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The map label for this gene is folD

Identifier: 50843203

GI number: 50843203

Start: 1902760

End: 1903623

Strand: Reverse

Name: folD

Synonym: PPA1743

Alternate gene names: 50843203

Gene position: 1903623-1902760 (Counterclockwise)

Preceding gene: 50843204

Following gene: 50843202

Centisome position: 74.35

GC content: 62.96

Gene sequence:

>864_bases
ATGACCGCCCAGAAACTCGACGGCAAGGCCACTGCCGCCGCGATCAAGTCCGAACTGGCCGAACGCGTAGCAGCTCTGCG
CGATCAAGGTGTCGTACCGGGACTGGGCACCGTGCTCGTCGGGGAGGACCCGGCTTCGCACCAGTACGTCGCTGGCAAAC
ATCGCGACTGCGCGCAAGTAGGCATTGAATCGATTCGTGTTGATTTGCCTGCCGACGTCACCGAGGAGGAACTTGCTCAA
AAGATCCGCGGGCTCAATGCCAACCCGCATTGCACCGGTTACATTGTTCAGCTACCGCTGCCACGTCATATCGACACGAA
CTGGGCGCTTAACCTCATCGACCCTAACAAGGACGCCGACGGCTTGACCCCGGCATCACTGGGGCGTCTTGTCCTCAACG
AGCCGGCCCCGCTACCGTGCACCCCGCGCGGCATCGTCGAGTTGCTGACCCGTCATGGCATCGAGCTGCCGGGTGCCAAC
GTCTGCGTGGTCGGACGCGGTACGACGGTGGGACGTCCGCTCGGGCTGCTGCTGACCCGTCGTTCCGAGAACTGCACCGT
CACCCTGTGTCACACCGGGACCCGTAACCTTGCCGAACACACCCGGCAGGCCGACATCATTATCGGCGCTGCTGGGTCGC
CGGGACTCATTAATGCCGACATGATCCGTGAAGGGGCAGTCCTCGTGGATGTCGGTGTGTCACGGACCCCCGAGGGGAAG
ATCCAGGGCGATTTTACTGCCGACGTGTGGGAGAAGGCCGCGTGGGTCTCTCCGAACCCCGGTGGCGTTGGACCGATGAC
CCGCGCCATGTTGCTATCTAACGTGGTAGACCGGGCTGAACGGCTCACCGCGGATCCTTCATGA

Upstream 100 bases:

>100_bases
CGATTGATCGGGTTATTGACGTGGCCACGATGGTGGTCGAGGGAGCGCGGTTCTCGTGGAAAGATAGGGACCTGTTGAGG
CCGTGACACAAGGAGGATCC

Downstream 100 bases:

>100_bases
GGCAGCCGCGCCACGCTGCTGAGGCGCCCGTCAGTACCGCGACTCGACATGCTCGACGGAGCGAGCGTTGCGCTGAATCT
AAGGAGCATCTTCAATCTTG

Product: methylenetetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTAQKLDGKATAAAIKSELAERVAALRDQGVVPGLGTVLVGEDPASHQYVAGKHRDCAQVGIESIRVDLPADVTEEELAQ
KIRGLNANPHCTGYIVQLPLPRHIDTNWALNLIDPNKDADGLTPASLGRLVLNEPAPLPCTPRGIVELLTRHGIELPGAN
VCVVGRGTTVGRPLGLLLTRRSENCTVTLCHTGTRNLAEHTRQADIIIGAAGSPGLINADMIREGAVLVDVGVSRTPEGK
IQGDFTADVWEKAAWVSPNPGGVGPMTRAMLLSNVVDRAERLTADPS

Sequences:

>Translated_287_residues
MTAQKLDGKATAAAIKSELAERVAALRDQGVVPGLGTVLVGEDPASHQYVAGKHRDCAQVGIESIRVDLPADVTEEELAQ
KIRGLNANPHCTGYIVQLPLPRHIDTNWALNLIDPNKDADGLTPASLGRLVLNEPAPLPCTPRGIVELLTRHGIELPGAN
VCVVGRGTTVGRPLGLLLTRRSENCTVTLCHTGTRNLAEHTRQADIIIGAAGSPGLINADMIREGAVLVDVGVSRTPEGK
IQGDFTADVWEKAAWVSPNPGGVGPMTRAMLLSNVVDRAERLTADPS
>Mature_286_residues
TAQKLDGKATAAAIKSELAERVAALRDQGVVPGLGTVLVGEDPASHQYVAGKHRDCAQVGIESIRVDLPADVTEEELAQK
IRGLNANPHCTGYIVQLPLPRHIDTNWALNLIDPNKDADGLTPASLGRLVLNEPAPLPCTPRGIVELLTRHGIELPGANV
CVVGRGTTVGRPLGLLLTRRSENCTVTLCHTGTRNLAEHTRQADIIIGAAGSPGLINADMIREGAVLVDVGVSRTPEGKI
QGDFTADVWEKAAWVSPNPGGVGPMTRAMLLSNVVDRAERLTADPS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=42.8082191780822, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=40.7534246575342, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=36.734693877551, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI36796743, Length=174, Percent_Identity=27.0114942528736, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=45.2898550724638, Blast_Score=229, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=34.6938775510204, Blast_Score=150, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=40.0696864111498, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=306, Percent_Identity=34.640522875817, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=42.9530201342282, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=42.9530201342282, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=40.0684931506849, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=40.0684931506849, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=40.0684931506849, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=40.0684931506849, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_PROAC (Q6A6Z2)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056430.1
- HSSP:   O50385
- ProteinModelPortal:   Q6A6Z2
- SMR:   Q6A6Z2
- GeneID:   2932227
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1743
- NMPDR:   fig|267747.1.peg.1707
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   CLSK2764148
- BioCyc:   PACN267747:PPA1743-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30297; Mature: 30166

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQKLDGKATAAAIKSELAERVAALRDQGVVPGLGTVLVGEDPASHQYVAGKHRDCAQV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEECCCCCCHHHH
GIESIRVDLPADVTEEELAQKIRGLNANPHCTGYIVQLPLPRHIDTNWALNLIDPNKDAD
CHHHEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCC
GLTPASLGRLVLNEPAPLPCTPRGIVELLTRHGIELPGANVCVVGRGTTVGRPLGLLLTR
CCCHHHHCHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEE
RSENCTVTLCHTGTRNLAEHTRQADIIIGAAGSPGLINADMIREGAVLVDVGVSRTPEGK
CCCCCEEEEEECCCHHHHHHCCCCCEEEECCCCCCCEEHHHHHCCCEEEEECCCCCCCCC
IQGDFTADVWEKAAWVSPNPGGVGPMTRAMLLSNVVDRAERLTADPS
CCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TAQKLDGKATAAAIKSELAERVAALRDQGVVPGLGTVLVGEDPASHQYVAGKHRDCAQV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEECCCCCCHHHH
GIESIRVDLPADVTEEELAQKIRGLNANPHCTGYIVQLPLPRHIDTNWALNLIDPNKDAD
CHHHEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCC
GLTPASLGRLVLNEPAPLPCTPRGIVELLTRHGIELPGANVCVVGRGTTVGRPLGLLLTR
CCCHHHHCHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEE
RSENCTVTLCHTGTRNLAEHTRQADIIIGAAGSPGLINADMIREGAVLVDVGVSRTPEGK
CCCCCEEEEEECCCHHHHHHCCCCCEEEECCCCCCCEEHHHHHCCCEEEEECCCCCCCCC
IQGDFTADVWEKAAWVSPNPGGVGPMTRAMLLSNVVDRAERLTADPS
CCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA