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The map label for this gene is lacI [H]

Identifier: 50843628

GI number: 50843628

Start: 2373463

End: 2374473

Strand: Reverse

Name: lacI [H]

Synonym: PPA2191

Alternate gene names: 50843628

Gene position: 2374473-2373463 (Counterclockwise)

Preceding gene: 50843629

Following gene: 50843625

Centisome position: 92.74

GC content: 63.4

Gene sequence:

>1011_bases
ATGACCGAGCGGCAGCGTCGAGGACCGTCTCGCCCCTCAATGGGTGATGTCGCCGCGGCTGCCGGGGTATCCCAACAGAC
CGTCTCGCGGGTCGTCAACAACTCCGACGCCGTTCGCAAGAAAACTGTCGACAAGGTTCTCAAGGCCATGACCGAAGTCG
GCTATTCGCCCAATGTGGCTGCTCGCAGCCTGCGCTCGGGCCGCACGAGTGCCATCGGCGTCCTTGCTACCGACCTTTCA
CGCACCGGAGAGCTGCGGACCGTTCAAGCCATTGTCGATGCCACCCGCCGCAGCGAGCTTGCCGTCGTCCTTGATCGTCT
TGAGCCTGCTGACGACTCCGTCAGGTCGTACCGGCACGCGATGAAGCGGATGGCGGGGCGAGTCGACGGCTTCATCATTG
AGGGGCTTGAGCTTGACCATCCCGAGGACCTTGAGGTACCGCCGGGAATCCCAGTCGTGATGGCTGGCTCTTCGTGCAGC
CGTTTCTCGACCGTCGGATGTGACCAGGCCTCCGGTGTGCGTACCGCCGTTAACTATCTCTTGGCATTGGGGCATCGCAC
AGTCCACCTCGTCAGCGGCCCGGACTATTCCCGCCAGGCTGTGGTACGCCGCCAGGCCTGGCAAGAGTGCTTGGAGACCA
ACGACCGCGAGATCCCCGATGTCATCATCGGGGACTGGACGGCCTCGTCAGGACGCGATGCGGCACGCTACCTCAAGGAC
GCCGGAGCAACAGCCGTGTTAGCCAGCAATGACGAAATGGCGGCAGGAGCCGTCATCGGTCTGCTCGATATGGGTGTCAG
GATCCCGGACGATATGTCTGTGGTGGGATTCGACGACGTCCTGGGAAATACGGTGTGGCCGACGATGACGACGGTGCGAC
AGGACTTCGCGGCGGTCGGTCAACGCCTCGCCGAGGAGCTCCTAGCCCAGTTGCAGGGTGCTCCGAGGAAGTCGGTCATG
GTTCCGGCCCCGCTGGTGATTCGCGAGAGTACCGCACCCCCGCGACATTGA

Upstream 100 bases:

>100_bases
GTTGCCACGCAGGTCGCGGACTCCTTCCGGGAGGCCGGTTTCGCCCACCCCGAGTTCTACGTCGTTACCCCTGGCCCTGC
TGCCCTGCGGGTGCAGTGAT

Downstream 100 bases:

>100_bases
CCCCGAGCGACGCCTACGAACCGCACAGGCGCGGCCCCAGGAGAGGCGTCAGGGCTGCTGCGGGTTATAGGCGCAGGCGT
CAGTCACAGATTGGGAGACG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTERQRRGPSRPSMGDVAAAAGVSQQTVSRVVNNSDAVRKKTVDKVLKAMTEVGYSPNVAARSLRSGRTSAIGVLATDLS
RTGELRTVQAIVDATRRSELAVVLDRLEPADDSVRSYRHAMKRMAGRVDGFIIEGLELDHPEDLEVPPGIPVVMAGSSCS
RFSTVGCDQASGVRTAVNYLLALGHRTVHLVSGPDYSRQAVVRRQAWQECLETNDREIPDVIIGDWTASSGRDAARYLKD
AGATAVLASNDEMAAGAVIGLLDMGVRIPDDMSVVGFDDVLGNTVWPTMTTVRQDFAAVGQRLAEELLAQLQGAPRKSVM
VPAPLVIRESTAPPRH

Sequences:

>Translated_336_residues
MTERQRRGPSRPSMGDVAAAAGVSQQTVSRVVNNSDAVRKKTVDKVLKAMTEVGYSPNVAARSLRSGRTSAIGVLATDLS
RTGELRTVQAIVDATRRSELAVVLDRLEPADDSVRSYRHAMKRMAGRVDGFIIEGLELDHPEDLEVPPGIPVVMAGSSCS
RFSTVGCDQASGVRTAVNYLLALGHRTVHLVSGPDYSRQAVVRRQAWQECLETNDREIPDVIIGDWTASSGRDAARYLKD
AGATAVLASNDEMAAGAVIGLLDMGVRIPDDMSVVGFDDVLGNTVWPTMTTVRQDFAAVGQRLAEELLAQLQGAPRKSVM
VPAPLVIRESTAPPRH
>Mature_335_residues
TERQRRGPSRPSMGDVAAAAGVSQQTVSRVVNNSDAVRKKTVDKVLKAMTEVGYSPNVAARSLRSGRTSAIGVLATDLSR
TGELRTVQAIVDATRRSELAVVLDRLEPADDSVRSYRHAMKRMAGRVDGFIIEGLELDHPEDLEVPPGIPVVMAGSSCSR
FSTVGCDQASGVRTAVNYLLALGHRTVHLVSGPDYSRQAVVRRQAWQECLETNDREIPDVIIGDWTASSGRDAARYLKDA
GATAVLASNDEMAAGAVIGLLDMGVRIPDDMSVVGFDDVLGNTVWPTMTTVRQDFAAVGQRLAEELLAQLQGAPRKSVMV
PAPLVIRESTAPPRH

Specific function: Repressor of the lactose operon. Binds allolactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=33.5347432024169, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=33.1325301204819, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=28.6567164179104, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=28.9156626506024, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=29.6969696969697, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790194, Length=302, Percent_Identity=29.8013245033113, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=27.4143302180685, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=28.3276450511945, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787580, Length=324, Percent_Identity=24.3827160493827, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=22.3270440251572, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786268, Length=323, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36095; Mature: 35964

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERQRRGPSRPSMGDVAAAAGVSQQTVSRVVNNSDAVRKKTVDKVLKAMTEVGYSPNVA
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHH
ARSLRSGRTSAIGVLATDLSRTGELRTVQAIVDATRRSELAVVLDRLEPADDSVRSYRHA
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MKRMAGRVDGFIIEGLELDHPEDLEVPPGIPVVMAGSSCSRFSTVGCDQASGVRTAVNYL
HHHHHHHHCCEEEECEECCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCCHHHHHHHHH
LALGHRTVHLVSGPDYSRQAVVRRQAWQECLETNDREIPDVIIGDWTASSGRDAARYLKD
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHH
AGATAVLASNDEMAAGAVIGLLDMGVRIPDDMSVVGFDDVLGNTVWPTMTTVRQDFAAVG
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHH
QRLAEELLAQLQGAPRKSVMVPAPLVIRESTAPPRH
HHHHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCC
>Mature Secondary Structure 
TERQRRGPSRPSMGDVAAAAGVSQQTVSRVVNNSDAVRKKTVDKVLKAMTEVGYSPNVA
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHH
ARSLRSGRTSAIGVLATDLSRTGELRTVQAIVDATRRSELAVVLDRLEPADDSVRSYRHA
HHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MKRMAGRVDGFIIEGLELDHPEDLEVPPGIPVVMAGSSCSRFSTVGCDQASGVRTAVNYL
HHHHHHHHCCEEEECEECCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCCHHHHHHHHH
LALGHRTVHLVSGPDYSRQAVVRRQAWQECLETNDREIPDVIIGDWTASSGRDAARYLKD
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHH
AGATAVLASNDEMAAGAVIGLLDMGVRIPDDMSVVGFDDVLGNTVWPTMTTVRQDFAAVG
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHH
QRLAEELLAQLQGAPRKSVMVPAPLVIRESTAPPRH
HHHHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 355891; 9278503; 1107032; 4571224; 4594037; 3286877; 7498473; 2178920; 8046748; 2040302; 1923807; 3064080; 2742823; 8683581; 10647179; 8638105 [H]