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The map label for this gene is pdhC [H]

Identifier: 50843531

GI number: 50843531

Start: 2266523

End: 2267932

Strand: Reverse

Name: pdhC [H]

Synonym: PPA2092

Alternate gene names: 50843531

Gene position: 2267932-2266523 (Counterclockwise)

Preceding gene: 50843532

Following gene: 50843530

Centisome position: 88.58

GC content: 64.61

Gene sequence:

>1410_bases
ATGAAGAACTACTGCATGCCCGACCCGGGCGAAGGACTTACTGAGGGAGAAGTTGTCTCCTGGCAGGTGTCTCCCGGCGA
CACCGTCAAGATCAACGATGTGCTGTGCGAGGTCGAGACCGCTAAATCGATCGTTGAGCTTCCCAGCCCGTTTGCCGGAA
CCGTGGCAAAACTGTGCGCTGAGCCTGGTGAGACGGTGGCCGTTGGAACGCCCTTGGTGACGATTGACGACGGTTCGGAG
GACGAGCCGGAGTTTCTCGTTGGTCACGTTACAGCGGAGCCCGGGCGTCGACGTCGTCGCCGCAGGGGTGCTGCGGTGTC
GACTGAGCGGGCGCGCGAGGAGGGTGCAGACACTCATCCTGAGCAATCGGTTTCGGAATCTCGGCACGACGCCGAGGCGA
AACCGGAGCAGCGTCTGACCGAGCCGGCTCCTCGTCAAGACCCACCGCGCATGGACCGCACCGCGCACATTCTTGCCAAG
CCGCCGGCCCGACGATTGGCAGCAGACCTCGGCGTCGACTTGTCTCAGGTGACCGGCACCGGTCCGCAGGGTGCAGTGAC
GAGGAGTGACGTCAAAGCGGCCGCTCATCATGGCGCTGCTGCAGCCGGGGACGGCGGTGCTTGTCCTGGCGACGCCGAGC
TGGCGAGCCTGTCGGTGATGTCTCGACGTCTCCTCGGTGGCGCCCCGACCGAGCCCGACGGCCACACCCGCAAGGTGCCA
GTGCGTGGGGTGCGCAAGGTCACAGCGAAGGCCGTGAAGGATTCCTTGGATACGAAGGCCCTCGTGACGGCCTTCCTTAC
CTGCGACGTCACCCCGACGATGGAGCTGGTCAACCGGCTGCGTGCCGACCGTCGTTTCAAAGGCCTGCGTGTCTCCCCGC
TGACGGTGTGGTGCAAGGCGGTGTGCCTGGCGATGGGGCGCACCCCCATCATCAACGCCGCATGGGATGACGCCGCTGAC
CAGATCGTCTTCCGTGACCACATCAACCTCGGCATTGCCGCCGCCACGCCTCGCGGCCTTATGGTGCCGGTGGTGCGCGA
CGCCCAGGACATGGCGATGCTTGAGTTGGCGACGGAGATCACCCGGATCGTTGCGATCGCCAAGGAAGACAAACTGCAAC
CGCCCGACTATGCCGACGGTACCTTCTCGATCACGAACGTCGGGGTGTTCGGGCTCGATGCCGGTACTCCCGTGGTCAAC
CGGACAGAGTCAGCGATCCTCGTGCTCGGTGCTCTCGCTCGGCGGCCGTGGGTCGTCGGGACCGGTGATGACGAGCGCGT
TGTGCCCAGGTGGGTGACGACGATGAGCCTCGGGTTCGATCATCGTCTTATTGACGGTGAGCAGGGTTCGACATTCCTCC
ACGACGTTGCGGAGATCCTCTCTGACCCGGCGTCGGCAATGCTGTACTGA

Upstream 100 bases:

>100_bases
ACCCGCCAGCCAAGGCTGAGGGGGAACACATCCCGGACGTCGACCGCATTCTCGACGCTGTAGACCGCTCTCTGGCCTTC
TGATTCTGAGAGGCATTCAC

Downstream 100 bases:

>100_bases
CCCGATTCTGAGAAGAATTGCGGGAGCGCGATATGTGCCACCGAATTTGCCACAAGATTGACACTGCCTCACTTTAAGTG
AAAGTATGGATGCGGTAGCG

Product: dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKNYCMPDPGEGLTEGEVVSWQVSPGDTVKINDVLCEVETAKSIVELPSPFAGTVAKLCAEPGETVAVGTPLVTIDDGSE
DEPEFLVGHVTAEPGRRRRRRRGAAVSTERAREEGADTHPEQSVSESRHDAEAKPEQRLTEPAPRQDPPRMDRTAHILAK
PPARRLAADLGVDLSQVTGTGPQGAVTRSDVKAAAHHGAAAAGDGGACPGDAELASLSVMSRRLLGGAPTEPDGHTRKVP
VRGVRKVTAKAVKDSLDTKALVTAFLTCDVTPTMELVNRLRADRRFKGLRVSPLTVWCKAVCLAMGRTPIINAAWDDAAD
QIVFRDHINLGIAAATPRGLMVPVVRDAQDMAMLELATEITRIVAIAKEDKLQPPDYADGTFSITNVGVFGLDAGTPVVN
RTESAILVLGALARRPWVVGTGDDERVVPRWVTTMSLGFDHRLIDGEQGSTFLHDVAEILSDPASAMLY

Sequences:

