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The map label for this gene is mdh

Identifier: 50843200

GI number: 50843200

Start: 1899521

End: 1900504

Strand: Direct

Name: mdh

Synonym: PPA1740

Alternate gene names: 50843200

Gene position: 1899521-1900504 (Clockwise)

Preceding gene: 50843199

Following gene: 50843201

Centisome position: 74.19

GC content: 61.48

Gene sequence:

>984_bases
ATGACCCAGACCCCAGTCAAGATTGCCGTCACCGGAGCTGCTGGCCAGATTTGCTACAGCTTGTTGTTCCGCATTGCTTC
CGGTTCGCTGCTCGGAGACACTCCTATCGAGCTTCGCCTTCTCGAGATCACCCCGGCCCTCAAAGCCCTTGAGGGCGTCG
TCATGGAGCTTGACGACTGCGCCTTCGGCAATCTCGTGAATATCGAGATCGGTGACGATCCGAAGAAGGTCTTTGACGGA
GTTAACGCCGCCTTCCTCGTCGGCGCCATGCCCCGCAAGGCGGGTATGGAGCGTTCCGATCTGCTCACCAAGAACGGGGC
TATCTTCACCGCCCAAGGCAAGGCCCTCAACGACGTCGCCGCCGACGACGTCCGCGTTCTCGTCACCGGTAACCCCGCTA
ACACCAACGCCCTCATCGCTGCTACCAACGCGGTCGACATCCCGAACAACCACTTCGCCGCCTTGACTCGTCTAGATCAC
AACCGCGCCAAGACGCAGCTTGCCCGCAAGACCGGAAAGACTGTCAATGACGTCCGTCACATGACCATCTGGGGCAACCA
CTCCTCCACCCAGTACCCCGACGTCTTCCACGCCGAGGTCGCCGGCCAGAAGGCAACCAACCTGGTGAACGAAGCTTGGA
TCGAGAACGAGTTCATCCCAACCGTCGCCAAACGTGGCGCTGCCATCATCGACGCGCGCGGCGCCTCCTCGGCTGCCTCG
GCCGCTAACGCCACCGTCGAGTGCATGCGCGACTGGATGGGCTCCACTCCTGAGGGAGACTGGGTGTCCATGGCGATTCC
GTCTGACGGCTCTTACGGCGTGCCCGAGGGCCTCATCTCTTCCTTCCCGGTGACCATCACCAACGGCAAGGTCGAGATTG
TTCAGGGTCTTGACATCGACGACTTCTCGCGCGCCAAGATCGACGCCTCTGCCAAGGAGCTGGCCGACGAACGTGACGCC
GTCAAGGAACTCGGCCTCATCTGA

Upstream 100 bases:

>100_bases
CATTCTTGATACGAATAAGTCCCCCGGCCAACTTCGCGTCGTCACCGCGAGTAGGCTTCAAAATAGCCAAGTGTCATCGA
GGACTTTCAAGGAGAGATCC

Downstream 100 bases:

>100_bases
TCGACCCGACGAACGGGCCCGGAAAACGTCAAGTTTCCGGGCCCGTTCTGCGCGTCACGGCGTGAAACTTCGATGGTTTC
GGCCGGTAACCGAGCGTCGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTQTPVKIAVTGAAGQICYSLLFRIASGSLLGDTPIELRLLEITPALKALEGVVMELDDCAFGNLVNIEIGDDPKKVFDG
VNAAFLVGAMPRKAGMERSDLLTKNGAIFTAQGKALNDVAADDVRVLVTGNPANTNALIAATNAVDIPNNHFAALTRLDH
NRAKTQLARKTGKTVNDVRHMTIWGNHSSTQYPDVFHAEVAGQKATNLVNEAWIENEFIPTVAKRGAAIIDARGASSAAS
AANATVECMRDWMGSTPEGDWVSMAIPSDGSYGVPEGLISSFPVTITNGKVEIVQGLDIDDFSRAKIDASAKELADERDA
VKELGLI

Sequences:

>Translated_327_residues
MTQTPVKIAVTGAAGQICYSLLFRIASGSLLGDTPIELRLLEITPALKALEGVVMELDDCAFGNLVNIEIGDDPKKVFDG
VNAAFLVGAMPRKAGMERSDLLTKNGAIFTAQGKALNDVAADDVRVLVTGNPANTNALIAATNAVDIPNNHFAALTRLDH
NRAKTQLARKTGKTVNDVRHMTIWGNHSSTQYPDVFHAEVAGQKATNLVNEAWIENEFIPTVAKRGAAIIDARGASSAAS
AANATVECMRDWMGSTPEGDWVSMAIPSDGSYGVPEGLISSFPVTITNGKVEIVQGLDIDDFSRAKIDASAKELADERDA
VKELGLI
>Mature_326_residues
TQTPVKIAVTGAAGQICYSLLFRIASGSLLGDTPIELRLLEITPALKALEGVVMELDDCAFGNLVNIEIGDDPKKVFDGV
NAAFLVGAMPRKAGMERSDLLTKNGAIFTAQGKALNDVAADDVRVLVTGNPANTNALIAATNAVDIPNNHFAALTRLDHN
RAKTQLARKTGKTVNDVRHMTIWGNHSSTQYPDVFHAEVAGQKATNLVNEAWIENEFIPTVAKRGAAIIDARGASSAASA
ANATVECMRDWMGSTPEGDWVSMAIPSDGSYGVPEGLISSFPVTITNGKVEIVQGLDIDDFSRAKIDASAKELADERDAV
KELGLI

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=328, Percent_Identity=53.3536585365854, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI89886456, Length=329, Percent_Identity=24.6200607902736, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17561064, Length=322, Percent_Identity=52.7950310559006, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI32566798, Length=259, Percent_Identity=50.1930501930502, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=53.5294117647059, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24583394, Length=321, Percent_Identity=51.7133956386293, Blast_Score=308, Evalue=3e-84,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_PROAC (Q6A6Z5)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056427.1
- ProteinModelPortal:   Q6A6Z5
- SMR:   Q6A6Z5
- GeneID:   2933187
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1740
- NMPDR:   fig|267747.1.peg.1704
- HOGENOM:   HBG289884
- OMA:   PQGRAIN
- ProtClustDB:   PRK05442
- BioCyc:   PACN267747:PPA1740-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34687; Mature: 34555

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTPVKIAVTGAAGQICYSLLFRIASGSLLGDTPIELRLLEITPALKALEGVVMELDDC
CCCCCEEEEEECCHHHHHHHHHHHHHCCCEECCCCCEEEEEEECHHHHHHHHHHHHHCCC
AFGNLVNIEIGDDPKKVFDGVNAAFLVGAMPRKAGMERSDLLTKNGAIFTAQGKALNDVA
CCCCEEEEEECCCHHHHHCCCCCEEEEECCCHHHCCCHHHHHCCCCEEEEECCCCCCCCC
ADDVRVLVTGNPANTNALIAATNAVDIPNNHFAALTRLDHNRAKTQLARKTGKTVNDVRH
CCCEEEEEECCCCCCCEEEEEECEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHEEE
MTIWGNHSSTQYPDVFHAEVAGQKATNLVNEAWIENEFIPTVAKRGAAIIDARGASSAAS
EEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCCCHHH
AANATVECMRDWMGSTPEGDWVSMAIPSDGSYGVPEGLISSFPVTITNGKVEIVQGLDID
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCEEEEEECCCCC
DFSRAKIDASAKELADERDAVKELGLI
CCCHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQTPVKIAVTGAAGQICYSLLFRIASGSLLGDTPIELRLLEITPALKALEGVVMELDDC
CCCCEEEEEECCHHHHHHHHHHHHHCCCEECCCCCEEEEEEECHHHHHHHHHHHHHCCC
AFGNLVNIEIGDDPKKVFDGVNAAFLVGAMPRKAGMERSDLLTKNGAIFTAQGKALNDVA
CCCCEEEEEECCCHHHHHCCCCCEEEEECCCHHHCCCHHHHHCCCCEEEEECCCCCCCCC
ADDVRVLVTGNPANTNALIAATNAVDIPNNHFAALTRLDHNRAKTQLARKTGKTVNDVRH
CCCEEEEEECCCCCCCEEEEEECEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHEEE
MTIWGNHSSTQYPDVFHAEVAGQKATNLVNEAWIENEFIPTVAKRGAAIIDARGASSAAS
EEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCCCHHH
AANATVECMRDWMGSTPEGDWVSMAIPSDGSYGVPEGLISSFPVTITNGKVEIVQGLDID
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCEEEEEECCCCC
DFSRAKIDASAKELADERDAVKELGLI
CCCHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA