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The map label for this gene is serS

Identifier: 50843654

GI number: 50843654

Start: 2404111

End: 2405385

Strand: Reverse

Name: serS

Synonym: PPA2219

Alternate gene names: 50843654

Gene position: 2405385-2404111 (Counterclockwise)

Preceding gene: 50843655

Following gene: 50843653

Centisome position: 93.95

GC content: 57.8

Gene sequence:

>1275_bases
ATGATCGATCCCAAGCTCCTGCGAACGGATCCGGACGCCGTTCGTCGCTCCCAGGCCGCCCGCGGCGAGGACTCCTCGGT
TGTGGACGACGTTGTCGCCGCAGATGAGGCTCGTCGTGAGGCTATTGCTGCCCATGAGAACCTGCGTGCAGAACAGAAGG
GACTCGGCAAGCGAATCGCTAAAGCATCCGGTGACGAGAAGGCGGAGTTGCTGTCCCGTACTAAGACGATTTCCGGTGAG
GTTGCTGACCTCAAGAAAGCCGCGGACGAGGCAGATGCCAAGTTTACCGAGCTTGCCAAGACTCTCGGCAATATCGTTAT
TGACGGGGTTCCTGCTGGTGGTGAGGATGAAGGTGTCATCAAGGAAACCGTCGGAACTCCCCGCGATTTTGCCGCTGAAG
GGTTCGAGCCTAAGGATCACCTCGAGATCGGTGAAGCCCTCGGCGCTATCGATATGGAGCGCGGCACAAAGATTTCTGGA
TCCCGGTTCTACGTCCTCACCGGCGTTGGCGCTCAGTTGGAATTCGCGCTGCTCAACCTAGCGATGTCTAAAGCGGCTCA
GTGGGGATTCACCCCCATGATCCCGCCGGCCCTCGTCAAGCCGTCGGCTATGGAGGGTACCGGATTCCTCGGTCAAGCTG
CCGATGACGTTTACTACTTACCCAAGGATGACCAGTACCTTGTCGGAACTTCCGAGGTGGCTCTAGCAGCTTTCCATTCT
GACGAGATTCTCGACGATGCCGAGTTGCCCAAACGCTACGTCGCCTTTTCTCCATGCTACCGACGCGAGGCTGGCTCGTA
TGGCAAGGACACCCGCGGTATCTTTCGCGTTCATTGGTTCGACAAAGTCGAGATGTTCGTTTATTGCCTACCGCAGGAGG
CTGAGGCCTGGCATGAGAAGTTGCTGAGTTTCGAGAAGGACTTCATTACCGCGCTACAAATCCCGTTCCAGGTGCTCGAC
GTTGCCTCCGGTGACTTGGGCCTGTCTGCTGCCCGCAAGTTCGATTGTTATGGCTGGCTGCCAACCCAGAACCGCTATCG
TGAGATCACCTCGACGTCGAACTGCACTACCTTCCAGGCCCGGCGCCTGTCTATTCGCCATCGCGGACCTGACGGCGTTG
AACCCCTGGCCACTCTCAATGGCACCTTATGCGCGATGACTCGCATCATCATCATGCTGTTGGAGAACCACCAGCAGGCT
GATGGCTCGGTGCGCATACCCGAGGCACTGCGTCCTTATCTGGGTGGGCGTGAGTTCATTGAGCCTGTGAAGTGA

Upstream 100 bases:

>100_bases
AGCACACCAAGTGATGCACTGACGACGGGTGAGGCCCAGATTCCTACGGCCTGGGCCTCTGTCTGCGTCGGGATGCCATT
AGGCCGCTAGGATCGGTCAC

Downstream 100 bases:

>100_bases
ACGACGACCCGTACTACCGGGAGCTGCCCGACGGCACGATTAAGCAGGTTAACCCGTTTACTGGAACGAAAGTGTGGACG
GTTCCGGGCCGCGGTGCGCG

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MIDPKLLRTDPDAVRRSQAARGEDSSVVDDVVAADEARREAIAAHENLRAEQKGLGKRIAKASGDEKAELLSRTKTISGE
VADLKKAADEADAKFTELAKTLGNIVIDGVPAGGEDEGVIKETVGTPRDFAAEGFEPKDHLEIGEALGAIDMERGTKISG
SRFYVLTGVGAQLEFALLNLAMSKAAQWGFTPMIPPALVKPSAMEGTGFLGQAADDVYYLPKDDQYLVGTSEVALAAFHS
DEILDDAELPKRYVAFSPCYRREAGSYGKDTRGIFRVHWFDKVEMFVYCLPQEAEAWHEKLLSFEKDFITALQIPFQVLD
VASGDLGLSAARKFDCYGWLPTQNRYREITSTSNCTTFQARRLSIRHRGPDGVEPLATLNGTLCAMTRIIIMLLENHQQA
DGSVRIPEALRPYLGGREFIEPVK

Sequences:

>Translated_424_residues
MIDPKLLRTDPDAVRRSQAARGEDSSVVDDVVAADEARREAIAAHENLRAEQKGLGKRIAKASGDEKAELLSRTKTISGE
VADLKKAADEADAKFTELAKTLGNIVIDGVPAGGEDEGVIKETVGTPRDFAAEGFEPKDHLEIGEALGAIDMERGTKISG
SRFYVLTGVGAQLEFALLNLAMSKAAQWGFTPMIPPALVKPSAMEGTGFLGQAADDVYYLPKDDQYLVGTSEVALAAFHS
DEILDDAELPKRYVAFSPCYRREAGSYGKDTRGIFRVHWFDKVEMFVYCLPQEAEAWHEKLLSFEKDFITALQIPFQVLD
VASGDLGLSAARKFDCYGWLPTQNRYREITSTSNCTTFQARRLSIRHRGPDGVEPLATLNGTLCAMTRIIIMLLENHQQA
DGSVRIPEALRPYLGGREFIEPVK
>Mature_424_residues
MIDPKLLRTDPDAVRRSQAARGEDSSVVDDVVAADEARREAIAAHENLRAEQKGLGKRIAKASGDEKAELLSRTKTISGE
VADLKKAADEADAKFTELAKTLGNIVIDGVPAGGEDEGVIKETVGTPRDFAAEGFEPKDHLEIGEALGAIDMERGTKISG
SRFYVLTGVGAQLEFALLNLAMSKAAQWGFTPMIPPALVKPSAMEGTGFLGQAADDVYYLPKDDQYLVGTSEVALAAFHS
DEILDDAELPKRYVAFSPCYRREAGSYGKDTRGIFRVHWFDKVEMFVYCLPQEAEAWHEKLLSFEKDFITALQIPFQVLD
VASGDLGLSAARKFDCYGWLPTQNRYREITSTSNCTTFQARRLSIRHRGPDGVEPLATLNGTLCAMTRIIIMLLENHQQA
DGSVRIPEALRPYLGGREFIEPVK

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI8923421, Length=361, Percent_Identity=38.781163434903, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI16306548, Length=475, Percent_Identity=32, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI225579074, Length=352, Percent_Identity=39.2045454545455, Blast_Score=228, Evalue=1e-59,
Organism=Escherichia coli, GI1787120, Length=433, Percent_Identity=36.7205542725173, Blast_Score=266, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17539260, Length=478, Percent_Identity=31.1715481171548, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17542084, Length=392, Percent_Identity=33.6734693877551, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320226, Length=432, Percent_Identity=32.1759259259259, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6321799, Length=422, Percent_Identity=30.5687203791469, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24581334, Length=479, Percent_Identity=31.1064718162839, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI17737985, Length=285, Percent_Identity=36.4912280701754, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_PROAC (Q6A5P0)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056881.1
- HSSP:   Q9N0F3
- ProteinModelPortal:   Q6A5P0
- SMR:   Q6A5P0
- GeneID:   2931163
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA2219
- NMPDR:   fig|267747.1.peg.2158
- HOGENOM:   HBG629391
- OMA:   PKFDFKP
- ProtClustDB:   PRK05431
- BioCyc:   PACN267747:PPA2219-MONOMER
- BRENDA:   6.1.1.11
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 46532; Mature: 46532

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 277-277 BINDING 284-284 BINDING 382-382

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPKLLRTDPDAVRRSQAARGEDSSVVDDVVAADEARREAIAAHENLRAEQKGLGKRIA
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KASGDEKAELLSRTKTISGEVADLKKAADEADAKFTELAKTLGNIVIDGVPAGGEDEGVI
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHH
KETVGTPRDFAAEGFEPKDHLEIGEALGAIDMERGTKISGSRFYVLTGVGAQLEFALLNL
HHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHH
AMSKAAQWGFTPMIPPALVKPSAMEGTGFLGQAADDVYYLPKDDQYLVGTSEVALAAFHS
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCHHHEEEEECC
DEILDDAELPKRYVAFSPCYRREAGSYGKDTRGIFRVHWFDKVEMFVYCLPQEAEAWHEK
CHHCCCHHCCHHHHHCCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHH
LLSFEKDFITALQIPFQVLDVASGDLGLSAARKFDCYGWLPTQNRYREITSTSNCTTFQA
HHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEECCCCCHHHHHHCCCCCCCCHHH
RRLSIRHRGPDGVEPLATLNGTLCAMTRIIIMLLENHQQADGSVRIPEALRPYLGGREFI
HHHHEECCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHCCCHHHH
EPVK
CCCC
>Mature Secondary Structure
MIDPKLLRTDPDAVRRSQAARGEDSSVVDDVVAADEARREAIAAHENLRAEQKGLGKRIA
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KASGDEKAELLSRTKTISGEVADLKKAADEADAKFTELAKTLGNIVIDGVPAGGEDEGVI
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHH
KETVGTPRDFAAEGFEPKDHLEIGEALGAIDMERGTKISGSRFYVLTGVGAQLEFALLNL
HHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHH
AMSKAAQWGFTPMIPPALVKPSAMEGTGFLGQAADDVYYLPKDDQYLVGTSEVALAAFHS
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCHHHEEEEECC
DEILDDAELPKRYVAFSPCYRREAGSYGKDTRGIFRVHWFDKVEMFVYCLPQEAEAWHEK
CHHCCCHHCCHHHHHCCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHH
LLSFEKDFITALQIPFQVLDVASGDLGLSAARKFDCYGWLPTQNRYREITSTSNCTTFQA
HHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEECCCCCHHHHHHCCCCCCCCHHH
RRLSIRHRGPDGVEPLATLNGTLCAMTRIIIMLLENHQQADGSVRIPEALRPYLGGREFI
HHHHEECCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHCCCHHHH
EPVK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA