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The map label for this gene is cls [H]

Identifier: 50843221

GI number: 50843221

Start: 1923523

End: 1924791

Strand: Direct

Name: cls [H]

Synonym: PPA1761

Alternate gene names: 50843221

Gene position: 1923523-1924791 (Clockwise)

Preceding gene: 50843220

Following gene: 50843222

Centisome position: 75.13

GC content: 58.79

Gene sequence:

>1269_bases
GTGCACATGACCCGGGCCCCTCGCCAAAGATTCACTGTTCGCCGCGCACTGCACTGGGTGGCAGGCGGGATGGTTGCCGC
CCAGATGGCTACGATGGCCGGACTGGTCACCTACAACGGCATTAAGCGACGAGGCCGTCGCCCATACCGTTTCCCCACTG
CGCCGGCAGAGCCCCTCAAGGTGTCTGGTCATCAGGTTGAGATCTTCACTTTCGGACGCGATCTTTACGCGGCGATGATC
TTCGACATTAAATCAGCTCAACACACCGTTTACCTTGAGACCTTCATCTGGAAAAACGACGAAGTCGGGCGTCATTTTCG
GCAGGCACTCGTAGAAGCCGCCGCACGCGGGGTCAAGGTCTATGCGATGTGGGACACCTTCGCCAACCTCGTTGTGGACC
CACGATTCTTTCGGCAGCTCGAAGGGGTGCAGGTGCGAGCCCAACCTCTCGTAACCCCCTCATGGATACCGACGCTACGC
AATCTGGGGCGAGACCACCGCAAGCTCCTCATCGTCGATTCCAAGGTCGCCTACATCGGTGGGTACAACATCGGCTCCCT
CTACGCCGACCGCTGGCGTGACACCCACGCTCGTATTACCGGGCCTGCCGTCGGGGAGCTCGAAAACGTCTTTGTTGACA
TGTGGAATCAACGCCCCAAGGGGGCCCTCATCTCCCGGCGCAACCAGCCCGTACTACCTTCTCCCGGCGTCCGCTATTGG
GATACGCCTTTCGCTGTACACCGCAACTCCCCCCGGATGGCGGTCTATCCCATCCGGAACATGTACCTGGAGGCCATCGA
CCGCGCGAGTGAGCGAATCTGGATGACCCAGGGCTACCTCATCCCCGATGACGACGTTGTCGCAGCCCTCCACCAAGCCG
CATCTCGCGGGGTTGACGTTAGGATCGTCATTCCGGCTGAATCGAACCATGTCATCGCCGACTGGCTGAGCCGGGGCTAC
TACGACCGCCTCCTCCACCAGGGTGTGCGACTGTTCCTTTACCAGGGAGCTATGGTCCACGCGAAGACCTGCACTATTGA
CGGCATTTGGTCCACGATTGGTACAGCGAACCTCGACCGGTTATCCCTCCAAGGTAACTATGAGGTAAACGTCGCTATCA
CTGACTCCACCGTCGCCCAACGAATGGCTGAGATTTTCGAGATCGACATGGCCAACTGTGTGGAACTGACCTTAGACGAA
TGGCAGTCGCGCTCTCACATAGCGAAACTCACTGAAGCTCTACTGTCACCTTGGCGACCATTTTTCTAG

Upstream 100 bases:

>100_bases
ACTGTGGCGACAAGCGCCCAGACCATCCATAACACCACATACCACTTCCGGACCCCACCGCCGGCTGCCTCGCCGCCAGT
GCACGAAGGCCCGGTACTCT

Downstream 100 bases:

>100_bases
AATTCCGTCTGCAGCGTAATAAAAATACGTAACCCCTTGTCAGGGCATAGTTGACTATAGTAACATATGACTATATTTAC
AGTATTTTTCATCCATCTTC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MHMTRAPRQRFTVRRALHWVAGGMVAAQMATMAGLVTYNGIKRRGRRPYRFPTAPAEPLKVSGHQVEIFTFGRDLYAAMI
FDIKSAQHTVYLETFIWKNDEVGRHFRQALVEAAARGVKVYAMWDTFANLVVDPRFFRQLEGVQVRAQPLVTPSWIPTLR
NLGRDHRKLLIVDSKVAYIGGYNIGSLYADRWRDTHARITGPAVGELENVFVDMWNQRPKGALISRRNQPVLPSPGVRYW
DTPFAVHRNSPRMAVYPIRNMYLEAIDRASERIWMTQGYLIPDDDVVAALHQAASRGVDVRIVIPAESNHVIADWLSRGY
YDRLLHQGVRLFLYQGAMVHAKTCTIDGIWSTIGTANLDRLSLQGNYEVNVAITDSTVAQRMAEIFEIDMANCVELTLDE
WQSRSHIAKLTEALLSPWRPFF

Sequences:

>Translated_422_residues
MHMTRAPRQRFTVRRALHWVAGGMVAAQMATMAGLVTYNGIKRRGRRPYRFPTAPAEPLKVSGHQVEIFTFGRDLYAAMI
FDIKSAQHTVYLETFIWKNDEVGRHFRQALVEAAARGVKVYAMWDTFANLVVDPRFFRQLEGVQVRAQPLVTPSWIPTLR
NLGRDHRKLLIVDSKVAYIGGYNIGSLYADRWRDTHARITGPAVGELENVFVDMWNQRPKGALISRRNQPVLPSPGVRYW
DTPFAVHRNSPRMAVYPIRNMYLEAIDRASERIWMTQGYLIPDDDVVAALHQAASRGVDVRIVIPAESNHVIADWLSRGY
YDRLLHQGVRLFLYQGAMVHAKTCTIDGIWSTIGTANLDRLSLQGNYEVNVAITDSTVAQRMAEIFEIDMANCVELTLDE
WQSRSHIAKLTEALLSPWRPFF
>Mature_422_residues
MHMTRAPRQRFTVRRALHWVAGGMVAAQMATMAGLVTYNGIKRRGRRPYRFPTAPAEPLKVSGHQVEIFTFGRDLYAAMI
FDIKSAQHTVYLETFIWKNDEVGRHFRQALVEAAARGVKVYAMWDTFANLVVDPRFFRQLEGVQVRAQPLVTPSWIPTLR
NLGRDHRKLLIVDSKVAYIGGYNIGSLYADRWRDTHARITGPAVGELENVFVDMWNQRPKGALISRRNQPVLPSPGVRYW
DTPFAVHRNSPRMAVYPIRNMYLEAIDRASERIWMTQGYLIPDDDVVAALHQAASRGVDVRIVIPAESNHVIADWLSRGY
YDRLLHQGVRLFLYQGAMVHAKTCTIDGIWSTIGTANLDRLSLQGNYEVNVAITDSTVAQRMAEIFEIDMANCVELTLDE
WQSRSHIAKLTEALLSPWRPFF

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=381, Percent_Identity=27.0341207349081, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787007, Length=357, Percent_Identity=29.4117647058824, Blast_Score=127, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 48165; Mature: 48165

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMTRAPRQRFTVRRALHWVAGGMVAAQMATMAGLVTYNGIKRRGRRPYRFPTAPAEPLK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
VSGHQVEIFTFGRDLYAAMIFDIKSAQHTVYLETFIWKNDEVGRHFRQALVEAAARGVKV
ECCCEEEEEEECHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCEEE
YAMWDTFANLVVDPRFFRQLEGVQVRAQPLVTPSWIPTLRNLGRDHRKLLIVDSKVAYIG
EEEHHHHHHHHCCHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCEEEEC
GYNIGSLYADRWRDTHARITGPAVGELENVFVDMWNQRPKGALISRRNQPVLPSPGVRYW
CCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCC
DTPFAVHRNSPRMAVYPIRNMYLEAIDRASERIWMTQGYLIPDDDVVAALHQAASRGVDV
CCCEEEECCCCCEEEEHHHHHHHHHHHHHHHEEEEECCEECCCHHHHHHHHHHHHCCCEE
RIVIPAESNHVIADWLSRGYYDRLLHQGVRLFLYQGAMVHAKTCTIDGIWSTIGTANLDR
EEEEECCCCCCHHHHHHCCHHHHHHHHHHHEEEECCCEEEEEEEEECHHHHHHCCCCCCE
LSLQGNYEVNVAITDSTVAQRMAEIFEIDMANCVELTLDEWQSRSHIAKLTEALLSPWRP
EEECCCEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FF
CC
>Mature Secondary Structure
MHMTRAPRQRFTVRRALHWVAGGMVAAQMATMAGLVTYNGIKRRGRRPYRFPTAPAEPLK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
VSGHQVEIFTFGRDLYAAMIFDIKSAQHTVYLETFIWKNDEVGRHFRQALVEAAARGVKV
ECCCEEEEEEECHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCEEE
YAMWDTFANLVVDPRFFRQLEGVQVRAQPLVTPSWIPTLRNLGRDHRKLLIVDSKVAYIG
EEEHHHHHHHHCCHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCEEEEC
GYNIGSLYADRWRDTHARITGPAVGELENVFVDMWNQRPKGALISRRNQPVLPSPGVRYW
CCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCC
DTPFAVHRNSPRMAVYPIRNMYLEAIDRASERIWMTQGYLIPDDDVVAALHQAASRGVDV
CCCEEEECCCCCEEEEHHHHHHHHHHHHHHHEEEEECCEECCCHHHHHHHHHHHHCCCEE
RIVIPAESNHVIADWLSRGYYDRLLHQGVRLFLYQGAMVHAKTCTIDGIWSTIGTANLDR
EEEEECCCCCCHHHHHHCCHHHHHHHHHHHEEEECCCEEEEEEEEECHHHHHHCCCCCCE
LSLQGNYEVNVAITDSTVAQRMAEIFEIDMANCVELTLDEWQSRSHIAKLTEALLSPWRP
EEECCCEEEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10585141 [H]