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The map label for this gene is purU [H]

Identifier: 50843199

GI number: 50843199

Start: 1898491

End: 1899342

Strand: Direct

Name: purU [H]

Synonym: PPA1739

Alternate gene names: 50843199

Gene position: 1898491-1899342 (Clockwise)

Preceding gene: 50843187

Following gene: 50843200

Centisome position: 74.15

GC content: 59.27

Gene sequence:

>852_bases
ATGTCGGAATCCCATGAGCTCGTTGTCACCTGGACTAGCCCGGATCGCCCCGGACTTGTCCATGCCGTTACCGGGGCGTG
CGCCCAGGTGGGCGGAAACCTCACAGAGTGTCAGCAATTCACTAGCACCGACACCGGAAACTTCTTCATCCGGTTGCAGG
TCGAGTCAGCGAGCAGCCGCGCCGACCTCGAGTCGGCCGTCAGCGAGCTCGCCGGAAAGTGCAACGCTACTGTCCACGTC
GACGAGTTGGGGCGTCCCGTCCGTACCCTCATCTTGGCCTCCAAAGCGTCCCATTGTCTGTCTCACTTGCTGTTTAACCG
GGACGCTGGTCGGCTGCCAATCGACGTTGTCCAGGTCATGGCGAATCATCCTGACCTCGCCGACTTGACTGCTTTCCATA
AGGTTCCGTTCCGGTGGCAAAAAGTCGACCGCGAGTCCAAGACGTCTTTCGAGCAGGAGGTATTGCGCACCGTCGGGGAC
CTTGACGTCGAGCTCGTCGTATTGGCTCGCTATATGCAGATCCTTTCTCCTGAACTGTGTGAGCAACTATCAGGACGGTG
CATCAACATTCATCATTCTTTCCTGCCCGGTTTCAAAGGTGCCAACCCGTACCGCCAAGCCCATTCCCGCGGGGTCAAGC
TCATTGGCGCGACGGCCCACTTCGTCACTGTCGACCTCGACGAGGGCCCCATCATCGAACAGCGAGTGCAACGGGTCAAC
CATTCTCAGACGGTGGCTCAACTCACCGCCGTCGGCCAGGACACCGAATCTGCCACCCTTAACGAGGCCGTCCGTCTGTT
CGCTGAGCACCGCACTTTCCTAGACGGAATGCGCACCGTCATCTTGCAGTAG

Upstream 100 bases:

>100_bases
TCTGACACACCGAAAAGGAACACCGTAGAACACCGAATTGCCGTCATCCAGTTCGCGAGATATCGCATCCGGTGTGACGA
GGCCGACTACTGTACCGGTC

Downstream 100 bases:

>100_bases
CGGTACCTCCTGACGCCTCCACCAGGGCCGTCACCCGATCCCACATGGGGGCCACCACCTGGCCCCCATGGGCGAGGTCA
TTCTTGATACGAATAAGTCC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSESHELVVTWTSPDRPGLVHAVTGACAQVGGNLTECQQFTSTDTGNFFIRLQVESASSRADLESAVSELAGKCNATVHV
DELGRPVRTLILASKASHCLSHLLFNRDAGRLPIDVVQVMANHPDLADLTAFHKVPFRWQKVDRESKTSFEQEVLRTVGD
LDVELVVLARYMQILSPELCEQLSGRCINIHHSFLPGFKGANPYRQAHSRGVKLIGATAHFVTVDLDEGPIIEQRVQRVN
HSQTVAQLTAVGQDTESATLNEAVRLFAEHRTFLDGMRTVILQ

Sequences:

>Translated_283_residues
MSESHELVVTWTSPDRPGLVHAVTGACAQVGGNLTECQQFTSTDTGNFFIRLQVESASSRADLESAVSELAGKCNATVHV
DELGRPVRTLILASKASHCLSHLLFNRDAGRLPIDVVQVMANHPDLADLTAFHKVPFRWQKVDRESKTSFEQEVLRTVGD
LDVELVVLARYMQILSPELCEQLSGRCINIHHSFLPGFKGANPYRQAHSRGVKLIGATAHFVTVDLDEGPIIEQRVQRVN
HSQTVAQLTAVGQDTESATLNEAVRLFAEHRTFLDGMRTVILQ
>Mature_282_residues
SESHELVVTWTSPDRPGLVHAVTGACAQVGGNLTECQQFTSTDTGNFFIRLQVESASSRADLESAVSELAGKCNATVHVD
ELGRPVRTLILASKASHCLSHLLFNRDAGRLPIDVVQVMANHPDLADLTAFHKVPFRWQKVDRESKTSFEQEVLRTVGDL
DVELVVLARYMQILSPELCEQLSGRCINIHHSFLPGFKGANPYRQAHSRGVKLIGATAHFVTVDLDEGPIIEQRVQRVNH
SQTVAQLTAVGQDTESATLNEAVRLFAEHRTFLDGMRTVILQ

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=202, Percent_Identity=30.1980198019802, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI209869995, Length=202, Percent_Identity=30.1980198019802, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI209869993, Length=202, Percent_Identity=30.1980198019802, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=36.5591397849462, Blast_Score=187, Evalue=4e-49,
Organism=Escherichia coli, GI1788846, Length=141, Percent_Identity=34.0425531914894, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=198, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31214; Mature: 31083

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESHELVVTWTSPDRPGLVHAVTGACAQVGGNLTECQQFTSTDTGNFFIRLQVESASSR
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCH
ADLESAVSELAGKCNATVHVDELGRPVRTLILASKASHCLSHLLFNRDAGRLPIDVVQVM
HHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ANHPDLADLTAFHKVPFRWQKVDRESKTSFEQEVLRTVGDLDVELVVLARYMQILSPELC
HCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHH
EQLSGRCINIHHSFLPGFKGANPYRQAHSRGVKLIGATAHFVTVDLDEGPIIEQRVQRVN
HHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHHC
HSQTVAQLTAVGQDTESATLNEAVRLFAEHRTFLDGMRTVILQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SESHELVVTWTSPDRPGLVHAVTGACAQVGGNLTECQQFTSTDTGNFFIRLQVESASSR
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCH
ADLESAVSELAGKCNATVHVDELGRPVRTLILASKASHCLSHLLFNRDAGRLPIDVVQVM
HHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ANHPDLADLTAFHKVPFRWQKVDRESKTSFEQEVLRTVGDLDVELVVLARYMQILSPELC
HCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHH
EQLSGRCINIHHSFLPGFKGANPYRQAHSRGVKLIGATAHFVTVDLDEGPIIEQRVQRVN
HHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHHC
HSQTVAQLTAVGQDTESATLNEAVRLFAEHRTFLDGMRTVILQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]