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The map label for this gene is trmH [C]

Identifier: 50843459

GI number: 50843459

Start: 2185822

End: 2186412

Strand: Reverse

Name: trmH [C]

Synonym: PPA2013

Alternate gene names: 50843459

Gene position: 2186412-2185822 (Counterclockwise)

Preceding gene: 50843461

Following gene: 50843458

Centisome position: 85.4

GC content: 58.38

Gene sequence:

>591_bases
ATGCCCAAACCGTGGCCTGATGACCCGAGACTCGATCCTGAGCTGTTGGCCTCAGGCGATCGACGCAATGTTATTGATCG
GTACCGCTACTGGACGATGGAGGCCATCGTCGCCGACCTTGACGCCCAGCGCTCCCGGTTGCATGTGGCTATTCAGAACT
GGGAACACGATTTCAATATCGGGTCGGTGGTGCGTACCGCCAACGCTTTTAATGTCGCGGCCGTTCATATCTTGGGGCGG
CGTCGTTGGAATAGACGTGGCGCAATGGTGACTGACCGGTATTTGCACGTTTACCATCACTCTGACGTCCCCTCCTTCCT
ACACTGGCTTGACGAGCACGGCGTCACTCCCGTCGGAGTGGATAATCTGCCTGGATCGGTGCCACTGGAGACGGTCCAGC
TGCCGCAGGACTGTTGCTTGGTCTTTGGATCGGAAGGTCCCGGGTTGACCGACGAGGTCGTCAAAGGATGCGAGCAGTTG
GTGGCTATCACCCAGTACGGGTCAACGCGTTCCATGAATGCTGGTGCGGCTGCCGCGATTGCTATGTACGCCTGGACGAC
GCAGTGGTGTCCAAAGCCACGCACTAGCTGA

Upstream 100 bases:

>100_bases
TCTTTGGTCCGTGTGTGCCTCGTCCGCTGATGAACTCGCTGATGCCCCTTCACTGCAGACTCCCAGCCAGTTAGGGACTA
CAGTCGTAGGTGTTGGGCCG

Downstream 100 bases:

>100_bases
TCGGGGAGAACCGTCACCCTCTAGGCTCGTGTCATGAGCACGCAACCCACCGAGGAACCACTCCGACTAGTTGTGGCATT
CAATCCAGTGCCTAGTGCCT

Product: rRNA methylase (SpoU family protein)

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: RNA Methyltransferase; rRNA Methylase; TRNA/RRNA MethyltransferaU; RNA Methyltransferase TrmH Family; 23S RRNA Methyltransferase; TrmH Family RNA Methyltransferase; RNA Methylase Family Protein; Spou RRNA Methylase Family Protein; Methyltransferase; SPOU RRNA Methylase Family Protein

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MPKPWPDDPRLDPELLASGDRRNVIDRYRYWTMEAIVADLDAQRSRLHVAIQNWEHDFNIGSVVRTANAFNVAAVHILGR
RRWNRRGAMVTDRYLHVYHHSDVPSFLHWLDEHGVTPVGVDNLPGSVPLETVQLPQDCCLVFGSEGPGLTDEVVKGCEQL
VAITQYGSTRSMNAGAAAAIAMYAWTTQWCPKPRTS

Sequences:

>Translated_196_residues
MPKPWPDDPRLDPELLASGDRRNVIDRYRYWTMEAIVADLDAQRSRLHVAIQNWEHDFNIGSVVRTANAFNVAAVHILGR
RRWNRRGAMVTDRYLHVYHHSDVPSFLHWLDEHGVTPVGVDNLPGSVPLETVQLPQDCCLVFGSEGPGLTDEVVKGCEQL
VAITQYGSTRSMNAGAAAAIAMYAWTTQWCPKPRTS
>Mature_195_residues
PKPWPDDPRLDPELLASGDRRNVIDRYRYWTMEAIVADLDAQRSRLHVAIQNWEHDFNIGSVVRTANAFNVAAVHILGRR
RWNRRGAMVTDRYLHVYHHSDVPSFLHWLDEHGVTPVGVDNLPGSVPLETVQLPQDCCLVFGSEGPGLTDEVVKGCEQLV
AITQYGSTRSMNAGAAAAIAMYAWTTQWCPKPRTS

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 22032; Mature: 21901

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPWPDDPRLDPELLASGDRRNVIDRYRYWTMEAIVADLDAQRSRLHVAIQNWEHDFNI
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCH
GSVVRTANAFNVAAVHILGRRRWNRRGAMVTDRYLHVYHHSDVPSFLHWLDEHGVTPVGV
HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEHHEEEEEECCCHHHHHHHHHHCCCCCCCC
DNLPGSVPLETVQLPQDCCLVFGSEGPGLTDEVVKGCEQLVAITQYGSTRSMNAGAAAAI
CCCCCCCCCHHHCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AMYAWTTQWCPKPRTS
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PKPWPDDPRLDPELLASGDRRNVIDRYRYWTMEAIVADLDAQRSRLHVAIQNWEHDFNI
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCH
GSVVRTANAFNVAAVHILGRRRWNRRGAMVTDRYLHVYHHSDVPSFLHWLDEHGVTPVGV
HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEHHEEEEEECCCHHHHHHHHHHCCCCCCCC
DNLPGSVPLETVQLPQDCCLVFGSEGPGLTDEVVKGCEQLVAITQYGSTRSMNAGAAAAI
CCCCCCCCCHHHCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AMYAWTTQWCPKPRTS
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA