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The map label for this gene is dnaB [H]

Identifier: 50843678

GI number: 50843678

Start: 2427460

End: 2428806

Strand: Direct

Name: dnaB [H]

Synonym: PPA2244

Alternate gene names: 50843678

Gene position: 2427460-2428806 (Clockwise)

Preceding gene: 50843673

Following gene: 50843679

Centisome position: 94.81

GC content: 59.84

Gene sequence:

>1347_bases
ATGTCCAGCGCACCCGCCGAGCCGGGCCCGTCCTTGGACCGTAACCCTCCCCAGGACATTGACGCCGAAAAGTCCGTACT
AGGTGCCATGTTGTCTTCCAAAGACGCCATCGCCGACGTCGTCGAAGAGATCAAGGGCGTCGATTTCTACCGCCCTGGAC
ACGAGCTCATCTTCAACACCATCACCGATCTATACGGTCGCGGCGACCCTGCCGACACCGTCACCACCGCCGATGAACTA
GATCGCCGCGGCGAACTCGAGCGAGCCGGCGGACGCCTGTACCTCGCAGAATTGCTAACCAATGTCACCGTCACCGCGAA
CGCGGCATACTACGCCAAGATTGTCGCCGAGAAAGCCGTTCTTCGTCGCCTCGTCGAAGCGTCAATCCGCATCGCTCAGA
TGGGCTACCAGGGACAAGGAGAGGTCGCCGACATCGTCGACGCCGCCCAGCAGACCCTCTACGACGTCTCCACCCGCAAA
ACCAGTGAGGAATACCACCCACTCTCGGAATTATTCGAGAGCACCTTTGACGAGCTTGAGGCCATTGAGGCTCGCGGCGA
CGCCATGGCCGGCATTCCTACTGGGTTCACCGACCTTGATGAGCTCACCAACGGGTTCATGCCCGGCCAGATGATCATCG
TCGCCGCCCGTCCGGCCATGGGAAAATCCACTCTGGCCCTCGACTTTGCCCGCGCCGCCGCGATCAAGAACCATCTCGCA
GCTGCAATCTTCAGCCTCGAGATGGGGCGCAACGAGATCGTCATGAGATTGCTATCTGCCGAGGCAGGGATCGAGCTGTC
CCGGATGCGTGCGGGGCGCCTGTCCGAAGAGGATTGGCAGCGCATGGTCGACAAAACAACACAGATCTCGTCAGCACCGT
TGTTCATCGATGACTCCCCTAACCTGACGATGATGGAAATCCGCGCCAAAGCAAGGCGGATGAAGCAAAAATTCGACCTC
AAGCTCGTCATCATCGATTACATGCAGCTCATGACCTCCGGCAAAAAGGTGGAGTCGCGTCAGCTGGAAGTCTCGGAGTT
TTCTCGCCAGATCAAACTGTTGGCTAAGGAGCTCGAGATCCCTGTCGTGGCCCTTTCCCAGCTCAACCGTGGCCCCGAGC
AGCGCACCGACAAGAAACCCATGATGAGTGACCTGCGCGAGTCCGGGTCACTCGAGCAGGACGCTGACGTCGTCATCCTG
TTGCACCGAGAGGACGTTTACGACAAAGAGTCCCCGCGCGCCGGCGAGGCCGATATCATCGTCGCCAAGCACCGCAACGG
GCCGACACGGACCGTGCCAGTGGTGTTCCAAGGCCACTACTCGCGTTTCGTCGACATGCAGCGCTAA

Upstream 100 bases:

>100_bases
ATCCATCCAGGCATTACCCACAAGACCTGTGGATAACTCTTATCCGGGAGTACTTAAAAACGTGGCACACTAGCCCCCTG
TCAGCCGTGAAGGAGCCGCC

Downstream 100 bases:

>100_bases
CAGCGGTACCCAAGCAACCCGGTCGGCACTACCGTGGGGTCATGGAAAGCATGCACGCCGTCGCCATCACCGGGCCCCTG
CCGGTCTCCGATCCCGAATG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSSAPAEPGPSLDRNPPQDIDAEKSVLGAMLSSKDAIADVVEEIKGVDFYRPGHELIFNTITDLYGRGDPADTVTTADEL
DRRGELERAGGRLYLAELLTNVTVTANAAYYAKIVAEKAVLRRLVEASIRIAQMGYQGQGEVADIVDAAQQTLYDVSTRK
TSEEYHPLSELFESTFDELEAIEARGDAMAGIPTGFTDLDELTNGFMPGQMIIVAARPAMGKSTLALDFARAAAIKNHLA
AAIFSLEMGRNEIVMRLLSAEAGIELSRMRAGRLSEEDWQRMVDKTTQISSAPLFIDDSPNLTMMEIRAKARRMKQKFDL
KLVIIDYMQLMTSGKKVESRQLEVSEFSRQIKLLAKELEIPVVALSQLNRGPEQRTDKKPMMSDLRESGSLEQDADVVIL
LHREDVYDKESPRAGEADIIVAKHRNGPTRTVPVVFQGHYSRFVDMQR