>Translated_469_residues
MKNYCMPDPGEGLTEGEVVSWQVSPGDTVKINDVLCEVETAKSIVELPSPFAGTVAKLCAEPGETVAVGTPLVTIDDGSE
DEPEFLVGHVTAEPGRRRRRRRGAAVSTERAREEGADTHPEQSVSESRHDAEAKPEQRLTEPAPRQDPPRMDRTAHILAK
PPARRLAADLGVDLSQVTGTGPQGAVTRSDVKAAAHHGAAAAGDGGACPGDAELASLSVMSRRLLGGAPTEPDGHTRKVP
VRGVRKVTAKAVKDSLDTKALVTAFLTCDVTPTMELVNRLRADRRFKGLRVSPLTVWCKAVCLAMGRTPIINAAWDDAAD
QIVFRDHINLGIAAATPRGLMVPVVRDAQDMAMLELATEITRIVAIAKEDKLQPPDYADGTFSITNVGVFGLDAGTPVVN
RTESAILVLGALARRPWVVGTGDDERVVPRWVTTMSLGFDHRLIDGEQGSTFLHDVAEILSDPASAMLY
>Mature_469_residues
MKNYCMPDPGEGLTEGEVVSWQVSPGDTVKINDVLCEVETAKSIVELPSPFAGTVAKLCAEPGETVAVGTPLVTIDDGSE
DEPEFLVGHVTAEPGRRRRRRRGAAVSTERAREEGADTHPEQSVSESRHDAEAKPEQRLTEPAPRQDPPRMDRTAHILAK
PPARRLAADLGVDLSQVTGTGPQGAVTRSDVKAAAHHGAAAAGDGGACPGDAELASLSVMSRRLLGGAPTEPDGHTRKVP
VRGVRKVTAKAVKDSLDTKALVTAFLTCDVTPTMELVNRLRADRRFKGLRVSPLTVWCKAVCLAMGRTPIINAAWDDAAD
QIVFRDHINLGIAAATPRGLMVPVVRDAQDMAMLELATEITRIVAIAKEDKLQPPDYADGTFSITNVGVFGLDAGTPVVN
RTESAILVLGALARRPWVVGTGDDERVVPRWVTTMSLGFDHRLIDGEQGSTFLHDVAEILSDPASAMLY

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=476, Percent_Identity=26.4705882352941, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=34.070796460177, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI31711992, Length=461, Percent_Identity=25.3796095444685, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI203098753, Length=478, Percent_Identity=26.1506276150628, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI203098816, Length=480, Percent_Identity=26.0416666666667, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=32.2981366459627, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786946, Length=467, Percent_Identity=29.3361884368308, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1786305, Length=320, Percent_Identity=29.6875, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17537937, Length=480, Percent_Identity=26.0416666666667, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=474, Percent_Identity=28.0590717299578, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=234, Percent_Identity=32.0512820512821, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=26.1146496815287, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=240, Percent_Identity=36.25, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=473, Percent_Identity=22.8329809725159, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI18859875, Length=323, Percent_Identity=30.030959752322, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645909, Length=245, Percent_Identity=31.4285714285714, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=28.1385281385281, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=28.1385281385281, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 50076; Mature: 50076

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYCMPDPGEGLTEGEVVSWQVSPGDTVKINDVLCEVETAKSIVELPSPFAGTVAKLCA
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEEHHHEEEHHHHHHHHHCCCCHHHHHHHHHC
EPGETVAVGTPLVTIDDGSEDEPEFLVGHVTAEPGRRRRRRRGAAVSTERAREEGADTHP
CCCCEEEECCCEEEECCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCHHHHHHCCCCCCC
EQSVSESRHDAEAKPEQRLTEPAPRQDPPRMDRTAHILAKPPARRLAADLGVDLSQVTGT
HHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHCCC
GPQGAVTRSDVKAAAHHGAAAAGDGGACPGDAELASLSVMSRRLLGGAPTEPDGHTRKVP
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECC
VRGVRKVTAKAVKDSLDTKALVTAFLTCDVTPTMELVNRLRADRRFKGLRVSPLTVWCKA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VCLAMGRTPIINAAWDDAADQIVFRDHINLGIAAATPRGLMVPVVRDAQDMAMLELATEI
HHHHHCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHH
TRIVAIAKEDKLQPPDYADGTFSITNVGVFGLDAGTPVVNRTESAILVLGALARRPWVVG
HHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEE
TGDDERVVPRWVTTMSLGFDHRLIDGEQGSTFLHDVAEILSDPASAMLY
CCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MKNYCMPDPGEGLTEGEVVSWQVSPGDTVKINDVLCEVETAKSIVELPSPFAGTVAKLCA
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEEHHHEEEHHHHHHHHHCCCCHHHHHHHHHC
EPGETVAVGTPLVTIDDGSEDEPEFLVGHVTAEPGRRRRRRRGAAVSTERAREEGADTHP
CCCCEEEECCCEEEECCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCHHHHHHCCCCCCC
EQSVSESRHDAEAKPEQRLTEPAPRQDPPRMDRTAHILAKPPARRLAADLGVDLSQVTGT
HHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHCCC
GPQGAVTRSDVKAAAHHGAAAAGDGGACPGDAELASLSVMSRRLLGGAPTEPDGHTRKVP
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECC
VRGVRKVTAKAVKDSLDTKALVTAFLTCDVTPTMELVNRLRADRRFKGLRVSPLTVWCKA
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VCLAMGRTPIINAAWDDAADQIVFRDHINLGIAAATPRGLMVPVVRDAQDMAMLELATEI
HHHHHCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHH
TRIVAIAKEDKLQPPDYADGTFSITNVGVFGLDAGTPVVNRTESAILVLGALARRPWVVG
HHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEE
TGDDERVVPRWVTTMSLGFDHRLIDGEQGSTFLHDVAEILSDPASAMLY
CCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA