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The map label for this gene is dhaK [H]

Identifier: 50843396

GI number: 50843396

Start: 2108896

End: 2109924

Strand: Direct

Name: dhaK [H]

Synonym: PPA1947

Alternate gene names: 50843396

Gene position: 2108896-2109924 (Clockwise)

Preceding gene: 50843395

Following gene: 50843397

Centisome position: 82.37

GC content: 60.45

Gene sequence:

>1029_bases
ATGGGGTCAAGATGGTTCACTAGTGCCATGAAGAAACTCATCAACGCTGTCGAGGATGTCGTCGTTGATTCCCTGCGCGG
CGTAGCGGCCGCTCACCCAGACTGTCTGAAAGTCGATCTCGATCATCGCATAGTGTTGCGCGCCAGTGGTCCGGTGCACG
GAAAGGTTGGTCTGGTCTCTGGTGGCGGTTCCGGACACGAGCCGCTGCACTCGGGCTACGTCGGACGAGGAATGCTCGAC
GCCGCATGCTGCGGCGAGGTCTTCACCTCCCCCGTCCCCGACCAGATCACCGAGGCCACCAAGGCCGTCGATGGTGGGGT
TGGCGTACTCCACATCGTTAAGAACTACACCGGTGACGTCATGAACTTCGATATGGCTGCCGAGATGGCTTCGATGGAGG
CTGGCACCCGCGTCGAGTCCGTCGTGATCGCTGACGATGTTGCCGTACAGGACTCCCTGTACACCGCTGGGCGTCGCGGG
GTCGGTTTGACGGTGCTGGCCGAAAAGATCCTCGGCGCAGCAGCCGAGGACAAGCGCGATCTCGACACCCTGCTCGAGCT
TACTAAGCGCATTAATGACGGTGGCCGCTCCTTTGGTGTGGCCCTTACCTCCTGCACTGTCCCGGCAGCCGGCAAGCCCA
CTTTCGACCTTGACGAGAACACGATGGAGTTGGGCGTCGGCATCCACGGTGAGCCTGGTCGCGAAACCACCACGTTGGGC
ACTGCCTCCGAGGTCGCTGCCCAGATCGTCGAACCCATCCTGGCTGACCGTGACTTCACCACAACCCCGGTCATTGCCAT
GCTTAACGGCATGGGCGGCACCCCTCTCATCGAGCAGTATCTCGTGTGGGGTGAAATCGCCAAAATTTTGGCAGGAAAAG
GCGTCAAGATTGCCCGCACCCTGGTCGGCAATCACATTACGAGCCTAGAGATGGCAGGATGTTCCTTGACCTTACTCTCC
GCTGACGACGATCTCCTGACGCTGTGGGATGCCCCGGTGGAGACTCCTGGACTTCGATGGGGATGCTGA

Upstream 100 bases:

>100_bases
GGCAAGGTAACTATCGACTACGTCCAGCAGACGAGGTGAGTTAGCCAGTTGTGCAACAGCAGGTGAGTGCGTTAATCCAT
CTGATCGAGCCCCTGGAAAA

Downstream 100 bases:

>100_bases
TGTCTGAAATTTCTGCCGCAACCGTTTCCGAGTGGCTCAAGGACTACTCGGCGATCATCTCGGAACACGCGAATGAGCTC
ACTGACCTGGATCGTCAGAT

Product: hypothetical protein

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MGSRWFTSAMKKLINAVEDVVVDSLRGVAAAHPDCLKVDLDHRIVLRASGPVHGKVGLVSGGGSGHEPLHSGYVGRGMLD
AACCGEVFTSPVPDQITEATKAVDGGVGVLHIVKNYTGDVMNFDMAAEMASMEAGTRVESVVIADDVAVQDSLYTAGRRG
VGLTVLAEKILGAAAEDKRDLDTLLELTKRINDGGRSFGVALTSCTVPAAGKPTFDLDENTMELGVGIHGEPGRETTTLG
TASEVAAQIVEPILADRDFTTTPVIAMLNGMGGTPLIEQYLVWGEIAKILAGKGVKIARTLVGNHITSLEMAGCSLTLLS
ADDDLLTLWDAPVETPGLRWGC

Sequences:

>Translated_342_residues
MGSRWFTSAMKKLINAVEDVVVDSLRGVAAAHPDCLKVDLDHRIVLRASGPVHGKVGLVSGGGSGHEPLHSGYVGRGMLD
AACCGEVFTSPVPDQITEATKAVDGGVGVLHIVKNYTGDVMNFDMAAEMASMEAGTRVESVVIADDVAVQDSLYTAGRRG
VGLTVLAEKILGAAAEDKRDLDTLLELTKRINDGGRSFGVALTSCTVPAAGKPTFDLDENTMELGVGIHGEPGRETTTLG
TASEVAAQIVEPILADRDFTTTPVIAMLNGMGGTPLIEQYLVWGEIAKILAGKGVKIARTLVGNHITSLEMAGCSLTLLS
ADDDLLTLWDAPVETPGLRWGC
>Mature_341_residues
GSRWFTSAMKKLINAVEDVVVDSLRGVAAAHPDCLKVDLDHRIVLRASGPVHGKVGLVSGGGSGHEPLHSGYVGRGMLDA
ACCGEVFTSPVPDQITEATKAVDGGVGVLHIVKNYTGDVMNFDMAAEMASMEAGTRVESVVIADDVAVQDSLYTAGRRGV
GLTVLAEKILGAAAEDKRDLDTLLELTKRINDGGRSFGVALTSCTVPAAGKPTFDLDENTMELGVGIHGEPGRETTTLGT
ASEVAAQIVEPILADRDFTTTPVIAMLNGMGGTPLIEQYLVWGEIAKILAGKGVKIARTLVGNHITSLEMAGCSLTLLSA
DDDLLTLWDAPVETPGLRWGC

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=342, Percent_Identity=43.2748538011696, Blast_Score=228, Evalue=6e-60,
Organism=Escherichia coli, GI87081857, Length=357, Percent_Identity=49.859943977591, Blast_Score=334, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17565018, Length=331, Percent_Identity=35.6495468277946, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321055, Length=337, Percent_Identity=40.0593471810089, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323570, Length=324, Percent_Identity=37.037037037037, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 35778; Mature: 35647

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSRWFTSAMKKLINAVEDVVVDSLRGVAAAHPDCLKVDLDHRIVLRASGPVHGKVGLVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCEEEEEE
GGGSGHEPLHSGYVGRGMLDAACCGEVFTSPVPDQITEATKAVDGGVGVLHIVKNYTGDV
CCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCC
MNFDMAAEMASMEAGTRVESVVIADDVAVQDSLYTAGRRGVGLTVLAEKILGAAAEDKRD
EEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHH
LDTLLELTKRINDGGRSFGVALTSCTVPAAGKPTFDLDENTMELGVGIHGEPGRETTTLG
HHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
TASEVAAQIVEPILADRDFTTTPVIAMLNGMGGTPLIEQYLVWGEIAKILAGKGVKIART
CHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVGNHITSLEMAGCSLTLLSADDDLLTLWDAPVETPGLRWGC
HHHCCCCEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
GSRWFTSAMKKLINAVEDVVVDSLRGVAAAHPDCLKVDLDHRIVLRASGPVHGKVGLVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCEEEEEE
GGGSGHEPLHSGYVGRGMLDAACCGEVFTSPVPDQITEATKAVDGGVGVLHIVKNYTGDV
CCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCC
MNFDMAAEMASMEAGTRVESVVIADDVAVQDSLYTAGRRGVGLTVLAEKILGAAAEDKRD
EEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHH
LDTLLELTKRINDGGRSFGVALTSCTVPAAGKPTFDLDENTMELGVGIHGEPGRETTTLG
HHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
TASEVAAQIVEPILADRDFTTTPVIAMLNGMGGTPLIEQYLVWGEIAKILAGKGVKIART
CHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVGNHITSLEMAGCSLTLLSADDDLLTLWDAPVETPGLRWGC
HHHCCCCEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]