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The map label for this gene is nikA [C]

Identifier: 50843296

GI number: 50843296

Start: 2004533

End: 2006236

Strand: Reverse

Name: nikA [C]

Synonym: PPA1840

Alternate gene names: 50843296

Gene position: 2006236-2004533 (Counterclockwise)

Preceding gene: 50843297

Following gene: 50843295

Centisome position: 78.36

GC content: 58.1

Gene sequence:

>1704_bases
TTGCGCAAGGCAATCGTGACCCCGGTGGCAGTGCTGGCCGTGTTGGTTATGGCACTCACCGGGTGCGGACAGAAGAACCA
GTCTGGCGGAAGTAAGCAGTCGGGGGTGACATCGGAGTCGGCCCCGATGGCTCAACTCAACGTGGTTGACCGCGGTCAAC
TCAAGGACGGCGGAACGCTGCGTCTGGCCATCGAGCAGCTGCCGACGGAGTGGAACCCCCTGAACGTCAACGGCGACACC
GCTGACTTGGGCACAACGATATGGCGGTTCGTGGGTGCCACCAACTTCGATATCACCGAAGACGGCACCCCGAAGCCGAA
CCCGAATTACATCTTCTCGACGGACGTGGAGACCAAGGGTGGTAAGCAGGTCGTGACCTTGCACCTCAACCCGAAGGCTA
AGTGGAACTCGGGTCGCACCATTGACTACACCGATTACCGGGCTACCTGGAAGGCGAATAGCGGGTTCGCTCCGGACTTT
CTGCCGGCCAGCACCGACGGTTTCAACCAGATTTCTTCGGTGGAGAAAGGCGCCAAGGACACTGATGTCGTCCTGACGTT
TAAGACCACCTACCCCGACTGGACGACTGTCCTCAGCACGGTGCTGCCGAAGGAGGGCGTCAAGGATCCCCACACCTTCA
ACGACGGGTGGAAGACGCTAAACCCCGACTGGCTCGCTGGCCCGTTTATCCCGATGAAAGTCGATGAGGCGTCCAAGACT
CTGACCGTCAAGCGCAATGGCAAGTGGTGGGGCGACAAGTCGAAGCTCGATACCGTCAGTTTCAAGGCGATGGATAGCGC
CACCCAGACCAAGGCGTTCGCTAACAAGGAGATTGACGCCGCCTCAAACATCACCACTAAGGATGGCTATCGGACTGCCC
AGAAGCGTCCAGATGTCGAGATGCGTCAGGCTGGTTCGTTGCAGAGGCGCCATTTCACCTTCAACACCAAGTCGACGATG
TTGTCCGATAAGAAGGTGCGTCAGGCTATCGTCAAGGGCATCAACCGTCCCGCTATCGCCAAGTCGGACCTTGCGGGCCT
GCCGGTTAGTCCTGACACTCTGATGCTGGGCAACCACTTGTTTATGCCGGGTCAGGCCGGCTACCGGGATAACTCTGCGG
GTTACAACTACGATCCTGAAGCTGCTAAGAAGGGTCTGGACGAGGCCGGCTGGAAGAAGCAGGGTGACTACCGCGTCAAG
GATGGCAAGACTCTGACCATTAATTACGCTAAGTTGACTGGCGTCCCCACCTCCGAGAACGAGGGTGCGCTGTTCAAGCA
GGACATGGCGAAGATCGGCGTGAAGGTCAATCTGGTCAACACCCCGAGTGATTCCTTCATCCAGACCTTGAGCAGCCACT
CCTTCGACGTCATCGCCTTCACCTGGGGCACCGCGTATCCGATGGCTAACATCCGCCAGATCTATGGCGCTGCTGCGGAG
GGGTCCAAGCAGCCGTCGCAGTCCAACTTCTCTCAGCTCCTCGATCCAGAAGTTGAGAAACTCATTTCGAAGATCGACAC
CGAGTCTGATGTCTCAAAGCGCCGCGAGTTGGCTAACCAGGCTGACAAGACCATTTGGGATGACGTCATGACTTTGCCAC
TGTACCGACGTATGTCCCCTACTGCTGTTCCGAAGAACCTGGCGAACTTCGGTGCCTCCACCTTCCAGACCGTGCGCGCA
GAGGACATCGGCTTCCAGAAGTGA

Upstream 100 bases:

>100_bases
TGATCTTCACGGTGGTTTCGTAGGGTGTGATGTCCGGTTTTCGACGCAGAGGCCGGATGATGGCAGGAGCGTCCTGCCAT
TCACTTCACTGGAGGACATC

Downstream 100 bases:

>100_bases
CGATGACATATCTGTGTTGAGAACCGTGCTGGGAATGTTTTCCCGGCATGGTGTTTCGCAAGGTTCTGCTACAGTTGTGC
CGCCGACGTTGGCACAACTG

Product: extracellular solute-binding transport protein, putative oligopeptide-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MRKAIVTPVAVLAVLVMALTGCGQKNQSGGSKQSGVTSESAPMAQLNVVDRGQLKDGGTLRLAIEQLPTEWNPLNVNGDT
ADLGTTIWRFVGATNFDITEDGTPKPNPNYIFSTDVETKGGKQVVTLHLNPKAKWNSGRTIDYTDYRATWKANSGFAPDF
LPASTDGFNQISSVEKGAKDTDVVLTFKTTYPDWTTVLSTVLPKEGVKDPHTFNDGWKTLNPDWLAGPFIPMKVDEASKT
LTVKRNGKWWGDKSKLDTVSFKAMDSATQTKAFANKEIDAASNITTKDGYRTAQKRPDVEMRQAGSLQRRHFTFNTKSTM
LSDKKVRQAIVKGINRPAIAKSDLAGLPVSPDTLMLGNHLFMPGQAGYRDNSAGYNYDPEAAKKGLDEAGWKKQGDYRVK
DGKTLTINYAKLTGVPTSENEGALFKQDMAKIGVKVNLVNTPSDSFIQTLSSHSFDVIAFTWGTAYPMANIRQIYGAAAE
GSKQPSQSNFSQLLDPEVEKLISKIDTESDVSKRRELANQADKTIWDDVMTLPLYRRMSPTAVPKNLANFGASTFQTVRA
EDIGFQK

Sequences:

>Translated_567_residues
MRKAIVTPVAVLAVLVMALTGCGQKNQSGGSKQSGVTSESAPMAQLNVVDRGQLKDGGTLRLAIEQLPTEWNPLNVNGDT
ADLGTTIWRFVGATNFDITEDGTPKPNPNYIFSTDVETKGGKQVVTLHLNPKAKWNSGRTIDYTDYRATWKANSGFAPDF
LPASTDGFNQISSVEKGAKDTDVVLTFKTTYPDWTTVLSTVLPKEGVKDPHTFNDGWKTLNPDWLAGPFIPMKVDEASKT
LTVKRNGKWWGDKSKLDTVSFKAMDSATQTKAFANKEIDAASNITTKDGYRTAQKRPDVEMRQAGSLQRRHFTFNTKSTM
LSDKKVRQAIVKGINRPAIAKSDLAGLPVSPDTLMLGNHLFMPGQAGYRDNSAGYNYDPEAAKKGLDEAGWKKQGDYRVK
DGKTLTINYAKLTGVPTSENEGALFKQDMAKIGVKVNLVNTPSDSFIQTLSSHSFDVIAFTWGTAYPMANIRQIYGAAAE
GSKQPSQSNFSQLLDPEVEKLISKIDTESDVSKRRELANQADKTIWDDVMTLPLYRRMSPTAVPKNLANFGASTFQTVRA
EDIGFQK
>Mature_567_residues
MRKAIVTPVAVLAVLVMALTGCGQKNQSGGSKQSGVTSESAPMAQLNVVDRGQLKDGGTLRLAIEQLPTEWNPLNVNGDT
ADLGTTIWRFVGATNFDITEDGTPKPNPNYIFSTDVETKGGKQVVTLHLNPKAKWNSGRTIDYTDYRATWKANSGFAPDF
LPASTDGFNQISSVEKGAKDTDVVLTFKTTYPDWTTVLSTVLPKEGVKDPHTFNDGWKTLNPDWLAGPFIPMKVDEASKT
LTVKRNGKWWGDKSKLDTVSFKAMDSATQTKAFANKEIDAASNITTKDGYRTAQKRPDVEMRQAGSLQRRHFTFNTKSTM
LSDKKVRQAIVKGINRPAIAKSDLAGLPVSPDTLMLGNHLFMPGQAGYRDNSAGYNYDPEAAKKGLDEAGWKKQGDYRVK
DGKTLTINYAKLTGVPTSENEGALFKQDMAKIGVKVNLVNTPSDSFIQTLSSHSFDVIAFTWGTAYPMANIRQIYGAAAE
GSKQPSQSNFSQLLDPEVEKLISKIDTESDVSKRRELANQADKTIWDDVMTLPLYRRMSPTAVPKNLANFGASTFQTVRA
EDIGFQK

Specific function: Involved In A Nickel Transport System, Probably Represents The Nickel Binder. [C]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=381, Percent_Identity=23.3595800524934, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789966, Length=346, Percent_Identity=25.1445086705202, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62166; Mature: 62166

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAIVTPVAVLAVLVMALTGCGQKNQSGGSKQSGVTSESAPMAQLNVVDRGQLKDGGTL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEE
RLAIEQLPTEWNPLNVNGDTADLGTTIWRFVGATNFDITEDGTPKPNPNYIFSTDVETKG
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCC
GKQVVTLHLNPKAKWNSGRTIDYTDYRATWKANSGFAPDFLPASTDGFNQISSVEKGAKD
CCEEEEEEECCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC
TDVVLTFKTTYPDWTTVLSTVLPKEGVKDPHTFNDGWKTLNPDWLAGPFIPMKVDEASKT
CEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCE
LTVKRNGKWWGDKSKLDTVSFKAMDSATQTKAFANKEIDAASNITTKDGYRTAQKRPDVE
EEEEECCCCCCCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHH
MRQAGSLQRRHFTFNTKSTMLSDKKVRQAIVKGINRPAIAKSDLAGLPVSPDTLMLGNHL
HHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCEE
FMPGQAGYRDNSAGYNYDPEAAKKGLDEAGWKKQGDYRVKDGKTLTINYAKLTGVPTSEN
ECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCC
EGALFKQDMAKIGVKVNLVNTPSDSFIQTLSSHSFDVIAFTWGTAYPMANIRQIYGAAAE
CCCHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GSKQPSQSNFSQLLDPEVEKLISKIDTESDVSKRRELANQADKTIWDDVMTLPLYRRMSP
CCCCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAVPKNLANFGASTFQTVRAEDIGFQK
CCCHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKAIVTPVAVLAVLVMALTGCGQKNQSGGSKQSGVTSESAPMAQLNVVDRGQLKDGGTL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEE
RLAIEQLPTEWNPLNVNGDTADLGTTIWRFVGATNFDITEDGTPKPNPNYIFSTDVETKG
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCC
GKQVVTLHLNPKAKWNSGRTIDYTDYRATWKANSGFAPDFLPASTDGFNQISSVEKGAKD
CCEEEEEEECCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC
TDVVLTFKTTYPDWTTVLSTVLPKEGVKDPHTFNDGWKTLNPDWLAGPFIPMKVDEASKT
CEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCE
LTVKRNGKWWGDKSKLDTVSFKAMDSATQTKAFANKEIDAASNITTKDGYRTAQKRPDVE
EEEEECCCCCCCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHH
MRQAGSLQRRHFTFNTKSTMLSDKKVRQAIVKGINRPAIAKSDLAGLPVSPDTLMLGNHL
HHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCEE
FMPGQAGYRDNSAGYNYDPEAAKKGLDEAGWKKQGDYRVKDGKTLTINYAKLTGVPTSEN
ECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCC
EGALFKQDMAKIGVKVNLVNTPSDSFIQTLSSHSFDVIAFTWGTAYPMANIRQIYGAAAE
CCCHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GSKQPSQSNFSQLLDPEVEKLISKIDTESDVSKRRELANQADKTIWDDVMTLPLYRRMSP
CCCCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAVPKNLANFGASTFQTVRAEDIGFQK
CCCHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]