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The map label for this gene is nanT1 [H]

Identifier: 50843447

GI number: 50843447

Start: 2172454

End: 2174058

Strand: Reverse

Name: nanT1 [H]

Synonym: PPA2000

Alternate gene names: 50843447

Gene position: 2174058-2172454 (Counterclockwise)

Preceding gene: 50843448

Following gene: 50843446

Centisome position: 84.92

GC content: 58.26

Gene sequence:

>1605_bases
ATGAGCAACGTCGAGGCCATAGCAGCCAGGAAATCATGGCGTGACGAGTTGACTGGCACGCAGTGGAAATCCTTCTGGGC
AGCATGGATCGGTTATCTGCTGGACGGATTCGACTTCGTCATGATCACGCTGGTCCTCACCGAGATCGGCAGGACCTTTC
ACGTCGGACCAGCGGCGACTGCGACCCTGGTGTCCGCTGCTTTTATTGCGCGATGGTTCGGCGGACTGCTGCTGGGAGCT
ATCGGTGACCGGATCGGCCGCAAGGAGGCTATGGTCATCTCAATTCTGCTTTATGCGGGTGGATCGGTCGTCTGTGCCAT
TGCCCCGGCTTTCTGGGTTATCTTCGTCGCCCGTGTCTGCATCGGCATGGGTATGGCCGGTGAGTACGGCTCTTCAGCCA
CCTACGTCATTGAGTCCTGGCCAGCGCGTCTGCGGAATAAGGCCTCCGGGTTCCTTATTTCCGGATTTTCCCTTGGCGCC
GGTGTGACGGCTCAGGTGTATGCCCTCATCGAGTACCTTACCCGTGGTACCTCCGCTGAGCCTCATACCTGGCGAATACT
CTTCGCGCTTGGCATTATTCCGATCGCCTTGGCCCTGTGGCTGCGTCGTGCCCTTCCTGAGGCCGCCGACTTTGTAAAAA
TGCGCGAGGAGGAGGCCGCTCGCGCCGCTGTCGGCGAGACTGTCAAGAAGACTGACATGTTCACCATGCTCTTCGCACGT
AACCCGAAGCGAAGCATCATTAACATTGTTCTGGCCCTCACAACTTTCGGATGCCTAATCGTCATTTACCTGTTCAAGAG
CGGCGTCCCGGGGCTCGTGTTGGCATTGCTGTGGGTCGTTGTCGCGGCCGTCATGGTGAGCTTCATGGTCCAGTTCGAGG
GACCGCGTTGGCCGACAGGTGTGGCCGTTATGATCACGGTGTTTGTCGCCTTCCTCTACTCCTGGCCTTTGCAGGCTCTG
TTGCCTACCTACTATCGCCAAACCCTGGGCCTGGCGAGCGACACCGCTTCCAATCTCGTGACCGCCACTTCTTTTGGCGC
GGCAGCGGGCTGCATCATGGCCGGATTTATCGGTGACAGATTCGGAACCCGCAAGGCCTATTGGGTCTCCCTGCTCATCT
CGGAGTTCCTCGTCCTGCCGGTCTTCCTCGTCAGCCGAGGCATGGTGCATTCCTCGGTGATATGGGTCGTGCTTCTTGGA
GTTTTCATCTTTGTCCAGCAGATGTTCGGACAAGGTATCTCTGGTCTGCTACCGAAGTTCATTTCGAACTACTTTCCGGT
CGAGAAGCGCGCTGCTGGTTTGGGTTTCTCTTATAACGTCGGAGCTCTTGGTGGGGCTATCGCCCCGGTCCTCGGCATTG
CCCTGGCAGGTGATCCAGCTACCGGTGTTGGCGGTGTGCTGCCCTCCAGCTGGGGCCTCGGATGGACCCTGTTCATCTTG
TCCTTCGTATTCACCGCGGTGATCATCGTTCTTATCGCCATCAATTTCCCGCTCCGGATGCAGAAGCTGTTGCGCCCCGA
CCACGTGCGTCCCGCTGACCGGATGGATCACGTCGATGAAGAGCTTGCTGTTTCCCGGAACCCTGACACTATCGTCGCCT
CGTGA

Upstream 100 bases:

>100_bases
AGGTTGACAACATGATTCTGGTGATTCATCCTTGAGATAGGACATCCAAGGTCCAATGTAATGCGACGGGTTAACCCGAC
CAATCAATGGAGATGGGCGT

Downstream 100 bases:

>100_bases
TGCGAGCGAGAAGGAGGACGTATCATGACTGCTCCGACCATCGTTGGCGCATACGCCGCGATGCCACGGGCCATCGCTGA
GCGGGAGTGCTTGTACCGGA

Product: putative sialic acid transporter

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: Sialic acid permease 1 [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSNVEAIAARKSWRDELTGTQWKSFWAAWIGYLLDGFDFVMITLVLTEIGRTFHVGPAATATLVSAAFIARWFGGLLLGA
IGDRIGRKEAMVISILLYAGGSVVCAIAPAFWVIFVARVCIGMGMAGEYGSSATYVIESWPARLRNKASGFLISGFSLGA
GVTAQVYALIEYLTRGTSAEPHTWRILFALGIIPIALALWLRRALPEAADFVKMREEEAARAAVGETVKKTDMFTMLFAR
NPKRSIINIVLALTTFGCLIVIYLFKSGVPGLVLALLWVVVAAVMVSFMVQFEGPRWPTGVAVMITVFVAFLYSWPLQAL
LPTYYRQTLGLASDTASNLVTATSFGAAAGCIMAGFIGDRFGTRKAYWVSLLISEFLVLPVFLVSRGMVHSSVIWVVLLG
VFIFVQQMFGQGISGLLPKFISNYFPVEKRAAGLGFSYNVGALGGAIAPVLGIALAGDPATGVGGVLPSSWGLGWTLFIL
SFVFTAVIIVLIAINFPLRMQKLLRPDHVRPADRMDHVDEELAVSRNPDTIVAS

Sequences:

>Translated_534_residues
MSNVEAIAARKSWRDELTGTQWKSFWAAWIGYLLDGFDFVMITLVLTEIGRTFHVGPAATATLVSAAFIARWFGGLLLGA
IGDRIGRKEAMVISILLYAGGSVVCAIAPAFWVIFVARVCIGMGMAGEYGSSATYVIESWPARLRNKASGFLISGFSLGA
GVTAQVYALIEYLTRGTSAEPHTWRILFALGIIPIALALWLRRALPEAADFVKMREEEAARAAVGETVKKTDMFTMLFAR
NPKRSIINIVLALTTFGCLIVIYLFKSGVPGLVLALLWVVVAAVMVSFMVQFEGPRWPTGVAVMITVFVAFLYSWPLQAL
LPTYYRQTLGLASDTASNLVTATSFGAAAGCIMAGFIGDRFGTRKAYWVSLLISEFLVLPVFLVSRGMVHSSVIWVVLLG
VFIFVQQMFGQGISGLLPKFISNYFPVEKRAAGLGFSYNVGALGGAIAPVLGIALAGDPATGVGGVLPSSWGLGWTLFIL
SFVFTAVIIVLIAINFPLRMQKLLRPDHVRPADRMDHVDEELAVSRNPDTIVAS
>Mature_533_residues
SNVEAIAARKSWRDELTGTQWKSFWAAWIGYLLDGFDFVMITLVLTEIGRTFHVGPAATATLVSAAFIARWFGGLLLGAI
GDRIGRKEAMVISILLYAGGSVVCAIAPAFWVIFVARVCIGMGMAGEYGSSATYVIESWPARLRNKASGFLISGFSLGAG
VTAQVYALIEYLTRGTSAEPHTWRILFALGIIPIALALWLRRALPEAADFVKMREEEAARAAVGETVKKTDMFTMLFARN
PKRSIINIVLALTTFGCLIVIYLFKSGVPGLVLALLWVVVAAVMVSFMVQFEGPRWPTGVAVMITVFVAFLYSWPLQALL
PTYYRQTLGLASDTASNLVTATSFGAAAGCIMAGFIGDRFGTRKAYWVSLLISEFLVLPVFLVSRGMVHSSVIWVVLLGV
FIFVQQMFGQGISGLLPKFISNYFPVEKRAAGLGFSYNVGALGGAIAPVLGIALAGDPATGVGGVLPSSWGLGWTLFILS
FVFTAVIIVLIAINFPLRMQKLLRPDHVRPADRMDHVDEELAVSRNPDTIVAS

Specific function: May be a sugar-cation symporter involved in sialic acid uptake [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Sialic acid transporter (TC 2.A.1.12.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082231, Length=505, Percent_Identity=49.5049504950495, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI87082404, Length=493, Percent_Identity=32.0486815415822, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1790550, Length=456, Percent_Identity=21.9298245614035, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788292, Length=210, Percent_Identity=29.5238095238095, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788942, Length=207, Percent_Identity=29.4685990338164, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004742
- InterPro:   IPR005828 [H]

Pfam domain/function: PF07690 MFS_1; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 57764; Mature: 57633

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVEAIAARKSWRDELTGTQWKSFWAAWIGYLLDGFDFVMITLVLTEIGRTFHVGPAAT
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHH
ATLVSAAFIARWFGGLLLGAIGDRIGRKEAMVISILLYAGGSVVCAIAPAFWVIFVARVC
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGMGMAGEYGSSATYVIESWPARLRNKASGFLISGFSLGAGVTAQVYALIEYLTRGTSAE
HHCCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
PHTWRILFALGIIPIALALWLRRALPEAADFVKMREEEAARAAVGETVKKTDMFTMLFAR
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPKRSIINIVLALTTFGCLIVIYLFKSGVPGLVLALLWVVVAAVMVSFMVQFEGPRWPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
VAVMITVFVAFLYSWPLQALLPTYYRQTLGLASDTASNLVTATSFGAAAGCIMAGFIGDR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGTRKAYWVSLLISEFLVLPVFLVSRGMVHSSVIWVVLLGVFIFVQQMFGQGISGLLPKF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ISNYFPVEKRAAGLGFSYNVGALGGAIAPVLGIALAGDPATGVGGVLPSSWGLGWTLFIL
HHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
SFVFTAVIIVLIAINFPLRMQKLLRPDHVRPADRMDHVDEELAVSRNPDTIVAS
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure 
SNVEAIAARKSWRDELTGTQWKSFWAAWIGYLLDGFDFVMITLVLTEIGRTFHVGPAAT
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHH
ATLVSAAFIARWFGGLLLGAIGDRIGRKEAMVISILLYAGGSVVCAIAPAFWVIFVARVC
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGMGMAGEYGSSATYVIESWPARLRNKASGFLISGFSLGAGVTAQVYALIEYLTRGTSAE
HHCCCCCCCCCCCEEEHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
PHTWRILFALGIIPIALALWLRRALPEAADFVKMREEEAARAAVGETVKKTDMFTMLFAR
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPKRSIINIVLALTTFGCLIVIYLFKSGVPGLVLALLWVVVAAVMVSFMVQFEGPRWPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
VAVMITVFVAFLYSWPLQALLPTYYRQTLGLASDTASNLVTATSFGAAAGCIMAGFIGDR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGTRKAYWVSLLISEFLVLPVFLVSRGMVHSSVIWVVLLGVFIFVQQMFGQGISGLLPKF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ISNYFPVEKRAAGLGFSYNVGALGGAIAPVLGIALAGDPATGVGGVLPSSWGLGWTLFIL
HHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
SFVFTAVIIVLIAINFPLRMQKLLRPDHVRPADRMDHVDEELAVSRNPDTIVAS
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]