Sequences:

>Translated_448_residues
MSSAPAEPGPSLDRNPPQDIDAEKSVLGAMLSSKDAIADVVEEIKGVDFYRPGHELIFNTITDLYGRGDPADTVTTADEL
DRRGELERAGGRLYLAELLTNVTVTANAAYYAKIVAEKAVLRRLVEASIRIAQMGYQGQGEVADIVDAAQQTLYDVSTRK
TSEEYHPLSELFESTFDELEAIEARGDAMAGIPTGFTDLDELTNGFMPGQMIIVAARPAMGKSTLALDFARAAAIKNHLA
AAIFSLEMGRNEIVMRLLSAEAGIELSRMRAGRLSEEDWQRMVDKTTQISSAPLFIDDSPNLTMMEIRAKARRMKQKFDL
KLVIIDYMQLMTSGKKVESRQLEVSEFSRQIKLLAKELEIPVVALSQLNRGPEQRTDKKPMMSDLRESGSLEQDADVVIL
LHREDVYDKESPRAGEADIIVAKHRNGPTRTVPVVFQGHYSRFVDMQR
>Mature_447_residues
SSAPAEPGPSLDRNPPQDIDAEKSVLGAMLSSKDAIADVVEEIKGVDFYRPGHELIFNTITDLYGRGDPADTVTTADELD
RRGELERAGGRLYLAELLTNVTVTANAAYYAKIVAEKAVLRRLVEASIRIAQMGYQGQGEVADIVDAAQQTLYDVSTRKT
SEEYHPLSELFESTFDELEAIEARGDAMAGIPTGFTDLDELTNGFMPGQMIIVAARPAMGKSTLALDFARAAAIKNHLAA
AIFSLEMGRNEIVMRLLSAEAGIELSRMRAGRLSEEDWQRMVDKTTQISSAPLFIDDSPNLTMMEIRAKARRMKQKFDLK
LVIIDYMQLMTSGKKVESRQLEVSEFSRQIKLLAKELEIPVVALSQLNRGPEQRTDKKPMMSDLRESGSLEQDADVVILL
HREDVYDKESPRAGEADIIVAKHRNGPTRTVPVVFQGHYSRFVDMQR

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=434, Percent_Identity=40.5529953917051, Blast_Score=334, Evalue=9e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49713; Mature: 49582

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAPAEPGPSLDRNPPQDIDAEKSVLGAMLSSKDAIADVVEEIKGVDFYRPGHELIFNT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHH
ITDLYGRGDPADTVTTADELDRRGELERAGGRLYLAELLTNVTVTANAAYYAKIVAEKAV
HHHHHCCCCCCCCCCCHHHHHHCCCHHHCCCCEEHHHHHHCCEEEECHHHHHHHHHHHHH
LRRLVEASIRIAQMGYQGQGEVADIVDAAQQTLYDVSTRKTSEEYHPLSELFESTFDELE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHH
AIEARGDAMAGIPTGFTDLDELTNGFMPGQMIIVAARPAMGKSTLALDFARAAAIKNHLA
HHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AAIFSLEMGRNEIVMRLLSAEAGIELSRMRAGRLSEEDWQRMVDKTTQISSAPLFIDDSP
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
NLTMMEIRAKARRMKQKFDLKLVIIDYMQLMTSGKKVESRQLEVSEFSRQIKLLAKELEI
CEEHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PVVALSQLNRGPEQRTDKKPMMSDLRESGSLEQDADVVILLHREDVYDKESPRAGEADII
CHHHHHHHCCCCHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEE
VAKHRNGPTRTVPVVFQGHYSRFVDMQR
EEECCCCCCCEEEEEEECCHHHHHCCCC
>Mature Secondary Structure 
SSAPAEPGPSLDRNPPQDIDAEKSVLGAMLSSKDAIADVVEEIKGVDFYRPGHELIFNT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHH
ITDLYGRGDPADTVTTADELDRRGELERAGGRLYLAELLTNVTVTANAAYYAKIVAEKAV
HHHHHCCCCCCCCCCCHHHHHHCCCHHHCCCCEEHHHHHHCCEEEECHHHHHHHHHHHHH
LRRLVEASIRIAQMGYQGQGEVADIVDAAQQTLYDVSTRKTSEEYHPLSELFESTFDELE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHH
AIEARGDAMAGIPTGFTDLDELTNGFMPGQMIIVAARPAMGKSTLALDFARAAAIKNHLA
HHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AAIFSLEMGRNEIVMRLLSAEAGIELSRMRAGRLSEEDWQRMVDKTTQISSAPLFIDDSP
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
NLTMMEIRAKARRMKQKFDLKLVIIDYMQLMTSGKKVESRQLEVSEFSRQIKLLAKELEI
CEEHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PVVALSQLNRGPEQRTDKKPMMSDLRESGSLEQDADVVILLHREDVYDKESPRAGEADII
CHHHHHHHCCCCHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEE
VAKHRNGPTRTVPVVFQGHYSRFVDMQR
EEECCCCCCCEEEEEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA