLILLLRFSLGKDDAADAIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
EGV
CCC
>Mature Secondary Structure 
TKTYHIAVLPGDGIGPEVMAQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCEECCCCHHHCCCCCCCCHH
TVAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHH
CPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRSKVTSIDKANVLQSSILWREVVNGIAADYPDVALSHMYIDNATMQLIKDPSQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
QILSLILLLRFSLGKDDAADAIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
EGV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]